First-Void Urine Microbiome in Women with <i>Chlamydia trachomatis</i> Infection
Background: <i>Chlamydia trachomatis</i> (CT) is the agent of the most common bacterial sexually transmitted infection worldwide. Until now, little information is available about the microbial composition of urine samples during CT urethritis. Therefore, in this study, we characterized t...
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/10/5625 |
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| author | Valeria Gaspari Camilla Ceccarani Marco Severgnini Gionathan Orioni Tania Camboni Luca Laghi Sara Morselli Claudio Foschi Antonella Marangoni Clarissa Consolandi Bianca Maria Piraccini |
| author_facet | Valeria Gaspari Camilla Ceccarani Marco Severgnini Gionathan Orioni Tania Camboni Luca Laghi Sara Morselli Claudio Foschi Antonella Marangoni Clarissa Consolandi Bianca Maria Piraccini |
| author_sort | Valeria Gaspari |
| collection | DOAJ |
| description | Background: <i>Chlamydia trachomatis</i> (CT) is the agent of the most common bacterial sexually transmitted infection worldwide. Until now, little information is available about the microbial composition of urine samples during CT urethritis. Therefore, in this study, we characterized the microbiome and metabolome profiles of first-void urines in a cohort of women with CT urethral infection attending an STI clinic. Methods: Based on CT positivity by nucleic acid amplification techniques on urine samples, the enrolled women were divided into two groups, i.e., “CT-negative” (<i>n</i> = 21) and “CT-positive” (<i>n</i> = 11). Urine samples were employed for (i) the microbiome profile analysis by means of 16s rRNA gene sequencing and (ii) the metabolome analysis by <sup>1</sup>H-NMR. Results: Irrespective of CT infection, the microbiome of first-void urines was mainly dominated by <i>Lactobacillus</i>, <i>L. iners</i> and <i>L. crispatus</i> being the most represented species. CT-positive samples were characterized by reduced microbial biodiversity compared to the controls. Moreover, a significant reduction of the Mycoplasmataceae family—in particular, of the <i>Ureaplasma parvum</i> species—was observed during CT infection. The <i>Chlamydia</i> genus was positively correlated with urine hippurate and lactulose. Conclusions: These data can help elucidate the pathogenesis of chlamydial urogenital infections, as well as to set up innovative diagnostic and therapeutic approaches. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:43:05Z |
| publishDate | 2022-05-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-050f5a9603174c0fae968d464eee8f302023-11-23T11:25:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310562510.3390/ijms23105625First-Void Urine Microbiome in Women with <i>Chlamydia trachomatis</i> InfectionValeria Gaspari0Camilla Ceccarani1Marco Severgnini2Gionathan Orioni3Tania Camboni4Luca Laghi5Sara Morselli6Claudio Foschi7Antonella Marangoni8Clarissa Consolandi9Bianca Maria Piraccini10Department of Dermatology, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, ItalyInstitute of Biomedical Technologies, National Research Council, 20054 Segrate, ItalyInstitute of Biomedical Technologies, National Research Council, 20054 Segrate, ItalyDepartment of Dermatology, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, ItalyInstitute of Biomedical Technologies, National Research Council, 20054 Segrate, ItalyDepartment of Agricultural and Food Sciences, University of Bologna, 47521 Cesena, ItalySection of Microbiology, Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, 40138 Bologna, ItalySection of Microbiology, Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, 40138 Bologna, ItalySection of Microbiology, Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, 40138 Bologna, ItalyInstitute of Biomedical Technologies, National Research Council, 20054 Segrate, ItalyDepartment of Dermatology, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, ItalyBackground: <i>Chlamydia trachomatis</i> (CT) is the agent of the most common bacterial sexually transmitted infection worldwide. Until now, little information is available about the microbial composition of urine samples during CT urethritis. Therefore, in this study, we characterized the microbiome and metabolome profiles of first-void urines in a cohort of women with CT urethral infection attending an STI clinic. Methods: Based on CT positivity by nucleic acid amplification techniques on urine samples, the enrolled women were divided into two groups, i.e., “CT-negative” (<i>n</i> = 21) and “CT-positive” (<i>n</i> = 11). Urine samples were employed for (i) the microbiome profile analysis by means of 16s rRNA gene sequencing and (ii) the metabolome analysis by <sup>1</sup>H-NMR. Results: Irrespective of CT infection, the microbiome of first-void urines was mainly dominated by <i>Lactobacillus</i>, <i>L. iners</i> and <i>L. crispatus</i> being the most represented species. CT-positive samples were characterized by reduced microbial biodiversity compared to the controls. Moreover, a significant reduction of the Mycoplasmataceae family—in particular, of the <i>Ureaplasma parvum</i> species—was observed during CT infection. The <i>Chlamydia</i> genus was positively correlated with urine hippurate and lactulose. Conclusions: These data can help elucidate the pathogenesis of chlamydial urogenital infections, as well as to set up innovative diagnostic and therapeutic approaches.https://www.mdpi.com/1422-0067/23/10/5625<i>Chlamydia trachomatis</i>urineurobiomemicrobiomemetabolomeurethritis |
| spellingShingle | Valeria Gaspari Camilla Ceccarani Marco Severgnini Gionathan Orioni Tania Camboni Luca Laghi Sara Morselli Claudio Foschi Antonella Marangoni Clarissa Consolandi Bianca Maria Piraccini First-Void Urine Microbiome in Women with <i>Chlamydia trachomatis</i> Infection International Journal of Molecular Sciences <i>Chlamydia trachomatis</i> urine urobiome microbiome metabolome urethritis |
| title | First-Void Urine Microbiome in Women with <i>Chlamydia trachomatis</i> Infection |
| title_full | First-Void Urine Microbiome in Women with <i>Chlamydia trachomatis</i> Infection |
| title_fullStr | First-Void Urine Microbiome in Women with <i>Chlamydia trachomatis</i> Infection |
| title_full_unstemmed | First-Void Urine Microbiome in Women with <i>Chlamydia trachomatis</i> Infection |
| title_short | First-Void Urine Microbiome in Women with <i>Chlamydia trachomatis</i> Infection |
| title_sort | first void urine microbiome in women with i chlamydia trachomatis i infection |
| topic | <i>Chlamydia trachomatis</i> urine urobiome microbiome metabolome urethritis |
| url | https://www.mdpi.com/1422-0067/23/10/5625 |
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