Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR
A wide range of light-activated molecules (photoswitches and phototriggers) have been used to the study of computational properties of an isolated neuron by acting pre and postsynaptically. However, new tools are being pursued to elicit a presynaptic calcium influx that triggers the release of neur...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-03-01
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| Series: | Frontiers in Molecular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnmol.2013.00003/full |
| _version_ | 1818467038798020608 |
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| author | Mercè eIzquierdo-Serra Dirk eTrauner Artur eLlobet Pau eGorostiza Pau eGorostiza Pau eGorostiza |
| author_facet | Mercè eIzquierdo-Serra Dirk eTrauner Artur eLlobet Pau eGorostiza Pau eGorostiza Pau eGorostiza |
| author_sort | Mercè eIzquierdo-Serra |
| collection | DOAJ |
| description | A wide range of light-activated molecules (photoswitches and phototriggers) have been used to the study of computational properties of an isolated neuron by acting pre and postsynaptically. However, new tools are being pursued to elicit a presynaptic calcium influx that triggers the release of neurotransmitters, most of them based in calcium-permeable Channelrhodopsin-2 mutants. Here we describe a method to control exocytosis of synaptic vesicles through the use of a light-gated glutamate receptor (LiGluR), which has recently been demonstrated that supports secretion by means of calcium influx in chromaffin cells. Expression of LiGluR in hippocampal neurons enables reversible control of neurotransmission with light, and allows modulating the firing rate of the postsynaptic neuron with the wavelength of illumination. This method may be useful for the determination of the complex transfer function of individual synapses. |
| first_indexed | 2024-04-13T21:04:20Z |
| format | Article |
| id | doaj.art-050f98bc117547048619a9386531b8a2 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-04-13T21:04:20Z |
| publishDate | 2013-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-050f98bc117547048619a9386531b8a22022-12-22T02:30:02ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992013-03-01610.3389/fnmol.2013.0000340517Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluRMercè eIzquierdo-Serra0Dirk eTrauner1Artur eLlobet2Pau eGorostiza3Pau eGorostiza4Pau eGorostiza5Institute for Bioengineering of Catalonia (IBEC)Ludwig-Maximilians-UniversitätBellvitge Institute for Biomedical Research (IDIBELL)Institute for Bioengineering of Catalonia (IBEC)Catalan Institution for Research and Advanced Studies (ICREA)Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)A wide range of light-activated molecules (photoswitches and phototriggers) have been used to the study of computational properties of an isolated neuron by acting pre and postsynaptically. However, new tools are being pursued to elicit a presynaptic calcium influx that triggers the release of neurotransmitters, most of them based in calcium-permeable Channelrhodopsin-2 mutants. Here we describe a method to control exocytosis of synaptic vesicles through the use of a light-gated glutamate receptor (LiGluR), which has recently been demonstrated that supports secretion by means of calcium influx in chromaffin cells. Expression of LiGluR in hippocampal neurons enables reversible control of neurotransmission with light, and allows modulating the firing rate of the postsynaptic neuron with the wavelength of illumination. This method may be useful for the determination of the complex transfer function of individual synapses.http://journal.frontiersin.org/Journal/10.3389/fnmol.2013.00003/fullCalciumneurotransmissionoptogeneticsNeural codingfiring rateOptical control |
| spellingShingle | Mercè eIzquierdo-Serra Dirk eTrauner Artur eLlobet Pau eGorostiza Pau eGorostiza Pau eGorostiza Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR Frontiers in Molecular Neuroscience Calcium neurotransmission optogenetics Neural coding firing rate Optical control |
| title | Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR |
| title_full | Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR |
| title_fullStr | Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR |
| title_full_unstemmed | Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR |
| title_short | Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR |
| title_sort | optical modulation of neurotransmission using calcium photocurrents through the ion channel liglur |
| topic | Calcium neurotransmission optogenetics Neural coding firing rate Optical control |
| url | http://journal.frontiersin.org/Journal/10.3389/fnmol.2013.00003/full |
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