SSR Analysis of Genetic Diversity Among 192 Diploid Potato Cultivars
In potato breeding, it is difficult to improve the traits of interest at the tetraploid level due to the tetrasomic inheritance. A promising alternative is diploid breeding. Thus it is necessary to assess the genetic diversity of diploid potato germplasm for efficient exploration and deployment of d...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2016-05-01
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| Series: | Horticultural Plant Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S246801411630067X |
| _version_ | 1797203205778571264 |
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| author | Xiaoyan Song Chunzhi Zhang Ying Li Shuangshuang Feng Qing Yang Sanwen Huang |
| author_facet | Xiaoyan Song Chunzhi Zhang Ying Li Shuangshuang Feng Qing Yang Sanwen Huang |
| author_sort | Xiaoyan Song |
| collection | DOAJ |
| description | In potato breeding, it is difficult to improve the traits of interest at the tetraploid level due to the tetrasomic inheritance. A promising alternative is diploid breeding. Thus it is necessary to assess the genetic diversity of diploid potato germplasm for efficient exploration and deployment of desirable traits. In this study, we used SSR markers to evaluate the genetic diversity of diploid potato cultivars. To screen polymorphic SSR markers, 55 pairs of SSR primers were employed to amplify 39 cultivars with relatively distant genetic relationships. Among them, 12 SSR markers with high polymorphism located at 12 chromosomes were chosen to evaluate the genetic diversity of 192 diploid potato cultivars. The primers produced 6 to 18 bands with an average of 8.2 bands per primer. In total, 98 bands were amplified from 192 cultivars, and 97 of them were polymorphic. Cluster analysis using UPGMA showed the genetic relationships of all accessions tested: 186 of the 192 accessions could be distinguished by only 12 pairs of SSR primers, and the 192 diploid cultivars were divided into 11 groups, and 83.3% constituted the first group. Clustering results showed relatively low genetic diversity among 192 diploid cultivars, with closer relationship at the molecular level. The results can provide molecular basis for diploid potato breeding. |
| first_indexed | 2024-04-24T08:15:38Z |
| format | Article |
| id | doaj.art-0511834d825f491b8b8167e438dcc166 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:15:38Z |
| publishDate | 2016-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-0511834d825f491b8b8167e438dcc1662024-04-17T03:36:09ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412016-05-012316317110.1016/j.hpj.2016.08.006SSR Analysis of Genetic Diversity Among 192 Diploid Potato CultivarsXiaoyan Song0Chunzhi Zhang1Ying Li2Shuangshuang Feng3Qing Yang4Sanwen Huang5College of Life Science, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Life Science, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Life Science, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaIn potato breeding, it is difficult to improve the traits of interest at the tetraploid level due to the tetrasomic inheritance. A promising alternative is diploid breeding. Thus it is necessary to assess the genetic diversity of diploid potato germplasm for efficient exploration and deployment of desirable traits. In this study, we used SSR markers to evaluate the genetic diversity of diploid potato cultivars. To screen polymorphic SSR markers, 55 pairs of SSR primers were employed to amplify 39 cultivars with relatively distant genetic relationships. Among them, 12 SSR markers with high polymorphism located at 12 chromosomes were chosen to evaluate the genetic diversity of 192 diploid potato cultivars. The primers produced 6 to 18 bands with an average of 8.2 bands per primer. In total, 98 bands were amplified from 192 cultivars, and 97 of them were polymorphic. Cluster analysis using UPGMA showed the genetic relationships of all accessions tested: 186 of the 192 accessions could be distinguished by only 12 pairs of SSR primers, and the 192 diploid cultivars were divided into 11 groups, and 83.3% constituted the first group. Clustering results showed relatively low genetic diversity among 192 diploid cultivars, with closer relationship at the molecular level. The results can provide molecular basis for diploid potato breeding.http://www.sciencedirect.com/science/article/pii/S246801411630067XSSR markersgenetic diversitydiploid potato cultivarsDNA fingerprinting |
| spellingShingle | Xiaoyan Song Chunzhi Zhang Ying Li Shuangshuang Feng Qing Yang Sanwen Huang SSR Analysis of Genetic Diversity Among 192 Diploid Potato Cultivars Horticultural Plant Journal SSR markers genetic diversity diploid potato cultivars DNA fingerprinting |
| title | SSR Analysis of Genetic Diversity Among 192 Diploid Potato Cultivars |
| title_full | SSR Analysis of Genetic Diversity Among 192 Diploid Potato Cultivars |
| title_fullStr | SSR Analysis of Genetic Diversity Among 192 Diploid Potato Cultivars |
| title_full_unstemmed | SSR Analysis of Genetic Diversity Among 192 Diploid Potato Cultivars |
| title_short | SSR Analysis of Genetic Diversity Among 192 Diploid Potato Cultivars |
| title_sort | ssr analysis of genetic diversity among 192 diploid potato cultivars |
| topic | SSR markers genetic diversity diploid potato cultivars DNA fingerprinting |
| url | http://www.sciencedirect.com/science/article/pii/S246801411630067X |
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