IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidis
Objective Salmonella is a Gram-negative, anaerobic, flagellated bacterium of Salmonella type in Enterobacteriaceae family, which is known as a zoonosis infectious agent. In recent years, different types of antibiotics have been used to overcome the Salmonella infection; however, the increasing numbe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Shahid Bahonar University of Kerman
2020-10-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_2739_5858828cc41c777fd8228711b73e0447.pdf |
| _version_ | 1818961615977971712 |
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| author | Shima Eshaghi Mohammadreza Nassiri Mojtaba Tahmoorespour Ali Javadmanesh |
| author_facet | Shima Eshaghi Mohammadreza Nassiri Mojtaba Tahmoorespour Ali Javadmanesh |
| author_sort | Shima Eshaghi |
| collection | DOAJ |
| description | Objective
Salmonella is a Gram-negative, anaerobic, flagellated bacterium of Salmonella type in Enterobacteriaceae family, which is known as a zoonosis infectious agent. In recent years, different types of antibiotics have been used to overcome the Salmonella infection; however, the increasing number of antibiotic-resistant bacteria, the provision of alternative methods seems be necessary. Specific immunoglobulin Y (IgY) originated from egg yolk causes passive immunity in the fetus, and extracting these antibodies from egg yolk is cheaper and more feasible than other antibodies. Numerous studies have shown that the egg yolk IgY has immunogenic function against a wide range of bacterial and viral infections in mammals. The purpose of this study was to produce specific immunoglobulin Y against Salmonella typhimurium and Salmonella enteritidis bacteria in laying hens.
Materials and Methods
In the current study, six pullets were inoculated and boosted with one milliliter of killed Salmonella typhimurium and Salmonella enteritidis. Injections were done fortnightly until one week before egg production. After breast muscle injection of chickens, the eggs were collected, and the IgY was extracted from yolk by the Polson method with polyethylene glycol 6000. The produced IgY was approved by SDS-PAGE method. The specific indirect ELISA done by specificity test for purified IgY also approved the specific IgY against both Salmonella typhimurium and Salmonella enteritidis.
Results
The results of SDS - PAGE demonstrated the presence of two protein bands of 27 and 67 kDa representing light and heavy chains of IgY, respectively. The specific indirect ELISA method proved that the specific IgY against Salmonella typhimurium and Salmonella enteritidis was produced in the egg yolk.
Conclusions
It might be possible to administer the egg yolk containing specific IgY against both Salmonella typhimurium and Salmonella enteritidis, to induce passive immunity for preventing some pathogenic diseases in calves such as diarrhea. |
| first_indexed | 2024-12-20T12:16:15Z |
| format | Article |
| id | doaj.art-0513179ee05a460eb3673ad0b8d370a6 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-12-20T12:16:15Z |
| publishDate | 2020-10-01 |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-0513179ee05a460eb3673ad0b8d370a62022-12-21T19:41:06ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002020-10-0112317719010.22103/jab.2020.16014.12442739IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidisShima Eshaghi0Mohammadreza Nassiri1Mojtaba Tahmoorespour2Ali Javadmanesh3Department of Animal Science, Faculty of Agriculture, Ferdowsi University of MashhadProfessor, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad,Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, IranObjective Salmonella is a Gram-negative, anaerobic, flagellated bacterium of Salmonella type in Enterobacteriaceae family, which is known as a zoonosis infectious agent. In recent years, different types of antibiotics have been used to overcome the Salmonella infection; however, the increasing number of antibiotic-resistant bacteria, the provision of alternative methods seems be necessary. Specific immunoglobulin Y (IgY) originated from egg yolk causes passive immunity in the fetus, and extracting these antibodies from egg yolk is cheaper and more feasible than other antibodies. Numerous studies have shown that the egg yolk IgY has immunogenic function against a wide range of bacterial and viral infections in mammals. The purpose of this study was to produce specific immunoglobulin Y against Salmonella typhimurium and Salmonella enteritidis bacteria in laying hens. Materials and Methods In the current study, six pullets were inoculated and boosted with one milliliter of killed Salmonella typhimurium and Salmonella enteritidis. Injections were done fortnightly until one week before egg production. After breast muscle injection of chickens, the eggs were collected, and the IgY was extracted from yolk by the Polson method with polyethylene glycol 6000. The produced IgY was approved by SDS-PAGE method. The specific indirect ELISA done by specificity test for purified IgY also approved the specific IgY against both Salmonella typhimurium and Salmonella enteritidis. Results The results of SDS - PAGE demonstrated the presence of two protein bands of 27 and 67 kDa representing light and heavy chains of IgY, respectively. The specific indirect ELISA method proved that the specific IgY against Salmonella typhimurium and Salmonella enteritidis was produced in the egg yolk. Conclusions It might be possible to administer the egg yolk containing specific IgY against both Salmonella typhimurium and Salmonella enteritidis, to induce passive immunity for preventing some pathogenic diseases in calves such as diarrhea.https://jab.uk.ac.ir/article_2739_5858828cc41c777fd8228711b73e0447.pdf: laying hensigysalmonellaegg yolk |
| spellingShingle | Shima Eshaghi Mohammadreza Nassiri Mojtaba Tahmoorespour Ali Javadmanesh IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidis مجله بیوتکنولوژی کشاورزی : laying hens igy salmonella egg yolk |
| title | IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidis |
| title_full | IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidis |
| title_fullStr | IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidis |
| title_full_unstemmed | IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidis |
| title_short | IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidis |
| title_sort | igy production in egg yolk against salmonella typhimurium and salmonella enteritidis |
| topic | : laying hens igy salmonella egg yolk |
| url | https://jab.uk.ac.ir/article_2739_5858828cc41c777fd8228711b73e0447.pdf |
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