Identification of novel differentially expressed genes in type 1 diabetes mellitus complications using transcriptomic profiling of UAE patients: a multicenter study
Abstract Type 1 diabetes mellitus (T1DM) is a chronic metabolic disorder that mainly affects children and young adults. It is associated with debilitating and long-life complications. Therefore, understanding the factors that lead to the onset and development of these complications is crucial. To ou...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-18997-w |
| _version_ | 1811202813067264000 |
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| author | Bashair M. Mussa Thenmozhi Venkatachalam Ankita Srivastava Abeer Al-Habshi Elamin Abdelgadir Alaaeldin Bashier Fatheya Al Awadi Khadija Hafidh Rifat Hamoudi Salah Abusnana |
| author_facet | Bashair M. Mussa Thenmozhi Venkatachalam Ankita Srivastava Abeer Al-Habshi Elamin Abdelgadir Alaaeldin Bashier Fatheya Al Awadi Khadija Hafidh Rifat Hamoudi Salah Abusnana |
| author_sort | Bashair M. Mussa |
| collection | DOAJ |
| description | Abstract Type 1 diabetes mellitus (T1DM) is a chronic metabolic disorder that mainly affects children and young adults. It is associated with debilitating and long-life complications. Therefore, understanding the factors that lead to the onset and development of these complications is crucial. To our knowledge this is the first study that attempts to identify the common differentially expressed genes (DEGs) in T1DM complications using whole transcriptomic profiling in United Arab Emirates (UAE) patients. The present multicenter study was conducted in different hospitals in UAE including University Hospital Sharjah, Dubai Hospital and Rashid Hospital. A total of fifty-eight Emirati participants aged above 18 years and with a BMI < 25 kg/m2 were recruited and forty-five of these participants had a confirmed diagnosis of T1DM. Five groups of complications associated with the latter were identified including hyperlipidemia, neuropathy, ketoacidosis, hypothyroidism and polycystic ovary syndrome (PCOS). A comprehensive whole transcriptomic analysis using NGS was conducted. The outcomes of the study revealed the common DEGs between T1DM without complications and T1DM with different complications. The results revealed seven common candidate DEGs, SPINK9, TRDN, PVRL4, MYO3A, PDLIM1, KIAA1614 and GRP were upregulated in T1DM complications with significant increase in expression of SPINK9 (Fold change: 5.28, 3.79, 5.20, 3.79, 5.20) and MYO3A (Fold change: 4.14, 6.11, 2.60, 4.33, 4.49) in hyperlipidemia, neuropathy, ketoacidosis, hypothyroidism and PCOS, respectively. In addition, functional pathways of ion transport, mineral absorption and cytosolic calcium concentration were involved in regulation of candidate upregulated genes related to neuropathy, ketoacidosis and PCOS, respectively. The findings of this study represent a novel reference warranting further studies to shed light on the causative genetic factors that are involved in the onset and development of T1DM complications. |
| first_indexed | 2024-04-12T02:44:57Z |
| format | Article |
| id | doaj.art-0515e1e0c60342d08afef77eae1740dd |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T02:44:57Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0515e1e0c60342d08afef77eae1740dd2022-12-22T03:51:11ZengNature PortfolioScientific Reports2045-23222022-09-0112111310.1038/s41598-022-18997-wIdentification of novel differentially expressed genes in type 1 diabetes mellitus complications using transcriptomic profiling of UAE patients: a multicenter studyBashair M. Mussa0Thenmozhi Venkatachalam1Ankita Srivastava2Abeer Al-Habshi3Elamin Abdelgadir4Alaaeldin Bashier5Fatheya Al Awadi6Khadija Hafidh7Rifat Hamoudi8Salah Abusnana9Basic Medical Science Department, College of Medicine, University of SharjahDepartment of Physiology and Immunology, College of Medicine, Khalifa UniversitySharjah Institute for Medical Research, University of SharjahSharjah Institute for Medical Research, University of SharjahEndocrinology Department, Dubai HospitalEndocrinology Department, Dubai HospitalEndocrinology Department, Dubai HospitalSharjah Institute for Medical Research, University of SharjahSharjah Institute for Medical Research, University of SharjahClinical Science Department, College of Medicine, University of SharjahAbstract Type 1 diabetes mellitus (T1DM) is a chronic metabolic disorder that mainly affects children and young adults. It is associated with debilitating and long-life complications. Therefore, understanding the factors that lead to the onset and development of these complications is crucial. To our knowledge this is the first study that attempts to identify the common differentially expressed genes (DEGs) in T1DM complications using whole transcriptomic profiling in United Arab Emirates (UAE) patients. The present multicenter study was conducted in different hospitals in UAE including University Hospital Sharjah, Dubai Hospital and Rashid Hospital. A total of fifty-eight Emirati participants aged above 18 years and with a BMI < 25 kg/m2 were recruited and forty-five of these participants had a confirmed diagnosis of T1DM. Five groups of complications associated with the latter were identified including hyperlipidemia, neuropathy, ketoacidosis, hypothyroidism and polycystic ovary syndrome (PCOS). A comprehensive whole transcriptomic analysis using NGS was conducted. The outcomes of the study revealed the common DEGs between T1DM without complications and T1DM with different complications. The results revealed seven common candidate DEGs, SPINK9, TRDN, PVRL4, MYO3A, PDLIM1, KIAA1614 and GRP were upregulated in T1DM complications with significant increase in expression of SPINK9 (Fold change: 5.28, 3.79, 5.20, 3.79, 5.20) and MYO3A (Fold change: 4.14, 6.11, 2.60, 4.33, 4.49) in hyperlipidemia, neuropathy, ketoacidosis, hypothyroidism and PCOS, respectively. In addition, functional pathways of ion transport, mineral absorption and cytosolic calcium concentration were involved in regulation of candidate upregulated genes related to neuropathy, ketoacidosis and PCOS, respectively. The findings of this study represent a novel reference warranting further studies to shed light on the causative genetic factors that are involved in the onset and development of T1DM complications.https://doi.org/10.1038/s41598-022-18997-w |
| spellingShingle | Bashair M. Mussa Thenmozhi Venkatachalam Ankita Srivastava Abeer Al-Habshi Elamin Abdelgadir Alaaeldin Bashier Fatheya Al Awadi Khadija Hafidh Rifat Hamoudi Salah Abusnana Identification of novel differentially expressed genes in type 1 diabetes mellitus complications using transcriptomic profiling of UAE patients: a multicenter study Scientific Reports |
| title | Identification of novel differentially expressed genes in type 1 diabetes mellitus complications using transcriptomic profiling of UAE patients: a multicenter study |
| title_full | Identification of novel differentially expressed genes in type 1 diabetes mellitus complications using transcriptomic profiling of UAE patients: a multicenter study |
| title_fullStr | Identification of novel differentially expressed genes in type 1 diabetes mellitus complications using transcriptomic profiling of UAE patients: a multicenter study |
| title_full_unstemmed | Identification of novel differentially expressed genes in type 1 diabetes mellitus complications using transcriptomic profiling of UAE patients: a multicenter study |
| title_short | Identification of novel differentially expressed genes in type 1 diabetes mellitus complications using transcriptomic profiling of UAE patients: a multicenter study |
| title_sort | identification of novel differentially expressed genes in type 1 diabetes mellitus complications using transcriptomic profiling of uae patients a multicenter study |
| url | https://doi.org/10.1038/s41598-022-18997-w |
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