DNA Barcoding of Morphologically Characterized Mosquitoes Belonging to the Genus <i>Mansonia</i> from the Atlantic Forest and Brazilian Savanna
The identification of mosquito species is necessary for determining the entomological components of disease transmission. However, identification can be difficult in species that are morphologically similar. The cytochrome <i>c</i> oxidase subunit I (<i>COI</i>) DNA barcode r...
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MDPI AG
2023-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/2/109 |
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| author | Karin Kirchgatter Lilian de Oliveira Guimarães Eliana Ferreira Monteiro Vanessa Christe Helfstein Juliana Telles-de-Deus Regiane Maria Tironi de Menezes Simone Liuchetta Reginato Carolina Romeiro Fernandes Chagas Vera Lucia Fonseca de Camargo-Neves |
| author_facet | Karin Kirchgatter Lilian de Oliveira Guimarães Eliana Ferreira Monteiro Vanessa Christe Helfstein Juliana Telles-de-Deus Regiane Maria Tironi de Menezes Simone Liuchetta Reginato Carolina Romeiro Fernandes Chagas Vera Lucia Fonseca de Camargo-Neves |
| author_sort | Karin Kirchgatter |
| collection | DOAJ |
| description | The identification of mosquito species is necessary for determining the entomological components of disease transmission. However, identification can be difficult in species that are morphologically similar. The cytochrome <i>c</i> oxidase subunit I (<i>COI</i>) DNA barcode region is considered a valuable and reliable diagnostic tool for mosquito species recognition, including those that belong to species complexes. <i>Mansonia</i> mosquitoes are found in forests near swampy areas. They are nocturnal and are highly attracted to light. Hematophagous adult females exhibit aggressive biting behavior and can become infected with and transmit pathogens during their feeding, including some epizootic viruses and avian malaria. In Brazil, twelve <i>Mansonia</i> species have been reported. In a recent study from the São Paulo Zoo in Brazil, three morphologically distinct species were collected and identified, namely: <i>Mansonia</i> (<i>Mansonia</i>) <i>indubitans</i>, <i>Ma.</i> (<i>Man.</i>) <i>pseudotitillans</i> and <i>Ma.</i> (<i>Man.</i>) <i>titillans.</i> However, confirmation of these species by molecular identification was unsuccessful due to a lack of <i>COI</i> sequences in the GenBank database. Thus, this research aimed to describe the <i>COI</i> DNA barcode sequences of some morphologically characterized <i>Mansonia (Man.)</i> species from Brazil and to determine their utility in delimiting species collected from the Atlantic Forest and Brazilian Savanna. Accordingly, we provide tools for the genetic identification of species that play a significant role in pathogen transmission in wildlife and potentially humans. We show that the delimitation of <i>Mansonia</i> species via five different approaches based on <i>COI</i> DNA sequences (BI, NJ, ASAP, bPTP and GMYC) yield basically the same groups identified by traditional taxonomy, and we provide the identification of specimens that were previously identified only up to the subgenus level. We also provide <i>COI</i> sequences from two <i>Mansonia</i> species that were not previously available in sequence databases, <i>Ma. wilsoni</i> and <i>Ma. pseudotitillans</i>, and thus contribute to the ongoing global effort to standardize DNA barcoding as a molecular means of species identification. |
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| institution | Directory Open Access Journal |
| issn | 2075-4450 |
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| last_indexed | 2024-03-11T08:39:17Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-051c017a83cc4ff9b741bd0a09e5ee5b2023-11-16T21:13:58ZengMDPI AGInsects2075-44502023-01-0114210910.3390/insects14020109DNA Barcoding of Morphologically Characterized Mosquitoes Belonging to the Genus <i>Mansonia</i> from the Atlantic Forest and Brazilian SavannaKarin Kirchgatter0Lilian de Oliveira Guimarães1Eliana Ferreira Monteiro2Vanessa Christe Helfstein3Juliana Telles-de-Deus4Regiane Maria Tironi de Menezes5Simone Liuchetta Reginato6Carolina Romeiro Fernandes Chagas7Vera Lucia Fonseca de Camargo-Neves8Pasteur Institute, São Paulo 01027-000, SP, BrazilPasteur Institute, São Paulo 01027-000, SP, BrazilPasteur Institute, São Paulo 01027-000, SP, BrazilPasteur Institute, São Paulo 01027-000, SP, BrazilPasteur Institute, São Paulo 01027-000, SP, BrazilPasteur Institute, São Paulo 01027-000, SP, BrazilPasteur Institute, São Paulo 01027-000, SP, BrazilNature Research Centre, 08412 Vilnius, LithuaniaPasteur Institute, São Paulo 01027-000, SP, BrazilThe identification of mosquito species is necessary for determining the entomological components of disease transmission. However, identification can be difficult in species that are morphologically similar. The cytochrome <i>c</i> oxidase subunit I (<i>COI</i>) DNA barcode region is considered a valuable and reliable diagnostic tool for mosquito species recognition, including those that belong to species complexes. <i>Mansonia</i> mosquitoes are found in forests near swampy areas. They are nocturnal and are highly attracted to light. Hematophagous adult females exhibit aggressive biting behavior and can become infected with and transmit pathogens during their feeding, including some epizootic viruses and avian malaria. In Brazil, twelve <i>Mansonia</i> species have been reported. In a recent study from the São Paulo Zoo in Brazil, three morphologically distinct species were collected and identified, namely: <i>Mansonia</i> (<i>Mansonia</i>) <i>indubitans</i>, <i>Ma.</i> (<i>Man.</i>) <i>pseudotitillans</i> and <i>Ma.</i> (<i>Man.</i>) <i>titillans.</i> However, confirmation of these species by molecular identification was unsuccessful due to a lack of <i>COI</i> sequences in the GenBank database. Thus, this research aimed to describe the <i>COI</i> DNA barcode sequences of some morphologically characterized <i>Mansonia (Man.)</i> species from Brazil and to determine their utility in delimiting species collected from the Atlantic Forest and Brazilian Savanna. Accordingly, we provide tools for the genetic identification of species that play a significant role in pathogen transmission in wildlife and potentially humans. We show that the delimitation of <i>Mansonia</i> species via five different approaches based on <i>COI</i> DNA sequences (BI, NJ, ASAP, bPTP and GMYC) yield basically the same groups identified by traditional taxonomy, and we provide the identification of specimens that were previously identified only up to the subgenus level. We also provide <i>COI</i> sequences from two <i>Mansonia</i> species that were not previously available in sequence databases, <i>Ma. wilsoni</i> and <i>Ma. pseudotitillans</i>, and thus contribute to the ongoing global effort to standardize DNA barcoding as a molecular means of species identification.https://www.mdpi.com/2075-4450/14/2/109<i>Mansonia</i>BrazilDNA barcode<i>COI</i> sequencesCulicidae |
| spellingShingle | Karin Kirchgatter Lilian de Oliveira Guimarães Eliana Ferreira Monteiro Vanessa Christe Helfstein Juliana Telles-de-Deus Regiane Maria Tironi de Menezes Simone Liuchetta Reginato Carolina Romeiro Fernandes Chagas Vera Lucia Fonseca de Camargo-Neves DNA Barcoding of Morphologically Characterized Mosquitoes Belonging to the Genus <i>Mansonia</i> from the Atlantic Forest and Brazilian Savanna Insects <i>Mansonia</i> Brazil DNA barcode <i>COI</i> sequences Culicidae |
| title | DNA Barcoding of Morphologically Characterized Mosquitoes Belonging to the Genus <i>Mansonia</i> from the Atlantic Forest and Brazilian Savanna |
| title_full | DNA Barcoding of Morphologically Characterized Mosquitoes Belonging to the Genus <i>Mansonia</i> from the Atlantic Forest and Brazilian Savanna |
| title_fullStr | DNA Barcoding of Morphologically Characterized Mosquitoes Belonging to the Genus <i>Mansonia</i> from the Atlantic Forest and Brazilian Savanna |
| title_full_unstemmed | DNA Barcoding of Morphologically Characterized Mosquitoes Belonging to the Genus <i>Mansonia</i> from the Atlantic Forest and Brazilian Savanna |
| title_short | DNA Barcoding of Morphologically Characterized Mosquitoes Belonging to the Genus <i>Mansonia</i> from the Atlantic Forest and Brazilian Savanna |
| title_sort | dna barcoding of morphologically characterized mosquitoes belonging to the genus i mansonia i from the atlantic forest and brazilian savanna |
| topic | <i>Mansonia</i> Brazil DNA barcode <i>COI</i> sequences Culicidae |
| url | https://www.mdpi.com/2075-4450/14/2/109 |
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