Statistical approaches in modeling of the interaction between bacteria and diatom under a dual-species co-cultivation system
This study investigates the co-cultivation system of Nitzschia palea (FACHB-2263) and Pseudomonas chlororaphis (ACCC-19853) in the artificial consortium at different concentrations. The algicidal mode is evaluated using three different full factorial experiments (FFEs) under the following three scen...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364721004055 |
| _version_ | 1819094129275043840 |
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| author | Barathan Balaji Prasath Muhammad Zahir A.M. Elsawah Mohsin Raza Chen Lecong Su Chutian Karen Poon |
| author_facet | Barathan Balaji Prasath Muhammad Zahir A.M. Elsawah Mohsin Raza Chen Lecong Su Chutian Karen Poon |
| author_sort | Barathan Balaji Prasath |
| collection | DOAJ |
| description | This study investigates the co-cultivation system of Nitzschia palea (FACHB-2263) and Pseudomonas chlororaphis (ACCC-19853) in the artificial consortium at different concentrations. The algicidal mode is evaluated using three different full factorial experiments (FFEs) under the following three scenarios: different bacteria culture cell densities against different diatoms; algicidal activities of bacterial culture supernatant against the N. palea in different growth phases, such as lag, exponential and stationary phase; different bacteria and diatom against artificial consortium at different concentrations. For minimizing the effect of random error, three replications of each experimental run of these three FFEs are used. The influence of bacteria on growth in total chlorophyll is analyzed for all of these three replicates FFEs. The results demonstrate that: the P. chlororaphis showed high algicidal activity against N. Palea within 5 days; selected bacteria culture supernatant exhibited strong algicidal activity in the lag phase while the artificial consortium achieved the algal cell lysis at different concentrations of the bacteria to the diatom cells and the diatom to the bacteria cell; and the indoor mesocosms confirmed that P. chlororaphis effectively reduced N. palea cells by over 90% within 5 days. The findings suggest that the artificial consortia algal-bacterial symbiotic system is thus proven to be an effective and promising system for microalgae bloom control treatment algal-bacterial symbiotic system that was extrapolated for novel algae bloom control treatment. |
| first_indexed | 2024-12-21T23:22:30Z |
| format | Article |
| id | doaj.art-051ee44c01b64da0a2276fec2efd30f9 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-21T23:22:30Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-051ee44c01b64da0a2276fec2efd30f92022-12-21T18:46:45ZengElsevierJournal of King Saud University: Science1018-36472022-01-01341101743Statistical approaches in modeling of the interaction between bacteria and diatom under a dual-species co-cultivation systemBarathan Balaji Prasath0Muhammad Zahir1A.M. Elsawah2Mohsin Raza3Chen Lecong4Su Chutian5Karen Poon6Division of Science and Technology, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai 519085, ChinaCentre for Climate Research and Development (CCRD), COMSATS University Islamabad, Park Road, Tarlai Kalan, Islamabad 45550, PakistanDivision of Science and Technology, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai 519085, China; Department of Mathematics, Faculty of Science, Zagazig University, Zagazig 44519, Egypt; Corresponding author at: Division of Science and Technology, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai 519085, China.Centre for Climate Research and Development (CCRD), COMSATS University Islamabad, Park Road, Tarlai Kalan, Islamabad 45550, PakistanDivision of Science and Technology, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai 519085, ChinaDivision of Science and Technology, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai 519085, ChinaDivision of Science and Technology, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai 519085, ChinaThis study investigates the co-cultivation system of Nitzschia palea (FACHB-2263) and Pseudomonas chlororaphis (ACCC-19853) in the artificial consortium at different concentrations. The algicidal mode is evaluated using three different full factorial experiments (FFEs) under the following three scenarios: different bacteria culture cell densities against different diatoms; algicidal activities of bacterial culture supernatant against the N. palea in different growth phases, such as lag, exponential and stationary phase; different bacteria and diatom against artificial consortium at different concentrations. For minimizing the effect of random error, three replications of each experimental run of these three FFEs are used. The influence of bacteria on growth in total chlorophyll is analyzed for all of these three replicates FFEs. The results demonstrate that: the P. chlororaphis showed high algicidal activity against N. Palea within 5 days; selected bacteria culture supernatant exhibited strong algicidal activity in the lag phase while the artificial consortium achieved the algal cell lysis at different concentrations of the bacteria to the diatom cells and the diatom to the bacteria cell; and the indoor mesocosms confirmed that P. chlororaphis effectively reduced N. palea cells by over 90% within 5 days. The findings suggest that the artificial consortia algal-bacterial symbiotic system is thus proven to be an effective and promising system for microalgae bloom control treatment algal-bacterial symbiotic system that was extrapolated for novel algae bloom control treatment.http://www.sciencedirect.com/science/article/pii/S1018364721004055Microalgae-bacteria co-culturingAlgicidal bacteriaPseudomonas chlororaphisNitzschia paleaExperimental design |
| spellingShingle | Barathan Balaji Prasath Muhammad Zahir A.M. Elsawah Mohsin Raza Chen Lecong Su Chutian Karen Poon Statistical approaches in modeling of the interaction between bacteria and diatom under a dual-species co-cultivation system Journal of King Saud University: Science Microalgae-bacteria co-culturing Algicidal bacteria Pseudomonas chlororaphis Nitzschia palea Experimental design |
| title | Statistical approaches in modeling of the interaction between bacteria and diatom under a dual-species co-cultivation system |
| title_full | Statistical approaches in modeling of the interaction between bacteria and diatom under a dual-species co-cultivation system |
| title_fullStr | Statistical approaches in modeling of the interaction between bacteria and diatom under a dual-species co-cultivation system |
| title_full_unstemmed | Statistical approaches in modeling of the interaction between bacteria and diatom under a dual-species co-cultivation system |
| title_short | Statistical approaches in modeling of the interaction between bacteria and diatom under a dual-species co-cultivation system |
| title_sort | statistical approaches in modeling of the interaction between bacteria and diatom under a dual species co cultivation system |
| topic | Microalgae-bacteria co-culturing Algicidal bacteria Pseudomonas chlororaphis Nitzschia palea Experimental design |
| url | http://www.sciencedirect.com/science/article/pii/S1018364721004055 |
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