Positive- and negative-acting regulatory elements contribute to the tissue-specific expression of <it>INNER NO OUTER</it>, a YABBY-type transcription factor gene in Arabidopsis
<p>Abstract</p> <p>Background</p> <p>The <it>INNER NO OUTER</it> (<it>INO</it>) gene, which encodes a YABBY-type transcription factor, specifies and promotes the growth of the outer integument of the ovule in Arabidopsis. <it>INO</it>...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-11-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://www.biomedcentral.com/1471-2229/12/214 |
| _version_ | 1811294349292470272 |
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| author | Simon Marissa K Williams Luis A Brady-Passerini Kristina Brown Ryan H Gasser Charles S |
| author_facet | Simon Marissa K Williams Luis A Brady-Passerini Kristina Brown Ryan H Gasser Charles S |
| author_sort | Simon Marissa K |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The <it>INNER NO OUTER</it> (<it>INO</it>) gene, which encodes a YABBY-type transcription factor, specifies and promotes the growth of the outer integument of the ovule in Arabidopsis. <it>INO</it> expression is limited to the abaxial cell layer of the developing outer integument of the ovule and is regulated by multiple regions of the <it>INO</it> promoter, including POS9, a positive element that when present in quadruplicate can produce low-level expression in the normal <it>INO</it> pattern.</p> <p>Results</p> <p>Significant redundancy in activity between different regions of the <it>INO</it> promoter is demonstrated. For specific regulatory elements, multimerization or the addition of the cauliflower mosaic virus 35S general enhancer was able to activate expression of reporter gene constructs that were otherwise incapable of expression on their own. A new promoter element, POS6, is defined and is shown to include sufficient positive regulatory information to reproduce the endogenous pattern of expression in ovules, but other promoter regions are necessary to fully suppress expression outside of ovules. The full-length <it>INO</it> promoter, but not any of the <it>INO</it> promoter deletions tested, is able to act as an enhancer-blocking insulator to prevent the ectopic activation of expression by the 35S enhancer. Sequence conservation between the promoter regions of <it>Arabidopsis thaliana</it>, <it>Brassica oleracea</it> and <it>Brassica rapa</it> aligns closely with the functional definition of the POS6 and POS9 regions, and with a defined <it>INO</it> minimal promoter. The <it>B</it>. <it>oleracea INO</it> promoter is sufficient to promote a similar pattern and level of reporter gene expression in Arabidopsis to that observed for the Arabidopsis promoter.</p> <p>Conclusions</p> <p>At least two independent regions of the <it>INO</it> promoter contain sufficient regulatory information to direct the specific pattern but not the level of <it>INO</it> gene expression. These regulatory regions act in a partially redundant manner to promote the expression in a specific pattern in the ovule and suppress expression outside of ovules. Establishment of this pattern requires cooperation and competition between multiple positive and negative regulatory elements.</p> |
| first_indexed | 2024-04-13T05:15:56Z |
| format | Article |
| id | doaj.art-051fb07154384973b9d2e7168d23511e |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-13T05:15:56Z |
| publishDate | 2012-11-01 |
| publisher | BMC |
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| spelling | doaj.art-051fb07154384973b9d2e7168d23511e2022-12-22T03:00:54ZengBMCBMC Plant Biology1471-22292012-11-0112121410.1186/1471-2229-12-214Positive- and negative-acting regulatory elements contribute to the tissue-specific expression of <it>INNER NO OUTER</it>, a YABBY-type transcription factor gene in ArabidopsisSimon Marissa KWilliams Luis ABrady-Passerini KristinaBrown Ryan HGasser Charles S<p>Abstract</p> <p>Background</p> <p>The <it>INNER NO OUTER</it> (<it>INO</it>) gene, which encodes a YABBY-type transcription factor, specifies and promotes the growth of the outer integument of the ovule in Arabidopsis. <it>INO</it> expression is limited to the abaxial cell layer of the developing outer integument of the ovule and is regulated by multiple regions of the <it>INO</it> promoter, including POS9, a positive element that when present in quadruplicate can produce low-level expression in the normal <it>INO</it> pattern.</p> <p>Results</p> <p>Significant redundancy in activity between different regions of the <it>INO</it> promoter is demonstrated. For specific regulatory elements, multimerization or the addition of the cauliflower mosaic virus 35S general enhancer was able to activate expression of reporter gene constructs that were otherwise incapable of expression on their own. A new promoter element, POS6, is defined and is shown to include sufficient positive regulatory information to reproduce the endogenous pattern of expression in ovules, but other promoter regions are necessary to fully suppress expression outside of ovules. The full-length <it>INO</it> promoter, but not any of the <it>INO</it> promoter deletions tested, is able to act as an enhancer-blocking insulator to prevent the ectopic activation of expression by the 35S enhancer. Sequence conservation between the promoter regions of <it>Arabidopsis thaliana</it>, <it>Brassica oleracea</it> and <it>Brassica rapa</it> aligns closely with the functional definition of the POS6 and POS9 regions, and with a defined <it>INO</it> minimal promoter. The <it>B</it>. <it>oleracea INO</it> promoter is sufficient to promote a similar pattern and level of reporter gene expression in Arabidopsis to that observed for the Arabidopsis promoter.</p> <p>Conclusions</p> <p>At least two independent regions of the <it>INO</it> promoter contain sufficient regulatory information to direct the specific pattern but not the level of <it>INO</it> gene expression. These regulatory regions act in a partially redundant manner to promote the expression in a specific pattern in the ovule and suppress expression outside of ovules. Establishment of this pattern requires cooperation and competition between multiple positive and negative regulatory elements.</p>http://www.biomedcentral.com/1471-2229/12/214PromoterEnhancerOvuleBrassicaPlant development |
| spellingShingle | Simon Marissa K Williams Luis A Brady-Passerini Kristina Brown Ryan H Gasser Charles S Positive- and negative-acting regulatory elements contribute to the tissue-specific expression of <it>INNER NO OUTER</it>, a YABBY-type transcription factor gene in Arabidopsis BMC Plant Biology Promoter Enhancer Ovule Brassica Plant development |
| title | Positive- and negative-acting regulatory elements contribute to the tissue-specific expression of <it>INNER NO OUTER</it>, a YABBY-type transcription factor gene in Arabidopsis |
| title_full | Positive- and negative-acting regulatory elements contribute to the tissue-specific expression of <it>INNER NO OUTER</it>, a YABBY-type transcription factor gene in Arabidopsis |
| title_fullStr | Positive- and negative-acting regulatory elements contribute to the tissue-specific expression of <it>INNER NO OUTER</it>, a YABBY-type transcription factor gene in Arabidopsis |
| title_full_unstemmed | Positive- and negative-acting regulatory elements contribute to the tissue-specific expression of <it>INNER NO OUTER</it>, a YABBY-type transcription factor gene in Arabidopsis |
| title_short | Positive- and negative-acting regulatory elements contribute to the tissue-specific expression of <it>INNER NO OUTER</it>, a YABBY-type transcription factor gene in Arabidopsis |
| title_sort | positive and negative acting regulatory elements contribute to the tissue specific expression of it inner no outer it a yabby type transcription factor gene in arabidopsis |
| topic | Promoter Enhancer Ovule Brassica Plant development |
| url | http://www.biomedcentral.com/1471-2229/12/214 |
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