Differential Gene Expression Patterns in Blood and Cerebrospinal Fluid of Multiple Sclerosis and Neuro-Behçet Disease
Inflammatory demyelinating disorders of the central nervous system are debilitating conditions of the young adult, here we focus on multiple sclerosis (MS) and neuro-Behçet disease (NBD). MS is an autoimmune disorder of the central nervous system. NBD, a neurological manifestation of an idiopathic c...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.638236/full |
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| author | Olfa Maghrebi Olfa Maghrebi Olfa Maghrebi Mariem Hanachi Mariem Hanachi Khadija Bahrini Khadija Bahrini Mariem Kchaou Mariem Kchaou Cyrine Jeridi Cyrine Jeridi Samir Belal Samir Belal Samia Ben Sassi Samia Ben Sassi Mohamed-Ridha Barbouche Mohamed-Ridha Barbouche Oussama Souiai Oussama Souiai Meriam Belghith Meriam Belghith |
| author_facet | Olfa Maghrebi Olfa Maghrebi Olfa Maghrebi Mariem Hanachi Mariem Hanachi Khadija Bahrini Khadija Bahrini Mariem Kchaou Mariem Kchaou Cyrine Jeridi Cyrine Jeridi Samir Belal Samir Belal Samia Ben Sassi Samia Ben Sassi Mohamed-Ridha Barbouche Mohamed-Ridha Barbouche Oussama Souiai Oussama Souiai Meriam Belghith Meriam Belghith |
| author_sort | Olfa Maghrebi |
| collection | DOAJ |
| description | Inflammatory demyelinating disorders of the central nervous system are debilitating conditions of the young adult, here we focus on multiple sclerosis (MS) and neuro-Behçet disease (NBD). MS is an autoimmune disorder of the central nervous system. NBD, a neurological manifestation of an idiopathic chronic relapsing multisystem inflammatory disease, the behçet disease. The diagnosis of MS and NBD relies on clinical symptoms, magnetic resonance imaging and laboratory tests. At first onset, clinical and imaging similarities between the two disorders may occur, making differential diagnosis challenging and delaying appropriate management. Aiming to identify additional discriminating biomarker patterns, we measured and compared gene expression of a broad panel of selected genes in blood and cerebrospinal fluid (CSF) cells of patients suffering from NBD, MS and non inflammatory neurological disorders (NIND). To reach this aim, bivariate and multivariate analysis were applied. The Principal Analysis Component (PCA) highlighted distinct profiles between NBD, MS, and controls. Transcription factors foxp3 in the blood along with IL-4, IL-10, and IL-17 expressions were the parameters that are the main contributor to the segregation between MS and NBD clustering. Moreover, parameters related to cellular activation and inflammatory cytokines within the CSF clearly differentiate between the two inflammatory diseases and the controls. We proceeded to ROC analysis in order to identify the most distinctive parameters between both inflammatory neurological disorders. The latter analysis suggested that IL-17, CD73 in the blood as well as IL-1β and IL-10 in the CSF were the most discriminating parameters between MS and NBD. We conclude that combined multi-dimensional analysis in blood and CSF suggests distinct mechanisms governing the pathophysiology of these two neuro-inflammatory disorders. |
| first_indexed | 2024-12-17T05:14:10Z |
| format | Article |
| id | doaj.art-0521df8d8f7341bc9b79c851a79678a7 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-17T05:14:10Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-0521df8d8f7341bc9b79c851a79678a72022-12-21T22:02:09ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-02-011210.3389/fgene.2021.638236638236Differential Gene Expression Patterns in Blood and Cerebrospinal Fluid of Multiple Sclerosis and Neuro-Behçet DiseaseOlfa Maghrebi0Olfa Maghrebi1Olfa Maghrebi2Mariem Hanachi3Mariem Hanachi4Khadija Bahrini5Khadija Bahrini6Mariem Kchaou7Mariem Kchaou8Cyrine Jeridi9Cyrine Jeridi10Samir Belal11Samir Belal12Samia Ben Sassi13Samia Ben Sassi14Mohamed-Ridha Barbouche15Mohamed-Ridha Barbouche16Oussama Souiai17Oussama Souiai18Meriam Belghith19Meriam Belghith20Laboratory of Transmission, Control and Immunobiology of Infections, Institut Pasteur de Tunis, Tunis, TunisiaFaculty of Medicine, Tunis El Manar University, Tunis, TunisiaTunis El Manar University, Tunis, TunisiaLaboratory of Bioinformatics, Biomathematics and Biostatistics-LR16IPT09, Institut Pasteur de Tunis, Tunis, TunisiaFaculty of Science of Bizerte, University of Carthage, Jarzouna, TunisiaLaboratory of Transmission, Control and Immunobiology of Infections, Institut Pasteur de Tunis, Tunis, TunisiaTunis El Manar University, Tunis, TunisiaFaculty of Medicine, Tunis El Manar University, Tunis, TunisiaCharles Nicolle Hospital, Tunis, TunisiaFaculty of Medicine, Tunis El Manar University, Tunis, TunisiaDepartment of Neurology, Mongi Ben Hmida National Institute of Neurology, Tunis, TunisiaFaculty of Medicine, Tunis El Manar University, Tunis, TunisiaDepartment of Neurology, Mongi Ben Hmida National Institute of Neurology, Tunis, TunisiaFaculty of Medicine, Tunis El Manar University, Tunis, TunisiaDepartment of Neurology, Mongi Ben Hmida National Institute of Neurology, Tunis, TunisiaLaboratory of Transmission, Control and Immunobiology of Infections, Institut Pasteur de Tunis, Tunis, TunisiaFaculty of Medicine, Tunis El Manar University, Tunis, TunisiaTunis El Manar University, Tunis, TunisiaLaboratory of Bioinformatics, Biomathematics and Biostatistics-LR16IPT09, Institut Pasteur de Tunis, Tunis, TunisiaLaboratory of Transmission, Control and Immunobiology of Infections, Institut Pasteur de Tunis, Tunis, TunisiaTunis El Manar University, Tunis, TunisiaInflammatory demyelinating disorders of the central nervous system are debilitating conditions of the young adult, here we focus on multiple sclerosis (MS) and neuro-Behçet disease (NBD). MS is an autoimmune disorder of the central nervous system. NBD, a neurological manifestation of an idiopathic chronic relapsing multisystem inflammatory disease, the behçet disease. The diagnosis of MS and NBD relies on clinical symptoms, magnetic resonance imaging and laboratory tests. At first onset, clinical and imaging similarities between the two disorders may occur, making differential diagnosis challenging and delaying appropriate management. Aiming to identify additional discriminating biomarker patterns, we measured and compared gene expression of a broad panel of selected genes in blood and cerebrospinal fluid (CSF) cells of patients suffering from NBD, MS and non inflammatory neurological disorders (NIND). To reach this aim, bivariate and multivariate analysis were applied. The Principal Analysis Component (PCA) highlighted distinct profiles between NBD, MS, and controls. Transcription factors foxp3 in the blood along with IL-4, IL-10, and IL-17 expressions were the parameters that are the main contributor to the segregation between MS and NBD clustering. Moreover, parameters related to cellular activation and inflammatory cytokines within the CSF clearly differentiate between the two inflammatory diseases and the controls. We proceeded to ROC analysis in order to identify the most distinctive parameters between both inflammatory neurological disorders. The latter analysis suggested that IL-17, CD73 in the blood as well as IL-1β and IL-10 in the CSF were the most discriminating parameters between MS and NBD. We conclude that combined multi-dimensional analysis in blood and CSF suggests distinct mechanisms governing the pathophysiology of these two neuro-inflammatory disorders.https://www.frontiersin.org/articles/10.3389/fgene.2021.638236/fullmultiple sclerosisneuro-Behçet diseasemultivariate analysiscerebrospinal fluidIL-17CD73 |
| spellingShingle | Olfa Maghrebi Olfa Maghrebi Olfa Maghrebi Mariem Hanachi Mariem Hanachi Khadija Bahrini Khadija Bahrini Mariem Kchaou Mariem Kchaou Cyrine Jeridi Cyrine Jeridi Samir Belal Samir Belal Samia Ben Sassi Samia Ben Sassi Mohamed-Ridha Barbouche Mohamed-Ridha Barbouche Oussama Souiai Oussama Souiai Meriam Belghith Meriam Belghith Differential Gene Expression Patterns in Blood and Cerebrospinal Fluid of Multiple Sclerosis and Neuro-Behçet Disease Frontiers in Genetics multiple sclerosis neuro-Behçet disease multivariate analysis cerebrospinal fluid IL-17 CD73 |
| title | Differential Gene Expression Patterns in Blood and Cerebrospinal Fluid of Multiple Sclerosis and Neuro-Behçet Disease |
| title_full | Differential Gene Expression Patterns in Blood and Cerebrospinal Fluid of Multiple Sclerosis and Neuro-Behçet Disease |
| title_fullStr | Differential Gene Expression Patterns in Blood and Cerebrospinal Fluid of Multiple Sclerosis and Neuro-Behçet Disease |
| title_full_unstemmed | Differential Gene Expression Patterns in Blood and Cerebrospinal Fluid of Multiple Sclerosis and Neuro-Behçet Disease |
| title_short | Differential Gene Expression Patterns in Blood and Cerebrospinal Fluid of Multiple Sclerosis and Neuro-Behçet Disease |
| title_sort | differential gene expression patterns in blood and cerebrospinal fluid of multiple sclerosis and neuro behcet disease |
| topic | multiple sclerosis neuro-Behçet disease multivariate analysis cerebrospinal fluid IL-17 CD73 |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.638236/full |
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