Process Design of Microbiological Chitin Extraction
Chitin extraction from shrimp shells involves two processing steps, these are the deproteination and demineralization process. The aim of this experiment was to compare the order of the chitin extraction process. The first experiment was deproteination of fresh shrimp shells followed by demineraliza...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Microbiology
2011-04-01
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| Series: | Microbiology Indonesia |
| Subjects: | |
| Online Access: | https://jurnal.permi.or.id/index.php/mionline/article/view/133 |
| _version_ | 1819156497741905920 |
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| author | BUDIASIH WAHYUNTARI . JUNIANTO SISWA SETYAHADI |
| author_facet | BUDIASIH WAHYUNTARI . JUNIANTO SISWA SETYAHADI |
| author_sort | BUDIASIH WAHYUNTARI |
| collection | DOAJ |
| description | Chitin extraction from shrimp shells involves two processing steps, these are the deproteination and demineralization process. The aim of this experiment was to compare the order of the chitin extraction process. The first experiment was deproteination of fresh shrimp shells followed by demineralization process and the second one was demineralization of fresh shrimp shells followed by deproteination. Bacillus licheniformis F11.1, a proteolytic producing bacterium, was used for the deproteination process. Lactobacillus acidophilus FNCC116, a lactic acid bacterium, was used for the demineralization process. The deproteination was done in a 1 liter fermenter jar at 55 ºC, 250 rpm and 2.5 vvm aeration for 60 h. The demineralization was done in the same size fermenter at 30 ºC and 50 rpm agitation for 48 h. The experimental results showed that demineralization followed by the deproteination process resulted in a better chitin yield than when the process was conducted in the opposite order. The first process reduced 47.37% protein and 50.23% ash, whereas the second process reduced 79.61% protein and 88.65% ash |
| first_indexed | 2024-12-22T15:53:49Z |
| format | Article |
| id | doaj.art-0523e6382489496bb42cc7713f5eeeaf |
| institution | Directory Open Access Journal |
| issn | 1978-3477 2087-8575 |
| language | English |
| last_indexed | 2024-12-22T15:53:49Z |
| publishDate | 2011-04-01 |
| publisher | Indonesian Society for Microbiology |
| record_format | Article |
| series | Microbiology Indonesia |
| spelling | doaj.art-0523e6382489496bb42cc7713f5eeeaf2022-12-21T18:20:50ZengIndonesian Society for MicrobiologyMicrobiology Indonesia1978-34772087-85752011-04-015110.5454/mi.5.1.7Process Design of Microbiological Chitin ExtractionBUDIASIH WAHYUNTARI0. JUNIANTO1SISWA SETYAHADI2Center for Bioindustrial Technology, Laboratoria Pengembangnan Teknologi Agro-Biomedika, Badan Pengkajian dan Penerapan Teknologi, Pusat Penelitian Ilmu Pengetahuan dan Teknologi,Faculty of Fishery and Marine Sciences, Universitas PadjajaranCenter for Bioindustrial Technology, Laboratoria Pengembangnan Teknologi Agro-Biomedika, Badan Pengkajian dan Penerapan Teknologi, Pusat Penelitian Ilmu Pengetahuan dan Teknologi,Chitin extraction from shrimp shells involves two processing steps, these are the deproteination and demineralization process. The aim of this experiment was to compare the order of the chitin extraction process. The first experiment was deproteination of fresh shrimp shells followed by demineralization process and the second one was demineralization of fresh shrimp shells followed by deproteination. Bacillus licheniformis F11.1, a proteolytic producing bacterium, was used for the deproteination process. Lactobacillus acidophilus FNCC116, a lactic acid bacterium, was used for the demineralization process. The deproteination was done in a 1 liter fermenter jar at 55 ºC, 250 rpm and 2.5 vvm aeration for 60 h. The demineralization was done in the same size fermenter at 30 ºC and 50 rpm agitation for 48 h. The experimental results showed that demineralization followed by the deproteination process resulted in a better chitin yield than when the process was conducted in the opposite order. The first process reduced 47.37% protein and 50.23% ash, whereas the second process reduced 79.61% protein and 88.65% ashhttps://jurnal.permi.or.id/index.php/mionline/article/view/133microbiological chitin extractiondeproteinationdemineralization |
| spellingShingle | BUDIASIH WAHYUNTARI . JUNIANTO SISWA SETYAHADI Process Design of Microbiological Chitin Extraction Microbiology Indonesia microbiological chitin extraction deproteination demineralization |
| title | Process Design of Microbiological Chitin Extraction |
| title_full | Process Design of Microbiological Chitin Extraction |
| title_fullStr | Process Design of Microbiological Chitin Extraction |
| title_full_unstemmed | Process Design of Microbiological Chitin Extraction |
| title_short | Process Design of Microbiological Chitin Extraction |
| title_sort | process design of microbiological chitin extraction |
| topic | microbiological chitin extraction deproteination demineralization |
| url | https://jurnal.permi.or.id/index.php/mionline/article/view/133 |
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