Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper <i>Sogatella furcifera</i> (Homoptera: Delphacidae)
The white-backed planthopper (WBPH) <i>Sogatella furcifera</i> is one of the most harmful pests of rice in Southeast Asia. The fat body of WBPH harbors intracellular yeast-like symbionts (YLS). YLS are vertically transmitted to WBPH offspring by transovarial infection. YLS play an import...
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MDPI AG
2020-03-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/4/209 |
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| author | Kun Pang Shengzhang Dong Peiying Hao Tongtong Chen Xinlong Wang Xiaoping Yu Huafeng Lin |
| author_facet | Kun Pang Shengzhang Dong Peiying Hao Tongtong Chen Xinlong Wang Xiaoping Yu Huafeng Lin |
| author_sort | Kun Pang |
| collection | DOAJ |
| description | The white-backed planthopper (WBPH) <i>Sogatella furcifera</i> is one of the most harmful pests of rice in Southeast Asia. The fat body of WBPH harbors intracellular yeast-like symbionts (YLS). YLS are vertically transmitted to WBPH offspring by transovarial infection. YLS play an important role in the WBPH life cycle. YLS diversity and function have been extensively studied in the brown planthopper (BPH) and small brown planthopper but not in WBPH, even though a novel strategy for controlling the BPH based on suppressing YLS has been proposed. Here, using denaturing gradient gel electrophoresis, we identified 12 unique fungal sequences among YLS of WBPH, and five of them represented uncultured fungi. We then fed WBPH with rice plants treated with different fungicides [70% propineb wettable powder (WP) (PR), 70% propamocarb hydrochloride aqueous solution (AS) (PH), 25% trifloxystrobin and 50% tebuconazole water-dispersible granules (WG) (TT), 40% pyrimethanil suspension concentrate (SC) (PY), and 50% iprodione SC (IP)] and evaluated their effects on YLS abundance and WBPH survival rate. Both YLS abundance and adult WBPH survival rate were significantly decreased upon feeding fungicide-treated rice plants, and exposure to 50% IP resulted in the strongest reduction. The abundance of two Sf-YLS species (<i>Ascomycetes</i> symbiotes and <i>Cla</i>-like symbiotes) was significantly reduced upon exposure to 50% IP. The counts of <i>Ascomycetes</i> symbiotes, the most abundant YLS species, were also suppressed by the other fungicides tested. In conclusion, 50% IP was the most effective fungicide, reducing YLS abundance and WBPH survival rate under controlled conditions, suggesting its potential use to control WBPH. |
| first_indexed | 2024-03-11T10:11:27Z |
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| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T10:11:27Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-0523e71500874b8e8905019079cc5e482023-11-16T14:29:15ZengMDPI AGInsects2075-44502020-03-0111420910.3390/insects11040209Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper <i>Sogatella furcifera</i> (Homoptera: Delphacidae)Kun Pang0Shengzhang Dong1Peiying Hao2Tongtong Chen3Xinlong Wang4Xiaoping Yu5Huafeng Lin6School of Plant Protection, Anhui Agricultural University, Hefei 230036, ChinaW. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USAZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaSchool of Plant Protection, Anhui Agricultural University, Hefei 230036, ChinaThe white-backed planthopper (WBPH) <i>Sogatella furcifera</i> is one of the most harmful pests of rice in Southeast Asia. The fat body of WBPH harbors intracellular yeast-like symbionts (YLS). YLS are vertically transmitted to WBPH offspring by transovarial infection. YLS play an important role in the WBPH life cycle. YLS diversity and function have been extensively studied in the brown planthopper (BPH) and small brown planthopper but not in WBPH, even though a novel strategy for controlling the BPH based on suppressing YLS has been proposed. Here, using denaturing gradient gel electrophoresis, we identified 12 unique fungal sequences among YLS of WBPH, and five of them represented uncultured fungi. We then fed WBPH with rice plants treated with different fungicides [70% propineb wettable powder (WP) (PR), 70% propamocarb hydrochloride aqueous solution (AS) (PH), 25% trifloxystrobin and 50% tebuconazole water-dispersible granules (WG) (TT), 40% pyrimethanil suspension concentrate (SC) (PY), and 50% iprodione SC (IP)] and evaluated their effects on YLS abundance and WBPH survival rate. Both YLS abundance and adult WBPH survival rate were significantly decreased upon feeding fungicide-treated rice plants, and exposure to 50% IP resulted in the strongest reduction. The abundance of two Sf-YLS species (<i>Ascomycetes</i> symbiotes and <i>Cla</i>-like symbiotes) was significantly reduced upon exposure to 50% IP. The counts of <i>Ascomycetes</i> symbiotes, the most abundant YLS species, were also suppressed by the other fungicides tested. In conclusion, 50% IP was the most effective fungicide, reducing YLS abundance and WBPH survival rate under controlled conditions, suggesting its potential use to control WBPH.https://www.mdpi.com/2075-4450/11/4/209YLS diversity, DGGE<i>Ascomycetes</i> symbioteseffective strategysynergistic effects |
| spellingShingle | Kun Pang Shengzhang Dong Peiying Hao Tongtong Chen Xinlong Wang Xiaoping Yu Huafeng Lin Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper <i>Sogatella furcifera</i> (Homoptera: Delphacidae) Insects YLS diversity, DGGE <i>Ascomycetes</i> symbiotes effective strategy synergistic effects |
| title | Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper <i>Sogatella furcifera</i> (Homoptera: Delphacidae) |
| title_full | Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper <i>Sogatella furcifera</i> (Homoptera: Delphacidae) |
| title_fullStr | Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper <i>Sogatella furcifera</i> (Homoptera: Delphacidae) |
| title_full_unstemmed | Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper <i>Sogatella furcifera</i> (Homoptera: Delphacidae) |
| title_short | Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper <i>Sogatella furcifera</i> (Homoptera: Delphacidae) |
| title_sort | fungicides reduce the abundance of yeast like symbionts and survival of white backed planthopper i sogatella furcifera i homoptera delphacidae |
| topic | YLS diversity, DGGE <i>Ascomycetes</i> symbiotes effective strategy synergistic effects |
| url | https://www.mdpi.com/2075-4450/11/4/209 |
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