Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains
Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 Aids inpatients and 10 environment and animals specimens identified among 53 myco...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2005-11-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000700012 |
| _version_ | 1827839681616674816 |
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| author | Patrícia Carvalho de Sequeira Leila de Souza Fonseca Marlei Gomes da Silva Maria Helena Féres Saad |
| author_facet | Patrícia Carvalho de Sequeira Leila de Souza Fonseca Marlei Gomes da Silva Maria Helena Féres Saad |
| author_sort | Patrícia Carvalho de Sequeira |
| collection | DOAJ |
| description | Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 Aids inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns except for one band. |
| first_indexed | 2024-03-12T07:23:50Z |
| format | Article |
| id | doaj.art-0525b9e9f5d84d639b3c98415ce27404 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:23:50Z |
| publishDate | 2005-11-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-0525b9e9f5d84d639b3c98415ce274042023-09-02T22:14:54ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602005-11-01100774374810.1590/S0074-02762005000700012Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strainsPatrícia Carvalho de SequeiraLeila de Souza FonsecaMarlei Gomes da SilvaMaria Helena Féres SaadSimple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 Aids inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns except for one band.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000700012IS1254restriction fragment length polymorphismMycobacterium aviumMaDRE- polymerase chain reactionnontuberculous mycobacteriaAids |
| spellingShingle | Patrícia Carvalho de Sequeira Leila de Souza Fonseca Marlei Gomes da Silva Maria Helena Féres Saad Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains Memorias do Instituto Oswaldo Cruz IS1254 restriction fragment length polymorphism Mycobacterium avium MaDRE- polymerase chain reaction nontuberculous mycobacteria Aids |
| title | Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains |
| title_full | Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains |
| title_fullStr | Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains |
| title_full_unstemmed | Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains |
| title_short | Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains |
| title_sort | mycobacterium avium restriction fragment lenght polymorphism is is1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in brazilian strains |
| topic | IS1254 restriction fragment length polymorphism Mycobacterium avium MaDRE- polymerase chain reaction nontuberculous mycobacteria Aids |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000700012 |
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