Optimization of Bioethanol Production from Coffee Mucilage

A response surface methodology with 2k full factorial design was applied to obtain optimum conditions for bioethanol production using coffee mucilage (CM) as the substrate and Saccharomyces cerevisiae NRRL Y-2034 as the inoculum. CM is an agro-industrial residue mainly composed of simple sugars; the...

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Main Authors: Antonio De León-Rodríguez, Sergio Saldaña-Trinidad, Sebastian Pathiyamattom Joseph, Bianca Yadira Pérez-Sariñana
Format: Article
Language:English
Published: North Carolina State University 2015-05-01
Series:BioResources
Subjects:
Online Access:http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_4326_Perez_Sarinana_Optimization_Bioethanol_Production
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author Antonio De León-Rodríguez
Sergio Saldaña-Trinidad
Sebastian Pathiyamattom Joseph
Bianca Yadira Pérez-Sariñana
author_facet Antonio De León-Rodríguez
Sergio Saldaña-Trinidad
Sebastian Pathiyamattom Joseph
Bianca Yadira Pérez-Sariñana
author_sort Antonio De León-Rodríguez
collection DOAJ
description A response surface methodology with 2k full factorial design was applied to obtain optimum conditions for bioethanol production using coffee mucilage (CM) as the substrate and Saccharomyces cerevisiae NRRL Y-2034 as the inoculum. CM is an agro-industrial residue mainly composed of simple sugars; the product yield and productivity process were analyzed with respect to the fermentation, pH, temperature, and the initial sugar concentration. Employing the following predicted optimum operational conditions attained the highest bioethanol production: pH 5.1, temperature 32 °C, and initial sugar concentration 61.8 g/L. The estimated bioethanol production was 15.02 g/L, and the experimental production was 16.29 g/L ± 0.39 g/L, with a bioethanol yield of 0.27 g/L and a productivity process of 0.34 g/Lh. Glycerol was the predominant byproduct of the fermentative metabolism of S. cerevisiae. The response surface methodology was successfully employed to optimize CM fermentation. In the fermentative processes with yeast, optimizing the conditions of the culture medium is needed to fully exploit the potential of the strains and maximize the production of bioethanol.
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spelling doaj.art-0526dde2676548dba7aec10843c25f622022-12-22T00:47:13ZengNorth Carolina State UniversityBioResources1930-21261930-21262015-05-011034326433810.15376/biores.10.3.4326-4338Optimization of Bioethanol Production from Coffee MucilageAntonio De León-Rodríguez0Sergio Saldaña-Trinidad1Sebastian Pathiyamattom Joseph2Bianca Yadira Pérez-Sariñana3Instituto Potosino de Investigación Científica y Tecnológica; MexicoUniversidad Politécnica de Chiapas; MexicoInstituto de Energías Renovables; MexicoInstituto de Energías Renovables; MexicoA response surface methodology with 2k full factorial design was applied to obtain optimum conditions for bioethanol production using coffee mucilage (CM) as the substrate and Saccharomyces cerevisiae NRRL Y-2034 as the inoculum. CM is an agro-industrial residue mainly composed of simple sugars; the product yield and productivity process were analyzed with respect to the fermentation, pH, temperature, and the initial sugar concentration. Employing the following predicted optimum operational conditions attained the highest bioethanol production: pH 5.1, temperature 32 °C, and initial sugar concentration 61.8 g/L. The estimated bioethanol production was 15.02 g/L, and the experimental production was 16.29 g/L ± 0.39 g/L, with a bioethanol yield of 0.27 g/L and a productivity process of 0.34 g/Lh. Glycerol was the predominant byproduct of the fermentative metabolism of S. cerevisiae. The response surface methodology was successfully employed to optimize CM fermentation. In the fermentative processes with yeast, optimizing the conditions of the culture medium is needed to fully exploit the potential of the strains and maximize the production of bioethanol.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_4326_Perez_Sarinana_Optimization_Bioethanol_ProductionFementationOptimizationBioethanol production
spellingShingle Antonio De León-Rodríguez
Sergio Saldaña-Trinidad
Sebastian Pathiyamattom Joseph
Bianca Yadira Pérez-Sariñana
Optimization of Bioethanol Production from Coffee Mucilage
BioResources
Fementation
Optimization
Bioethanol production
title Optimization of Bioethanol Production from Coffee Mucilage
title_full Optimization of Bioethanol Production from Coffee Mucilage
title_fullStr Optimization of Bioethanol Production from Coffee Mucilage
title_full_unstemmed Optimization of Bioethanol Production from Coffee Mucilage
title_short Optimization of Bioethanol Production from Coffee Mucilage
title_sort optimization of bioethanol production from coffee mucilage
topic Fementation
Optimization
Bioethanol production
url http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_4326_Perez_Sarinana_Optimization_Bioethanol_Production
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AT biancayadiraperezsarinana optimizationofbioethanolproductionfromcoffeemucilage