circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis
Abstract Objective Osteoporosis is the imbalance in bone homeostasis between osteoblasts and osteoclasts. In this study, we investigated the effects of the circ_0029463/miR-134-5p/Rab27a axis on RANKL-induced osteoclast differentiation. Methods RT-qPCR and western blotting were used to detect the ex...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-024-04610-5 |
| _version_ | 1797273787577663488 |
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| author | Lian Tang Lin Yuan Jiyuan Yan Jianhua Ge Zhi Lian Zhong Li |
| author_facet | Lian Tang Lin Yuan Jiyuan Yan Jianhua Ge Zhi Lian Zhong Li |
| author_sort | Lian Tang |
| collection | DOAJ |
| description | Abstract Objective Osteoporosis is the imbalance in bone homeostasis between osteoblasts and osteoclasts. In this study, we investigated the effects of the circ_0029463/miR-134-5p/Rab27a axis on RANKL-induced osteoclast differentiation. Methods RT-qPCR and western blotting were used to detect the expression of circ_0029463, miR-134-5p, and Rab27a in tissues from patients with osteoporosis and in RANKL-induced osteoclasts. Osteoclast differentiation was verified by TRAP staining. Osteoclast biomarkers, including NFATc1, TRAP, and CTSK, were measured. The target and regulatory relationships between circ_0029463, miR-134-5p, and the Rab27a axis were verified using RIP, dual-luciferase reporter gene, and RNA pull-down assays. Results Elevated expression of circ_0029463 and Rab27a and decreased miR-134-5p expression were observed in the tissues of patients with osteoporosis, and a similar expression pattern was observed in RANKL-induced osteoclasts. Suppression of circ_0029463 expression or miR-134-5p overexpression curbed RANKL-induced osteoclast differentiation, whereas such an effect was abolished by Rab27 overexpression. circ_0029463 sponges miR-134-5p to induce Rab27a expression. Conclusion circ_0029463 sponges miR-134-5p to abolish its suppressive effect of miR-134-5p on Rab27a expression, thereby promoting osteoclast differentiation. |
| first_indexed | 2024-03-07T14:49:12Z |
| format | Article |
| id | doaj.art-05289a1f6141426992a49b892f5f44f9 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-03-07T14:49:12Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-05289a1f6141426992a49b892f5f44f92024-03-05T19:46:13ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2024-02-0119111110.1186/s13018-024-04610-5circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axisLian Tang0Lin Yuan1Jiyuan Yan2Jianhua Ge3Zhi Lian4Zhong Li5Department of Orthopedics, Affiliated Hospital of Southwest Medical UniversityDepartment of Clinical Skills Center, Affiliated Hospital of Southwest Medical UniversityDepartment of Orthopedics, Affiliated Hospital of Southwest Medical UniversityDepartment of Orthopedics, Affiliated Hospital of Southwest Medical UniversitySouthwest Medical UniversityDepartment of Orthopedics, Affiliated Hospital of Southwest Medical UniversityAbstract Objective Osteoporosis is the imbalance in bone homeostasis between osteoblasts and osteoclasts. In this study, we investigated the effects of the circ_0029463/miR-134-5p/Rab27a axis on RANKL-induced osteoclast differentiation. Methods RT-qPCR and western blotting were used to detect the expression of circ_0029463, miR-134-5p, and Rab27a in tissues from patients with osteoporosis and in RANKL-induced osteoclasts. Osteoclast differentiation was verified by TRAP staining. Osteoclast biomarkers, including NFATc1, TRAP, and CTSK, were measured. The target and regulatory relationships between circ_0029463, miR-134-5p, and the Rab27a axis were verified using RIP, dual-luciferase reporter gene, and RNA pull-down assays. Results Elevated expression of circ_0029463 and Rab27a and decreased miR-134-5p expression were observed in the tissues of patients with osteoporosis, and a similar expression pattern was observed in RANKL-induced osteoclasts. Suppression of circ_0029463 expression or miR-134-5p overexpression curbed RANKL-induced osteoclast differentiation, whereas such an effect was abolished by Rab27 overexpression. circ_0029463 sponges miR-134-5p to induce Rab27a expression. Conclusion circ_0029463 sponges miR-134-5p to abolish its suppressive effect of miR-134-5p on Rab27a expression, thereby promoting osteoclast differentiation.https://doi.org/10.1186/s13018-024-04610-5circ_0029463miR-134-5pRab27aOsteoclast differentiation |
| spellingShingle | Lian Tang Lin Yuan Jiyuan Yan Jianhua Ge Zhi Lian Zhong Li circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis Journal of Orthopaedic Surgery and Research circ_0029463 miR-134-5p Rab27a Osteoclast differentiation |
| title | circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis |
| title_full | circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis |
| title_fullStr | circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis |
| title_full_unstemmed | circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis |
| title_short | circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis |
| title_sort | circ 0029463 promotes osteoclast differentiation by mediating mir 134 5p rab27a axis |
| topic | circ_0029463 miR-134-5p Rab27a Osteoclast differentiation |
| url | https://doi.org/10.1186/s13018-024-04610-5 |
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