Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets
Summary: Here, we present a cost-effective protocol to differentiate bovine fibro-adipogenic progenitors in a thin hydrogel sheet adherent to 96-well plates. We describe steps for the embedding and culturing of cells in alginate sheets, culture maintenance, and analysis. Compared to alternative thre...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723001016 |
| _version_ | 1811154688902430720 |
|---|---|
| author | Joseph J. Caponi Jane Shmushkis Hanna Geissler Mark J. Post Laura Jackisch Eva M. Mall |
| author_facet | Joseph J. Caponi Jane Shmushkis Hanna Geissler Mark J. Post Laura Jackisch Eva M. Mall |
| author_sort | Joseph J. Caponi |
| collection | DOAJ |
| description | Summary: Here, we present a cost-effective protocol to differentiate bovine fibro-adipogenic progenitors in a thin hydrogel sheet adherent to 96-well plates. We describe steps for the embedding and culturing of cells in alginate sheets, culture maintenance, and analysis. Compared to alternative three-dimensional (3D) models such as hydrogel-based microfibers, this approach simplifies automation while retaining efficient maturation of adipocytes. Embedded cells are still subjected to a 3D environment, but the sheets can be handled and analyzed like two-dimensional cultures. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T04:20:14Z |
| format | Article |
| id | doaj.art-0528f3c5ce5d446db0c8364ad0443bdf |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T04:20:14Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-0528f3c5ce5d446db0c8364ad0443bdf2023-03-11T04:20:32ZengElsevierSTAR Protocols2666-16672023-06-0142102143Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheetsJoseph J. Caponi0Jane Shmushkis1Hanna Geissler2Mark J. Post3Laura Jackisch4Eva M. Mall5Mosa Meat B.V., Maastricht, the NetherlandsMosa Meat B.V., Maastricht, the NetherlandsMosa Meat B.V., Maastricht, the NetherlandsMosa Meat B.V., Maastricht, the Netherlands; Department of Physiology, Maastricht University, Maastricht, the NetherlandsMosa Meat B.V., Maastricht, the NetherlandsMosa Meat B.V., Maastricht, the Netherlands; Corresponding authorSummary: Here, we present a cost-effective protocol to differentiate bovine fibro-adipogenic progenitors in a thin hydrogel sheet adherent to 96-well plates. We describe steps for the embedding and culturing of cells in alginate sheets, culture maintenance, and analysis. Compared to alternative three-dimensional (3D) models such as hydrogel-based microfibers, this approach simplifies automation while retaining efficient maturation of adipocytes. Embedded cells are still subjected to a 3D environment, but the sheets can be handled and analyzed like two-dimensional cultures. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723001016Cell BiologyCell CultureCell IsolationCell-based AssaysHigh-throughput ScreeningMicroscopy |
| spellingShingle | Joseph J. Caponi Jane Shmushkis Hanna Geissler Mark J. Post Laura Jackisch Eva M. Mall Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets STAR Protocols Cell Biology Cell Culture Cell Isolation Cell-based Assays High-throughput Screening Microscopy |
| title | Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets |
| title_full | Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets |
| title_fullStr | Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets |
| title_full_unstemmed | Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets |
| title_short | Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets |
| title_sort | protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets |
| topic | Cell Biology Cell Culture Cell Isolation Cell-based Assays High-throughput Screening Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723001016 |
| work_keys_str_mv | AT josephjcaponi protocolfordifferentiationofbovineadipogenicprogenitorcellsembeddedinalginatesheets AT janeshmushkis protocolfordifferentiationofbovineadipogenicprogenitorcellsembeddedinalginatesheets AT hannageissler protocolfordifferentiationofbovineadipogenicprogenitorcellsembeddedinalginatesheets AT markjpost protocolfordifferentiationofbovineadipogenicprogenitorcellsembeddedinalginatesheets AT laurajackisch protocolfordifferentiationofbovineadipogenicprogenitorcellsembeddedinalginatesheets AT evammall protocolfordifferentiationofbovineadipogenicprogenitorcellsembeddedinalginatesheets |