Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets
Summary: Here, we present a cost-effective protocol to differentiate bovine fibro-adipogenic progenitors in a thin hydrogel sheet adherent to 96-well plates. We describe steps for the embedding and culturing of cells in alginate sheets, culture maintenance, and analysis. Compared to alternative thre...
Main Authors: | Joseph J. Caponi, Jane Shmushkis, Hanna Geissler, Mark J. Post, Laura Jackisch, Eva M. Mall |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2023-06-01
|
Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723001016 |
Similar Items
-
Protocol to identify small molecules promoting rat and mouse cardiomyocyte proliferation based on the FUCCI and MADM reporters
by: Lixia Zheng, et al.
Published: (2022-12-01) -
Isolation, culture, and use of primary murine myoblasts in small-molecule screens
by: Yue Qu, et al.
Published: (2023-06-01) -
Protocol for isolating human BCAM-positive corneal progenitor cells by flow cytometry and cell sorting
by: Yuzuru Sasamoto, et al.
Published: (2023-09-01) -
Prospective isolation of neural stem and progenitor cells from the developing human brain
by: Daniel Dan Liu, et al.
Published: (2023-12-01) -
Fluorescence-activated cell sorting and phenotypic characterization of human fibro-adipogenic progenitors
by: Tine Borum Billeskov, et al.
Published: (2023-03-01)