Nucleic acid binding and other biomedical properties of artificial oligolysines
Giovanni N Roviello,1 Caterina Vicidomini,1 Vincenzo Costanzo,1 Valentina Roviello2 1CNR Istituto di Biostrutture e Bioimmagini, Via Mezzocannone site and Headquarters, 2Centro Regionale di Competenza (CRdC) Tecnologie, Via Nuova Agnano, Napoli, Italy Abstract: In the present study, we report the in...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2016-11-01
|
| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/nucleic-acid-binding-and-other-biomedical-properties-of-artificial-oli-peer-reviewed-article-IJN |
| _version_ | 1819202448531652608 |
|---|---|
| author | Roviello GN Vicidomini C Costanzo V Roviello V |
| author_facet | Roviello GN Vicidomini C Costanzo V Roviello V |
| author_sort | Roviello GN |
| collection | DOAJ |
| description | Giovanni N Roviello,1 Caterina Vicidomini,1 Vincenzo Costanzo,1 Valentina Roviello2 1CNR Istituto di Biostrutture e Bioimmagini, Via Mezzocannone site and Headquarters, 2Centro Regionale di Competenza (CRdC) Tecnologie, Via Nuova Agnano, Napoli, Italy Abstract: In the present study, we report the interaction of an artificial oligolysine (referred to as AOL) realized in our laboratory with targets of biomedical importance. These included polyinosinic acid (poly rI) and its complex with polycytidylic acid (poly I:C), RNAs with well-known interferon-inducing ability, and double-stranded (ds) DNA. The ability of the peptide to bind both single-stranded poly rI and ds poly I:C RNAs emerged from our circular dichroism (CD) and ultraviolet (UV) studies. In addition, we found that AOL forms complexes with dsDNA, as shown by spectroscopic binding assays and UV thermal denaturation experiments. These findings are encouraging for the possible use of AOL in biomedicine for nucleic acid targeting and oligonucleotide condensation, with the latter being a key step preceding their clinical application. Moreover, we tested the ability of AOL to bind to proteins, using serum albumin as a model protein. We demonstrated the oligolysine–protein binding by CD experiments which suggested that AOL, positively charged under physiological conditions, binds to the protein regions rich in anionic residues. Finally, the morphology characterization of the solid oligolysine, performed by scanning electron microscopy, showed different crystal forms including cubic-shaped crystals confirming the high purity of AOL. Keywords: nucleic acid binding, polyinosinic acid, double-stranded nucleic acids, oligolysine, circular dichroism |
| first_indexed | 2024-12-23T04:04:11Z |
| format | Article |
| id | doaj.art-052bdc7c2d7e426f8cd229891315c9bf |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-23T04:04:11Z |
| publishDate | 2016-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-052bdc7c2d7e426f8cd229891315c9bf2022-12-21T18:00:39ZengDove Medical PressInternational Journal of Nanomedicine1178-20132016-11-01Volume 115897590429967Nucleic acid binding and other biomedical properties of artificial oligolysinesRoviello GNVicidomini CCostanzo VRoviello VGiovanni N Roviello,1 Caterina Vicidomini,1 Vincenzo Costanzo,1 Valentina Roviello2 1CNR Istituto di Biostrutture e Bioimmagini, Via Mezzocannone site and Headquarters, 2Centro Regionale di Competenza (CRdC) Tecnologie, Via Nuova Agnano, Napoli, Italy Abstract: In the present study, we report the interaction of an artificial oligolysine (referred to as AOL) realized in our laboratory with targets of biomedical importance. These included polyinosinic acid (poly rI) and its complex with polycytidylic acid (poly I:C), RNAs with well-known interferon-inducing ability, and double-stranded (ds) DNA. The ability of the peptide to bind both single-stranded poly rI and ds poly I:C RNAs emerged from our circular dichroism (CD) and ultraviolet (UV) studies. In addition, we found that AOL forms complexes with dsDNA, as shown by spectroscopic binding assays and UV thermal denaturation experiments. These findings are encouraging for the possible use of AOL in biomedicine for nucleic acid targeting and oligonucleotide condensation, with the latter being a key step preceding their clinical application. Moreover, we tested the ability of AOL to bind to proteins, using serum albumin as a model protein. We demonstrated the oligolysine–protein binding by CD experiments which suggested that AOL, positively charged under physiological conditions, binds to the protein regions rich in anionic residues. Finally, the morphology characterization of the solid oligolysine, performed by scanning electron microscopy, showed different crystal forms including cubic-shaped crystals confirming the high purity of AOL. Keywords: nucleic acid binding, polyinosinic acid, double-stranded nucleic acids, oligolysine, circular dichroismhttps://www.dovepress.com/nucleic-acid-binding-and-other-biomedical-properties-of-artificial-oli-peer-reviewed-article-IJNnucleic acid bindingpolyinosinic aciddouble-stranded nucleic acidsoligolysinecircular dichroism |
| spellingShingle | Roviello GN Vicidomini C Costanzo V Roviello V Nucleic acid binding and other biomedical properties of artificial oligolysines International Journal of Nanomedicine nucleic acid binding polyinosinic acid double-stranded nucleic acids oligolysine circular dichroism |
| title | Nucleic acid binding and other biomedical properties of artificial oligolysines |
| title_full | Nucleic acid binding and other biomedical properties of artificial oligolysines |
| title_fullStr | Nucleic acid binding and other biomedical properties of artificial oligolysines |
| title_full_unstemmed | Nucleic acid binding and other biomedical properties of artificial oligolysines |
| title_short | Nucleic acid binding and other biomedical properties of artificial oligolysines |
| title_sort | nucleic acid binding and other biomedical properties of artificial oligolysines |
| topic | nucleic acid binding polyinosinic acid double-stranded nucleic acids oligolysine circular dichroism |
| url | https://www.dovepress.com/nucleic-acid-binding-and-other-biomedical-properties-of-artificial-oli-peer-reviewed-article-IJN |
| work_keys_str_mv | AT roviellogn nucleicacidbindingandotherbiomedicalpropertiesofartificialoligolysines AT vicidominic nucleicacidbindingandotherbiomedicalpropertiesofartificialoligolysines AT costanzov nucleicacidbindingandotherbiomedicalpropertiesofartificialoligolysines AT roviellov nucleicacidbindingandotherbiomedicalpropertiesofartificialoligolysines |