Increased Expression of Beta-Tubulin in Potato Plants Challenged with Phytophthora infestans
Late blight, caused by Phytophthora infestans is an important foliar disease of potato worldwide. Relatively little is known about the mechanisms of interaction between potato and this fungal pathogen. In the present work, expression of beta-tubulin (TUB) gene was monitored in resistant and suscepti...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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"Vikol publishing" ST Kolesnichenko V.V.
2023-02-01
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| Series: | Journal of Stress Physiology & Biochemistry |
| Subjects: | |
| Online Access: | http://www.jspb.ru/issues/2023/N1/JSPB_2023_1_105-110.pdf |
| _version_ | 1811173135315107840 |
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| author | Antonious Al-Daoude Amina Shoaib Mohammed Jawhar |
| author_facet | Antonious Al-Daoude Amina Shoaib Mohammed Jawhar |
| author_sort | Antonious Al-Daoude |
| collection | DOAJ |
| description | Late blight, caused by Phytophthora infestans is an important foliar disease of potato worldwide. Relatively little is known about the mechanisms of interaction between potato and this fungal pathogen. In the present work, expression of beta-tubulin (TUB) gene was monitored in resistant and susceptible potato cultivars at four early points of infection using quantitative real-time PCR (qPCR). Data showed significant variation in the expression patterns of the TUB gene during potato- P. infestans interactions as compared to the non-inoculated controls. It also shows that β- tubulin gene has a higher expression and faster induction in the resistant cultivar (0.9-fold) as compared with the susceptible one (0.11-fold), 24 hours post inoculation (hpi). However, the maximum expressions were 1.85 –folds in the resistant, and 1.3 –folds in the susceptible one 48 and 72 hpi, respectively. Increased potato TUB expression could be due to plant cytoskeleton rearrangement in response to P. infestans up to 72 hpi, and the subsequent decrease in its expression at 96hpi could be due to plant cell disruption resulting from tissue damage during necrosis. The information obtained from this study highlights crucial remarks into the signaling pathway that accounts for TUB gene expression changes elicited during potato- P. infestans interactions, which can provide testable hypotheses that will need direct future tests to determine how TUB changes may be specified in the defense system. |
| first_indexed | 2024-04-10T17:42:48Z |
| format | Article |
| id | doaj.art-052cadc6aa754137a4bde860b6af0b4c |
| institution | Directory Open Access Journal |
| issn | 1997-0838 |
| language | English |
| last_indexed | 2024-04-10T17:42:48Z |
| publishDate | 2023-02-01 |
| publisher | "Vikol publishing" ST Kolesnichenko V.V. |
| record_format | Article |
| series | Journal of Stress Physiology & Biochemistry |
| spelling | doaj.art-052cadc6aa754137a4bde860b6af0b4c2023-02-03T07:59:10Zeng"Vikol publishing" ST Kolesnichenko V.V.Journal of Stress Physiology & Biochemistry1997-08382023-02-01191105110Increased Expression of Beta-Tubulin in Potato Plants Challenged with Phytophthora infestans Antonious Al-Daoude0Amina Shoaib1Mohammed Jawhar2Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, P.O. Box 6091, Damascus, Syria.Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, P.O. Box 6091, Damascus, Syria.Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, P.O. Box 6091, Damascus, Syria.Late blight, caused by Phytophthora infestans is an important foliar disease of potato worldwide. Relatively little is known about the mechanisms of interaction between potato and this fungal pathogen. In the present work, expression of beta-tubulin (TUB) gene was monitored in resistant and susceptible potato cultivars at four early points of infection using quantitative real-time PCR (qPCR). Data showed significant variation in the expression patterns of the TUB gene during potato- P. infestans interactions as compared to the non-inoculated controls. It also shows that β- tubulin gene has a higher expression and faster induction in the resistant cultivar (0.9-fold) as compared with the susceptible one (0.11-fold), 24 hours post inoculation (hpi). However, the maximum expressions were 1.85 –folds in the resistant, and 1.3 –folds in the susceptible one 48 and 72 hpi, respectively. Increased potato TUB expression could be due to plant cytoskeleton rearrangement in response to P. infestans up to 72 hpi, and the subsequent decrease in its expression at 96hpi could be due to plant cell disruption resulting from tissue damage during necrosis. The information obtained from this study highlights crucial remarks into the signaling pathway that accounts for TUB gene expression changes elicited during potato- P. infestans interactions, which can provide testable hypotheses that will need direct future tests to determine how TUB changes may be specified in the defense system.http://www.jspb.ru/issues/2023/N1/JSPB_2023_1_105-110.pdfsolanum tuberosum l.phytophthora infestansβ- tubulin expressionreal time pcr |
| spellingShingle | Antonious Al-Daoude Amina Shoaib Mohammed Jawhar Increased Expression of Beta-Tubulin in Potato Plants Challenged with Phytophthora infestans Journal of Stress Physiology & Biochemistry solanum tuberosum l. phytophthora infestans β- tubulin expression real time pcr |
| title | Increased Expression of Beta-Tubulin in Potato Plants Challenged with Phytophthora infestans |
| title_full | Increased Expression of Beta-Tubulin in Potato Plants Challenged with Phytophthora infestans |
| title_fullStr | Increased Expression of Beta-Tubulin in Potato Plants Challenged with Phytophthora infestans |
| title_full_unstemmed | Increased Expression of Beta-Tubulin in Potato Plants Challenged with Phytophthora infestans |
| title_short | Increased Expression of Beta-Tubulin in Potato Plants Challenged with Phytophthora infestans |
| title_sort | increased expression of beta tubulin in potato plants challenged with phytophthora infestans |
| topic | solanum tuberosum l. phytophthora infestans β- tubulin expression real time pcr |
| url | http://www.jspb.ru/issues/2023/N1/JSPB_2023_1_105-110.pdf |
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