The effects of locomotion on sensory-evoked haemodynamic responses in the cortex of awake mice
Abstract Investigating neurovascular coupling in awake rodents is becoming ever more popular due, in part, to our increasing knowledge of the profound impacts that anaesthesia can have upon brain physiology. Although awake imaging brings with it many advantages, we still do not fully understand how...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-10195-y |
| _version_ | 1817990432941932544 |
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| author | Beth Eyre Kira Shaw Paul Sharp Luke Boorman Llywelyn Lee Osman Shabir Jason Berwick Clare Howarth |
| author_facet | Beth Eyre Kira Shaw Paul Sharp Luke Boorman Llywelyn Lee Osman Shabir Jason Berwick Clare Howarth |
| author_sort | Beth Eyre |
| collection | DOAJ |
| description | Abstract Investigating neurovascular coupling in awake rodents is becoming ever more popular due, in part, to our increasing knowledge of the profound impacts that anaesthesia can have upon brain physiology. Although awake imaging brings with it many advantages, we still do not fully understand how voluntary locomotion during imaging affects sensory-evoked haemodynamic responses. In this study we investigated how evoked haemodynamic responses can be affected by the amount and timing of locomotion. Using an awake imaging set up, we used 2D-Optical Imaging Spectroscopy (2D-OIS) to measure changes in cerebral haemodynamics within the sensory cortex of the brain during either 2 s whisker stimulation or spontaneous (no whisker stimulation) experiments, whilst animals could walk on a spherical treadmill. We show that locomotion alters haemodynamic responses. The amount and timing of locomotion relative to whisker stimulation is important, and can significantly impact sensory-evoked haemodynamic responses. If locomotion occurred before or during whisker stimulation, the amplitude of the stimulus-evoked haemodynamic response was significantly altered. Therefore, monitoring of locomotion during awake imaging is necessary to ensure that conclusions based on comparisons of evoked haemodynamic responses (e.g., between control and disease groups) are not confounded by the effects of locomotion. |
| first_indexed | 2024-04-14T00:59:59Z |
| format | Article |
| id | doaj.art-052d21db476048348b63a531dd200bc9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-14T00:59:59Z |
| publishDate | 2022-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-052d21db476048348b63a531dd200bc92022-12-22T02:21:27ZengNature PortfolioScientific Reports2045-23222022-04-0112111610.1038/s41598-022-10195-yThe effects of locomotion on sensory-evoked haemodynamic responses in the cortex of awake miceBeth Eyre0Kira Shaw1Paul Sharp2Luke Boorman3Llywelyn Lee4Osman Shabir5Jason Berwick6Clare Howarth7Sheffield Neurovascular Lab, Department of Psychology, University of SheffieldSheffield Neurovascular Lab, Department of Psychology, University of SheffieldSheffield Neurovascular Lab, Department of Psychology, University of SheffieldSheffield Neurovascular Lab, Department of Psychology, University of SheffieldSheffield Neurovascular Lab, Department of Psychology, University of SheffieldSheffield Neurovascular Lab, Department of Psychology, University of SheffieldSheffield Neurovascular Lab, Department of Psychology, University of SheffieldSheffield Neurovascular Lab, Department of Psychology, University of SheffieldAbstract Investigating neurovascular coupling in awake rodents is becoming ever more popular due, in part, to our increasing knowledge of the profound impacts that anaesthesia can have upon brain physiology. Although awake imaging brings with it many advantages, we still do not fully understand how voluntary locomotion during imaging affects sensory-evoked haemodynamic responses. In this study we investigated how evoked haemodynamic responses can be affected by the amount and timing of locomotion. Using an awake imaging set up, we used 2D-Optical Imaging Spectroscopy (2D-OIS) to measure changes in cerebral haemodynamics within the sensory cortex of the brain during either 2 s whisker stimulation or spontaneous (no whisker stimulation) experiments, whilst animals could walk on a spherical treadmill. We show that locomotion alters haemodynamic responses. The amount and timing of locomotion relative to whisker stimulation is important, and can significantly impact sensory-evoked haemodynamic responses. If locomotion occurred before or during whisker stimulation, the amplitude of the stimulus-evoked haemodynamic response was significantly altered. Therefore, monitoring of locomotion during awake imaging is necessary to ensure that conclusions based on comparisons of evoked haemodynamic responses (e.g., between control and disease groups) are not confounded by the effects of locomotion.https://doi.org/10.1038/s41598-022-10195-y |
| spellingShingle | Beth Eyre Kira Shaw Paul Sharp Luke Boorman Llywelyn Lee Osman Shabir Jason Berwick Clare Howarth The effects of locomotion on sensory-evoked haemodynamic responses in the cortex of awake mice Scientific Reports |
| title | The effects of locomotion on sensory-evoked haemodynamic responses in the cortex of awake mice |
| title_full | The effects of locomotion on sensory-evoked haemodynamic responses in the cortex of awake mice |
| title_fullStr | The effects of locomotion on sensory-evoked haemodynamic responses in the cortex of awake mice |
| title_full_unstemmed | The effects of locomotion on sensory-evoked haemodynamic responses in the cortex of awake mice |
| title_short | The effects of locomotion on sensory-evoked haemodynamic responses in the cortex of awake mice |
| title_sort | effects of locomotion on sensory evoked haemodynamic responses in the cortex of awake mice |
| url | https://doi.org/10.1038/s41598-022-10195-y |
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