Comparative Transcriptomic and Metabolomic Analyses of Differences in Trunk Spiral Grain in <i>Pinus yunnanensis</i>
Having a spiral grain is considered to be one of the most important wood properties influencing wood quality. Here, transcriptome profiles and metabolome data were analyzed in the straight grain and twist grain of <i>Pinus yunnanensis</i>. A total of 6644 differential expression genes we...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| author | Peihua Gan Peiling Li Xiaolin Zhang Hailin Li Shaojie Ma Dan Zong Chengzhong He |
| author_facet | Peihua Gan Peiling Li Xiaolin Zhang Hailin Li Shaojie Ma Dan Zong Chengzhong He |
| author_sort | Peihua Gan |
| collection | DOAJ |
| description | Having a spiral grain is considered to be one of the most important wood properties influencing wood quality. Here, transcriptome profiles and metabolome data were analyzed in the straight grain and twist grain of <i>Pinus yunnanensis</i>. A total of 6644 differential expression genes were found between the straight type and the twist type. A total of 126 differentially accumulated metabolites were detected. There were 24 common differential pathways identified from the transcriptome and metabolome, and these pathways were mainly annotated in ABC transporters, arginine and proline metabolism, flavonoid biosynthesis, isoquinoline alkaloid biosynthesis, linoleic acid metabolism, phenylpropanoid, tryptophan metabolism, etc. A weighted gene coexpression network analysis showed that the lightblue4 module was significantly correlated with 2′-deoxyuridine and that transcription factors (basic leucine zipper (bZIP), homeodomain leucine zipper (HD-ZIP), basic helix–loop–helix (bHLH), p-coumarate 3-hydroxylase (C3H), and N-acetylcysteine (NAC)) play important roles in regulating 2′-deoxyuridine, which may be involved in the formation of spiral grains. Meanwhile, the signal transduction of hormones may be related to spiral grain, as previously reported. <i>ARF7</i> and <i>MKK4_5</i>, as indoleacetic acid (IAA)- and ethylene (ET)-related receptors, may explain the contribution of plant hormones in spiral grain. This study provided useful information on spiral grain in <i>P. yunnanensis</i> by transcriptome and metabolome analyses and could lay the foundation for future molecular breeding. |
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| spelling | doaj.art-0530ac4cb64046088c9c0d51dcace1f02023-11-19T14:29:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124191465810.3390/ijms241914658Comparative Transcriptomic and Metabolomic Analyses of Differences in Trunk Spiral Grain in <i>Pinus yunnanensis</i>Peihua Gan0Peiling Li1Xiaolin Zhang2Hailin Li3Shaojie Ma4Dan Zong5Chengzhong He6Key Laboratory for Forest Genetics and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory for Forest Genetics and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory for Forest Genetics and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory for Forest Genetics and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory for Forest Genetics and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory for Forest Genetics and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory for Forest Genetics and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaHaving a spiral grain is considered to be one of the most important wood properties influencing wood quality. Here, transcriptome profiles and metabolome data were analyzed in the straight grain and twist grain of <i>Pinus yunnanensis</i>. A total of 6644 differential expression genes were found between the straight type and the twist type. A total of 126 differentially accumulated metabolites were detected. There were 24 common differential pathways identified from the transcriptome and metabolome, and these pathways were mainly annotated in ABC transporters, arginine and proline metabolism, flavonoid biosynthesis, isoquinoline alkaloid biosynthesis, linoleic acid metabolism, phenylpropanoid, tryptophan metabolism, etc. A weighted gene coexpression network analysis showed that the lightblue4 module was significantly correlated with 2′-deoxyuridine and that transcription factors (basic leucine zipper (bZIP), homeodomain leucine zipper (HD-ZIP), basic helix–loop–helix (bHLH), p-coumarate 3-hydroxylase (C3H), and N-acetylcysteine (NAC)) play important roles in regulating 2′-deoxyuridine, which may be involved in the formation of spiral grains. Meanwhile, the signal transduction of hormones may be related to spiral grain, as previously reported. <i>ARF7</i> and <i>MKK4_5</i>, as indoleacetic acid (IAA)- and ethylene (ET)-related receptors, may explain the contribution of plant hormones in spiral grain. This study provided useful information on spiral grain in <i>P. yunnanensis</i> by transcriptome and metabolome analyses and could lay the foundation for future molecular breeding.https://www.mdpi.com/1422-0067/24/19/14658spiral graintwistdifferential metabolitesgene expressionplant hormone signal transductionterpenoid |
| spellingShingle | Peihua Gan Peiling Li Xiaolin Zhang Hailin Li Shaojie Ma Dan Zong Chengzhong He Comparative Transcriptomic and Metabolomic Analyses of Differences in Trunk Spiral Grain in <i>Pinus yunnanensis</i> International Journal of Molecular Sciences spiral grain twist differential metabolites gene expression plant hormone signal transduction terpenoid |
| title | Comparative Transcriptomic and Metabolomic Analyses of Differences in Trunk Spiral Grain in <i>Pinus yunnanensis</i> |
| title_full | Comparative Transcriptomic and Metabolomic Analyses of Differences in Trunk Spiral Grain in <i>Pinus yunnanensis</i> |
| title_fullStr | Comparative Transcriptomic and Metabolomic Analyses of Differences in Trunk Spiral Grain in <i>Pinus yunnanensis</i> |
| title_full_unstemmed | Comparative Transcriptomic and Metabolomic Analyses of Differences in Trunk Spiral Grain in <i>Pinus yunnanensis</i> |
| title_short | Comparative Transcriptomic and Metabolomic Analyses of Differences in Trunk Spiral Grain in <i>Pinus yunnanensis</i> |
| title_sort | comparative transcriptomic and metabolomic analyses of differences in trunk spiral grain in i pinus yunnanensis i |
| topic | spiral grain twist differential metabolites gene expression plant hormone signal transduction terpenoid |
| url | https://www.mdpi.com/1422-0067/24/19/14658 |
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