| Summary: | Nematodes of the genus <i>Trichinella</i> are among the most widespread parasites of domestic and wild omnivores and predatory animals. The present study aimed to evaluate the antiparasitic effect of <i>Lactobacillus casei</i> ATCC 393 (original) and <i>L. paracasei</i> CNCM in CD-1 mice experimentally infected with <i>Trichinella britovi</i>. Four groups of 20 mice (10 females and 10 males/group) were used, with two control (C) groups and two experimental (E) groups, in which each animal received a daily oral dose of 100 µL of 10<sup>5</sup> CFU/mL probiotics in Ringer’s solution. On day 7, all mice (except the negative control group) were infected orally with <i>Trichinella</i> (100 larvae/animal) as well as the two probiotics. On day 9 post-infection (p.i.), 10 mice/group were euthanized, and the presence of adult parasites in the intestinal content and wall was tested. On day 32 p.i., 10 mice/group were euthanized, then trichinoscopy and artificial digestion were performed to assess the muscle infection with <i>T. britovi</i>. On day 9 p.i., the experimental group pretreated with <i>L. casei</i> ATCC 393 (6.3 ± 3.03) showed a significantly lower number of adult parasites in the intestinal wall compared with the positive control group (24.6 ± 4.78). Additionally, a significantly lower adult parasite count in the intestinal wall was registered in female mice pretreated with <i>L. paracasei</i> CNCM (7.4 ± 4.71) compared to female mice from the positive control (29.0 ± 5.17). No statistically relevant results were obtained concerning the male mice or the data obtained at 32 days p.i., irrespective of mice gender.
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