A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks
The assembly of branched actin networks depends on the heterodimeric capping protein CP/CapZ. Combining cryoEM, in vitro reconstitution and cell biological assays, the authors show that CP not only prevents actin filament elongation but also selectively masks actin filament ends to promote nucleatio...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-09-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-25682-5 |
| _version_ | 1818432587796840448 |
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| author | Johanna Funk Felipe Merino Matthias Schaks Klemens Rottner Stefan Raunser Peter Bieling |
| author_facet | Johanna Funk Felipe Merino Matthias Schaks Klemens Rottner Stefan Raunser Peter Bieling |
| author_sort | Johanna Funk |
| collection | DOAJ |
| description | The assembly of branched actin networks depends on the heterodimeric capping protein CP/CapZ. Combining cryoEM, in vitro reconstitution and cell biological assays, the authors show that CP not only prevents actin filament elongation but also selectively masks actin filament ends to promote nucleation. |
| first_indexed | 2024-12-14T16:07:35Z |
| format | Article |
| id | doaj.art-0538e02cfebe46a795c981704bc20703 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-14T16:07:35Z |
| publishDate | 2021-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-0538e02cfebe46a795c981704bc207032022-12-21T22:55:03ZengNature PortfolioNature Communications2041-17232021-09-0112111710.1038/s41467-021-25682-5A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networksJohanna Funk0Felipe Merino1Matthias Schaks2Klemens Rottner3Stefan Raunser4Peter Bieling5Department of Systemic Cell Biology, Max Planck Institute of Molecular PhysiologyDepartment of Structural Biochemistry, Max Planck Institute of Molecular PhysiologyDivision of Molecular Cell Biology, Zoological Institute, Technische Universität BraunschweigDivision of Molecular Cell Biology, Zoological Institute, Technische Universität BraunschweigDepartment of Structural Biochemistry, Max Planck Institute of Molecular PhysiologyDepartment of Systemic Cell Biology, Max Planck Institute of Molecular PhysiologyThe assembly of branched actin networks depends on the heterodimeric capping protein CP/CapZ. Combining cryoEM, in vitro reconstitution and cell biological assays, the authors show that CP not only prevents actin filament elongation but also selectively masks actin filament ends to promote nucleation.https://doi.org/10.1038/s41467-021-25682-5 |
| spellingShingle | Johanna Funk Felipe Merino Matthias Schaks Klemens Rottner Stefan Raunser Peter Bieling A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks Nature Communications |
| title | A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks |
| title_full | A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks |
| title_fullStr | A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks |
| title_full_unstemmed | A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks |
| title_short | A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks |
| title_sort | barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks |
| url | https://doi.org/10.1038/s41467-021-25682-5 |
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