Ex Vivo Study on the Antioxidant Activity of a Winemaking By-Product Polyphenolic Extract (Taurisolo<sup>®</sup>) on Human Neutrophils
Oxidative stress (OxS) has been linked to several chronic diseases and is recognized to have both major causes and consequences. The use of antioxidant-based nutraceuticals has been licensed as an optimal tool for management of OxS-related diseases. Currently, great interest is focused on the valori...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/7/1009 |
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| author | Giuseppe Annunziata Xavier Capó Maria Magdalena Quetglas-Llabrés Margalida Monserrat-Mesquida Silvia Tejada Josep A. Tur Roberto Ciampaglia Fabrizia Guerra Maria Maisto Gian Carlo Tenore Ettore Novellino Antoni Sureda |
| author_facet | Giuseppe Annunziata Xavier Capó Maria Magdalena Quetglas-Llabrés Margalida Monserrat-Mesquida Silvia Tejada Josep A. Tur Roberto Ciampaglia Fabrizia Guerra Maria Maisto Gian Carlo Tenore Ettore Novellino Antoni Sureda |
| author_sort | Giuseppe Annunziata |
| collection | DOAJ |
| description | Oxidative stress (OxS) has been linked to several chronic diseases and is recognized to have both major causes and consequences. The use of antioxidant-based nutraceuticals has been licensed as an optimal tool for management of OxS-related diseases. Currently, great interest is focused on the valorization of agri-food by-products as a source of bioactive compounds, including polyphenols. In this sense, we evaluated the efficacy of a novel nutraceutical formulation based on polyphenolic extract from <i>Aglianico</i> cultivar grape pomace (registered as Taurisolo<sup>®</sup>). In particular, we tested both native and in vitro gastrointestinal digested forms. The two extracts have been used to treat ex vivo neutrophils from subjects with metabolic syndrome, reporting a marked antioxidant activity of Taurisolo<sup>®</sup>, as shown by its ability to significantly reduce both the levels of reactive oxygen species (ROS) and the activities of catalase and myeloperoxidase in the cell medium after stimulation of neutrophils with phorbol 12-myristate 13-acetate (PMA). Interestingly, we observed an increase in intracellular enzymatic activities in PMA-treated cells, suggesting that Taurisolo<sup>®</sup> polyphenols might be able to activate nuclear factors, up-regulating the expression of this target antioxidant gene. In addition, Taurisolo<sup>®</sup> reversed the increase in malondialdehyde induced by PMA; reduced the expression of pro-inflammatory genes such as cyclooxygenase 2 (COX-2), tumor necrosis factor alpha (TNFα) and myeloperoxidase (MPO); and induced the expression of the anti-inflammatory cytokine IL-10. Overall, these results suggest the efficacy of Taurisolo<sup>®</sup> in contrasting the OxS at blood level, providing evidence for its therapeutic potential in the management of OxS-related pathological conditions in humans. |
| first_indexed | 2024-03-10T10:07:08Z |
| format | Article |
| id | doaj.art-0539154d91d2456f8a4b6cbba3b64aca |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T10:07:08Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-0539154d91d2456f8a4b6cbba3b64aca2023-11-22T01:24:46ZengMDPI AGAntioxidants2076-39212021-06-01107100910.3390/antiox10071009Ex Vivo Study on the Antioxidant Activity of a Winemaking By-Product Polyphenolic Extract (Taurisolo<sup>®</sup>) on Human NeutrophilsGiuseppe Annunziata0Xavier Capó1Maria Magdalena Quetglas-Llabrés2Margalida Monserrat-Mesquida3Silvia Tejada4Josep A. Tur5Roberto Ciampaglia6Fabrizia Guerra7Maria Maisto8Gian Carlo Tenore9Ettore Novellino10Antoni Sureda11NutraPharmaLabs, Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, ItalyResearch Group in Community Nutrition and Oxidative Stress and Health Research Institute of the Balearic Islands (IdISBa), University of Balearic Islands-IUNICS, E-07122 Palma de Mallorca, SpainResearch Group in Community Nutrition and Oxidative Stress and Health Research Institute of the Balearic Islands (IdISBa), University of Balearic Islands-IUNICS, E-07122 Palma de Mallorca, SpainResearch Group in Community Nutrition and Oxidative Stress and Health Research Institute of the Balearic Islands (IdISBa), University of Balearic Islands-IUNICS, E-07122 Palma de Mallorca, SpainResearch Group in Community Nutrition and Oxidative Stress and Health Research Institute of the Balearic Islands (IdISBa), University of Balearic Islands-IUNICS, E-07122 Palma de Mallorca, SpainResearch Group in Community Nutrition and Oxidative Stress and Health Research Institute of the Balearic Islands (IdISBa), University of Balearic Islands-IUNICS, E-07122 Palma de Mallorca, SpainNutraPharmaLabs, Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, ItalyNutraPharmaLabs, Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, ItalyNutraPharmaLabs, Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, ItalyNutraPharmaLabs, Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, ItalyNGN Healthcare—New Generation Nutraceuticals s.r.l., Torrette Via Nazionale 207, 83013 Mercogliano, ItalyResearch Group in Community Nutrition and Oxidative Stress and Health Research Institute of the Balearic Islands (IdISBa), University of Balearic Islands-IUNICS, E-07122 Palma de Mallorca, SpainOxidative stress (OxS) has been linked to several chronic diseases and is recognized to have both major causes and consequences. The use of antioxidant-based nutraceuticals has been licensed as an optimal tool for management of OxS-related diseases. Currently, great interest is focused on the valorization of agri-food by-products as a source of bioactive compounds, including polyphenols. In this sense, we evaluated the efficacy of a novel nutraceutical formulation based on polyphenolic extract from <i>Aglianico</i> cultivar grape pomace (registered as Taurisolo<sup>®</sup>). In particular, we tested both native and in vitro gastrointestinal digested forms. The two extracts have been used to treat ex vivo neutrophils from subjects with metabolic syndrome, reporting a marked antioxidant activity of Taurisolo<sup>®</sup>, as shown by its ability to significantly reduce both the levels of reactive oxygen species (ROS) and the activities of catalase and myeloperoxidase in the cell medium after stimulation of neutrophils with phorbol 12-myristate 13-acetate (PMA). Interestingly, we observed an increase in intracellular enzymatic activities in PMA-treated cells, suggesting that Taurisolo<sup>®</sup> polyphenols might be able to activate nuclear factors, up-regulating the expression of this target antioxidant gene. In addition, Taurisolo<sup>®</sup> reversed the increase in malondialdehyde induced by PMA; reduced the expression of pro-inflammatory genes such as cyclooxygenase 2 (COX-2), tumor necrosis factor alpha (TNFα) and myeloperoxidase (MPO); and induced the expression of the anti-inflammatory cytokine IL-10. Overall, these results suggest the efficacy of Taurisolo<sup>®</sup> in contrasting the OxS at blood level, providing evidence for its therapeutic potential in the management of OxS-related pathological conditions in humans.https://www.mdpi.com/2076-3921/10/7/1009oxidative stressagri-food by-productspolyphenolsnutraceuticalgrapeblood cells |
| spellingShingle | Giuseppe Annunziata Xavier Capó Maria Magdalena Quetglas-Llabrés Margalida Monserrat-Mesquida Silvia Tejada Josep A. Tur Roberto Ciampaglia Fabrizia Guerra Maria Maisto Gian Carlo Tenore Ettore Novellino Antoni Sureda Ex Vivo Study on the Antioxidant Activity of a Winemaking By-Product Polyphenolic Extract (Taurisolo<sup>®</sup>) on Human Neutrophils Antioxidants oxidative stress agri-food by-products polyphenols nutraceutical grape blood cells |
| title | Ex Vivo Study on the Antioxidant Activity of a Winemaking By-Product Polyphenolic Extract (Taurisolo<sup>®</sup>) on Human Neutrophils |
| title_full | Ex Vivo Study on the Antioxidant Activity of a Winemaking By-Product Polyphenolic Extract (Taurisolo<sup>®</sup>) on Human Neutrophils |
| title_fullStr | Ex Vivo Study on the Antioxidant Activity of a Winemaking By-Product Polyphenolic Extract (Taurisolo<sup>®</sup>) on Human Neutrophils |
| title_full_unstemmed | Ex Vivo Study on the Antioxidant Activity of a Winemaking By-Product Polyphenolic Extract (Taurisolo<sup>®</sup>) on Human Neutrophils |
| title_short | Ex Vivo Study on the Antioxidant Activity of a Winemaking By-Product Polyphenolic Extract (Taurisolo<sup>®</sup>) on Human Neutrophils |
| title_sort | ex vivo study on the antioxidant activity of a winemaking by product polyphenolic extract taurisolo sup r sup on human neutrophils |
| topic | oxidative stress agri-food by-products polyphenols nutraceutical grape blood cells |
| url | https://www.mdpi.com/2076-3921/10/7/1009 |
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