| Summary: | Chlorophenol compounds pose a health risk to many organisms due to their toxicity. The present paper presents the estimation of the activation and deactivation energies and the optimum temperatures of 2,4-dichlorophenol degradation by horseradish peroxidase (HRP). The activities of horseradish peroxidase depending on temperature were analyzed. In a mathematical model, describing 2,4-dichlorophenol degradation by HRP was assumed that both the 2,4-dichlorophenol degradation and the deactivation of HRP were first-order reactions by the enzyme concentration. The parameters of the optimum temperatures <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mrow><mi>T</mi></mrow><mrow><mi mathvariant="normal">o</mi><mi mathvariant="normal">p</mi><mi mathvariant="normal">t</mi></mrow></msub></mrow></semantics></math></inline-formula>, the activation energies <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mrow><mi>E</mi></mrow><mrow><mi mathvariant="normal">r</mi></mrow></msub></mrow></semantics></math></inline-formula>, and the deactivation energies <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mrow><mi>E</mi></mrow><mrow><mi mathvariant="normal">d</mi></mrow></msub></mrow></semantics></math></inline-formula> in the process of 2,4-dichlorophenol degradation by HRP immobilized on a modified nanofibrous membrane were determined <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mrow><mi>k</mi></mrow><mrow><mi mathvariant="normal">d</mi></mrow></msub><mo> </mo><mi>and</mi><mo> </mo><msub><mrow><mi>t</mi></mrow><mrow><mn>1</mn><mo>/</mo><mn>2</mn></mrow></msub></mrow></semantics></math></inline-formula> were determined for HRP immobilized at temperatures in the range of 25 °C to 75 °C. Likewise, thermodynamic parameters such as the change in the enthalpy <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msup><mrow><mo>∆</mo><mi>H</mi></mrow><mrow><mo>#</mo></mrow></msup></mrow></semantics></math></inline-formula>, change in entropy <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msup><mrow><mo>∆</mo><mi>S</mi></mrow><mrow><mo>#</mo></mrow></msup></mrow></semantics></math></inline-formula>, the change in Gibbs free energy <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msup><mrow><mo>∆</mo><mi>G</mi></mrow><mrow><mo>#</mo></mrow></msup></mrow></semantics></math></inline-formula> for native HPR and the change in the enthalpy <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mo>∆</mo><mi>H</mi></mrow><mrow><mi>d</mi></mrow><mrow><mo>#</mo></mrow></msubsup></mrow></semantics></math></inline-formula>, change in entropy <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mo>∆</mo><mi>S</mi></mrow><mrow><mi>d</mi></mrow><mrow><mo>#</mo></mrow></msubsup></mrow></semantics></math></inline-formula>, and the change in Gibbs free energy <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mo>∆</mo><mi>G</mi></mrow><mrow><mi>d</mi></mrow><mrow><mo>#</mo></mrow></msubsup></mrow></semantics></math></inline-formula> for deactivated HRP were determined at 25 °C.
|
|---|