Development of a consortium-based microbial agent beneficial to composting of distilled grain waste for Pleurotus ostreatus cultivation
Abstract Background Pleurotus ostreatus is an edible mushroom popularly cultivated worldwide. Distilled grain waste (DGW) is a potential substrate for P. ostreatus cultivation. However, components in DGW restrict P. ostreatus mycelial growth. Therefore, a cost-effective approach to facilitate rapid...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-12-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-021-02089-4 |
| _version_ | 1828338926729822208 |
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| author | Sibao Wu Rongrong Zhou Yuting Ma Yong Fang Guopai Xie Xuezhi Gao Yazhong Xiao Juanjuan Liu Zemin Fang |
| author_facet | Sibao Wu Rongrong Zhou Yuting Ma Yong Fang Guopai Xie Xuezhi Gao Yazhong Xiao Juanjuan Liu Zemin Fang |
| author_sort | Sibao Wu |
| collection | DOAJ |
| description | Abstract Background Pleurotus ostreatus is an edible mushroom popularly cultivated worldwide. Distilled grain waste (DGW) is a potential substrate for P. ostreatus cultivation. However, components in DGW restrict P. ostreatus mycelial growth. Therefore, a cost-effective approach to facilitate rapid P. ostreatus colonization on DGW substrate will benefit P. ostreatus cultivation and DGW recycling. Results Five dominant indigenous bacteria, Sphingobacterium sp. X1, Ureibacillus sp. X2, Pseudoxanthomonas sp. X3, Geobacillus sp. X4, and Aeribacillus sp. X5, were isolated from DGW and selected to develop a consortium-based microbial agent to compost DGW for P. ostreatus cultivation. Microbial agent inoculation led to faster carbohydrate metabolism, a higher temperature (73.2 vs. 71.2 °C), a longer thermophilic phase (5 vs. 3 days), and significant dynamic changes in microbial community composition and diversity in composts than those of the controls. Metagenomic analysis showed the enhanced microbial metabolisms, such as xenobiotic biodegradation and metabolism and terpenoid and polyketide metabolism, during the mesophilic phase after microbial agent inoculation, which may facilitate the fungal colonization on the substrate. In accordance with the bioinformatic analysis, a faster colonization of P. ostreatus was observed in the composts with microbial inoculation than in control after composting for 48 h, as indicated from substantially higher fungal ergosterol content, faster lignocellulose degradation, and higher lignocellulase activities in the former than in the latter. The final mushroom yield shared no significant difference between composts with microbial inoculation and control, with 0.67 ± 0.05 and 0.60 ± 0.04 kg fresh mushroom/kg DGW, respectively (p > 0.05). Conclusion The consortium-based microbial agent comprised indigenous microorganisms showing application potential in composting DGW for providing substrate for P. ostreatus cultivation and will provide an alternative to facilitate DGW recycling. |
| first_indexed | 2024-04-13T22:35:53Z |
| format | Article |
| id | doaj.art-053b5a8188db406ea0ecce46b272d9b5 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T22:35:53Z |
| publishDate | 2021-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-053b5a8188db406ea0ecce46b272d9b52022-12-22T02:26:47ZengBMCBiotechnology for Biofuels1754-68342021-12-0114111610.1186/s13068-021-02089-4Development of a consortium-based microbial agent beneficial to composting of distilled grain waste for Pleurotus ostreatus cultivationSibao Wu0Rongrong Zhou1Yuting Ma2Yong Fang3Guopai Xie4Xuezhi Gao5Yazhong Xiao6Juanjuan Liu7Zemin Fang8School of Life Sciences, Anhui UniversitySchool of Life Sciences, Anhui UniversitySchool of Life Sciences, Anhui UniversitySchool of Life Sciences, Anhui UniversityAnhui Golden Seed Winery Co., LTDLivestock and Poultry Breeding Service Center of Fuyang CitySchool of Life Sciences, Anhui UniversitySchool of Life Sciences, Anhui UniversitySchool of Life Sciences, Anhui UniversityAbstract Background Pleurotus ostreatus is an edible mushroom popularly cultivated worldwide. Distilled grain waste (DGW) is a potential substrate for P. ostreatus cultivation. However, components in DGW restrict P. ostreatus mycelial growth. Therefore, a cost-effective approach to facilitate rapid P. ostreatus colonization on DGW substrate will benefit P. ostreatus cultivation and DGW recycling. Results Five dominant indigenous bacteria, Sphingobacterium sp. X1, Ureibacillus sp. X2, Pseudoxanthomonas sp. X3, Geobacillus sp. X4, and Aeribacillus sp. X5, were isolated from DGW and selected to develop a consortium-based microbial agent to compost DGW for P. ostreatus cultivation. Microbial agent inoculation led to faster carbohydrate metabolism, a higher temperature (73.2 vs. 71.2 °C), a longer thermophilic phase (5 vs. 3 days), and significant dynamic changes in microbial community composition and diversity in composts than those of the controls. Metagenomic analysis showed the enhanced microbial metabolisms, such as xenobiotic biodegradation and metabolism and terpenoid and polyketide metabolism, during the mesophilic phase after microbial agent inoculation, which may facilitate the fungal colonization on the substrate. In accordance with the bioinformatic analysis, a faster colonization of P. ostreatus was observed in the composts with microbial inoculation than in control after composting for 48 h, as indicated from substantially higher fungal ergosterol content, faster lignocellulose degradation, and higher lignocellulase activities in the former than in the latter. The final mushroom yield shared no significant difference between composts with microbial inoculation and control, with 0.67 ± 0.05 and 0.60 ± 0.04 kg fresh mushroom/kg DGW, respectively (p > 0.05). Conclusion The consortium-based microbial agent comprised indigenous microorganisms showing application potential in composting DGW for providing substrate for P. ostreatus cultivation and will provide an alternative to facilitate DGW recycling.https://doi.org/10.1186/s13068-021-02089-4Pleurotus ostreatus cultivationMicrobial agentDistilled grain wasteCompostingMicrobial metabolism |
| spellingShingle | Sibao Wu Rongrong Zhou Yuting Ma Yong Fang Guopai Xie Xuezhi Gao Yazhong Xiao Juanjuan Liu Zemin Fang Development of a consortium-based microbial agent beneficial to composting of distilled grain waste for Pleurotus ostreatus cultivation Biotechnology for Biofuels Pleurotus ostreatus cultivation Microbial agent Distilled grain waste Composting Microbial metabolism |
| title | Development of a consortium-based microbial agent beneficial to composting of distilled grain waste for Pleurotus ostreatus cultivation |
| title_full | Development of a consortium-based microbial agent beneficial to composting of distilled grain waste for Pleurotus ostreatus cultivation |
| title_fullStr | Development of a consortium-based microbial agent beneficial to composting of distilled grain waste for Pleurotus ostreatus cultivation |
| title_full_unstemmed | Development of a consortium-based microbial agent beneficial to composting of distilled grain waste for Pleurotus ostreatus cultivation |
| title_short | Development of a consortium-based microbial agent beneficial to composting of distilled grain waste for Pleurotus ostreatus cultivation |
| title_sort | development of a consortium based microbial agent beneficial to composting of distilled grain waste for pleurotus ostreatus cultivation |
| topic | Pleurotus ostreatus cultivation Microbial agent Distilled grain waste Composting Microbial metabolism |
| url | https://doi.org/10.1186/s13068-021-02089-4 |
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