Computational identification of significant immunogenic epitopes of the putative outer membrane proteins from Mycobacterium tuberculosis
Abstract Novel vaccines are required to effectively combat the epidemic spread of tuberculosis. Using in silico approaches, this study focuses on prediction of potential B cell and T cell binding immunogenic epitopes for 30 putative outer membrane proteins of Mtb. Among these, certain immunodominant...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-021-00148-9 |
| _version_ | 1797204368827613184 |
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| author | Shobana Sundar Lokesh Thangamani Shanmughavel Piramanayagam |
| author_facet | Shobana Sundar Lokesh Thangamani Shanmughavel Piramanayagam |
| author_sort | Shobana Sundar |
| collection | DOAJ |
| description | Abstract Novel vaccines are required to effectively combat the epidemic spread of tuberculosis. Using in silico approaches, this study focuses on prediction of potential B cell and T cell binding immunogenic epitopes for 30 putative outer membrane proteins of Mtb. Among these, certain immunodominant epitopes of Rv0172, Rv0295c, Rv1006, Rv2264c, and Rv2525c were found, which are capable of binding B-cell and a maximum number of MHC alleles. The selected immunodominant epitopes were screened for their allergenic and antigenic properties, their percentage identity against the human proteome and their structural properties. Further, the binding efficacy of the immunodominant epitopes of Rv0295c and Rv1006 with HLA-DRB1*04:01 was analyzed using molecular docking and molecular dynamics studies. Hence, the in silico-derived immunogenic peptides (epitopes) could potentially be used for the design of subunit vaccines against tuberculosis. |
| first_indexed | 2024-04-24T08:34:07Z |
| format | Article |
| id | doaj.art-054171b7fd4f49078c721cebfd34d995 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:34:07Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-054171b7fd4f49078c721cebfd34d9952024-04-16T18:06:31ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202021-03-0119111010.1186/s43141-021-00148-9Computational identification of significant immunogenic epitopes of the putative outer membrane proteins from Mycobacterium tuberculosisShobana Sundar0Lokesh Thangamani1Shanmughavel Piramanayagam2Computational Biology Lab, Department of Bioinformatics, Bharathiar UniversityComputational Biology Lab, Department of Bioinformatics, Bharathiar UniversityComputational Biology Lab, Department of Bioinformatics, Bharathiar UniversityAbstract Novel vaccines are required to effectively combat the epidemic spread of tuberculosis. Using in silico approaches, this study focuses on prediction of potential B cell and T cell binding immunogenic epitopes for 30 putative outer membrane proteins of Mtb. Among these, certain immunodominant epitopes of Rv0172, Rv0295c, Rv1006, Rv2264c, and Rv2525c were found, which are capable of binding B-cell and a maximum number of MHC alleles. The selected immunodominant epitopes were screened for their allergenic and antigenic properties, their percentage identity against the human proteome and their structural properties. Further, the binding efficacy of the immunodominant epitopes of Rv0295c and Rv1006 with HLA-DRB1*04:01 was analyzed using molecular docking and molecular dynamics studies. Hence, the in silico-derived immunogenic peptides (epitopes) could potentially be used for the design of subunit vaccines against tuberculosis.https://doi.org/10.1186/s43141-021-00148-9ImmunoinformaticsOuter membrane proteinsMycobacterium tuberculosisEpitopes |
| spellingShingle | Shobana Sundar Lokesh Thangamani Shanmughavel Piramanayagam Computational identification of significant immunogenic epitopes of the putative outer membrane proteins from Mycobacterium tuberculosis Journal of Genetic Engineering and Biotechnology Immunoinformatics Outer membrane proteins Mycobacterium tuberculosis Epitopes |
| title | Computational identification of significant immunogenic epitopes of the putative outer membrane proteins from Mycobacterium tuberculosis |
| title_full | Computational identification of significant immunogenic epitopes of the putative outer membrane proteins from Mycobacterium tuberculosis |
| title_fullStr | Computational identification of significant immunogenic epitopes of the putative outer membrane proteins from Mycobacterium tuberculosis |
| title_full_unstemmed | Computational identification of significant immunogenic epitopes of the putative outer membrane proteins from Mycobacterium tuberculosis |
| title_short | Computational identification of significant immunogenic epitopes of the putative outer membrane proteins from Mycobacterium tuberculosis |
| title_sort | computational identification of significant immunogenic epitopes of the putative outer membrane proteins from mycobacterium tuberculosis |
| topic | Immunoinformatics Outer membrane proteins Mycobacterium tuberculosis Epitopes |
| url | https://doi.org/10.1186/s43141-021-00148-9 |
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