Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate
Hyperuricemia, an abnormally high level of blood uric acid, is a major risk factor for gout. Although xanthine oxidase inhibitors were clinically used to lower blood uric acid level, the concerned side effects restricted their utilization. In this study, strictinin, an abundant polyphenol in Pu’er t...
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2023-02-01
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| Online Access: | https://www.mdpi.com/2079-7737/12/2/329 |
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| author | Kuo-Ching Huang Yu-Ting Chang Rosita Pranata Yung-Hsuan Cheng Yu-Chi Chen Ping-Chung Kuo Yi-Hsuan Huang Jason T. C. Tzen Rong-Jane Chen |
| author_facet | Kuo-Ching Huang Yu-Ting Chang Rosita Pranata Yung-Hsuan Cheng Yu-Chi Chen Ping-Chung Kuo Yi-Hsuan Huang Jason T. C. Tzen Rong-Jane Chen |
| author_sort | Kuo-Ching Huang |
| collection | DOAJ |
| description | Hyperuricemia, an abnormally high level of blood uric acid, is a major risk factor for gout. Although xanthine oxidase inhibitors were clinically used to lower blood uric acid level, the concerned side effects restricted their utilization. In this study, strictinin, an abundant polyphenol in Pu’er tea, was evaluated for its preventive effects on hyperuricemia. The results showed that the xanthine oxidase activity, uric acid production, and inflammation in AML12 mouse hepatocytes treated with xanthine were significantly reduced by the supplementation of strictinin. Detailed analyses revealed that strictinin inhibited xanthine-induced NLRP3 inflammasome activation. Consistently, the elevated blood uric acid level and the enhanced xanthine oxidase activity in mice treated with potassium oxonate were effectively diminished by strictinin supplementation. Moreover, for the first time, strictinin was found to promote healthy gut microbiota. Overall, strictinin possesses a great potential to be utilized as a functional ingredient for the prevention of hyperuricemia. |
| first_indexed | 2024-03-11T09:07:38Z |
| format | Article |
| id | doaj.art-0543026ddba84210ac46bcddfd81a3a8 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T09:07:38Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-0543026ddba84210ac46bcddfd81a3a82023-11-16T19:14:54ZengMDPI AGBiology2079-77372023-02-0112232910.3390/biology12020329Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium OxonateKuo-Ching Huang0Yu-Ting Chang1Rosita Pranata2Yung-Hsuan Cheng3Yu-Chi Chen4Ping-Chung Kuo5Yi-Hsuan Huang6Jason T. C. Tzen7Rong-Jane Chen8Division of Nephrology, Department of Internal Medicine, Chi Mei Hospital, Tainan 736, TaiwanDepartment of Food Safety/Hygiene and Risk Management, College of Medicine, National Cheng Kung University, Tainan 701, TaiwanDepartment of Food Safety/Hygiene and Risk Management, College of Medicine, National Cheng Kung University, Tainan 701, TaiwanDepartment of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan 701, TaiwanDepartment of Biotechnology, National Kaohsiung Normal University, Kaohsiung 824, TaiwanSchool of Pharmacy, College of Medicine, National Cheng Kung University, Tainan 701, TaiwanDepartment of Food Safety/Hygiene and Risk Management, College of Medicine, National Cheng Kung University, Tainan 701, TaiwanGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, TaiwanDepartment of Food Safety/Hygiene and Risk Management, College of Medicine, National Cheng Kung University, Tainan 701, TaiwanHyperuricemia, an abnormally high level of blood uric acid, is a major risk factor for gout. Although xanthine oxidase inhibitors were clinically used to lower blood uric acid level, the concerned side effects restricted their utilization. In this study, strictinin, an abundant polyphenol in Pu’er tea, was evaluated for its preventive effects on hyperuricemia. The results showed that the xanthine oxidase activity, uric acid production, and inflammation in AML12 mouse hepatocytes treated with xanthine were significantly reduced by the supplementation of strictinin. Detailed analyses revealed that strictinin inhibited xanthine-induced NLRP3 inflammasome activation. Consistently, the elevated blood uric acid level and the enhanced xanthine oxidase activity in mice treated with potassium oxonate were effectively diminished by strictinin supplementation. Moreover, for the first time, strictinin was found to promote healthy gut microbiota. Overall, strictinin possesses a great potential to be utilized as a functional ingredient for the prevention of hyperuricemia.https://www.mdpi.com/2079-7737/12/2/329hyperuricemiaNLRP3 inflammasomestrictininuric acidxanthine |
| spellingShingle | Kuo-Ching Huang Yu-Ting Chang Rosita Pranata Yung-Hsuan Cheng Yu-Chi Chen Ping-Chung Kuo Yi-Hsuan Huang Jason T. C. Tzen Rong-Jane Chen Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate Biology hyperuricemia NLRP3 inflammasome strictinin uric acid xanthine |
| title | Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate |
| title_full | Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate |
| title_fullStr | Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate |
| title_full_unstemmed | Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate |
| title_short | Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate |
| title_sort | alleviation of hyperuricemia by strictinin in aml12 mouse hepatocytes treated with xanthine and in mice treated with potassium oxonate |
| topic | hyperuricemia NLRP3 inflammasome strictinin uric acid xanthine |
| url | https://www.mdpi.com/2079-7737/12/2/329 |
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