Impacts of Amla (Phyllanthus emblica) fruit extract on growth, skin mucosal and serum immunities, and disease resistance of Nile tilapia (Oreochromis niloticus) raised under biofloc system
This study aimed to investigate the effects of Amla (Phyllanthus emblica) fruit extract (AFE) on the growth, skin mucosal and serum immunity, and disease resistance of Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae. Three hundred fish (10.48 ± 0.56 g fish-1) were distributed i...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-02-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513421003707 |
| _version_ | 1798014737717395456 |
|---|---|
| author | Hien Van Doan Chompunut Lumsangkul Korawan Sringarm Seyed Hossein Hoseinifar Mahmoud A.O. Dawood Ehab El-Haroun Ramasamy Harikrishnan Sanchai Jaturasitha Marina Paolucci |
| author_facet | Hien Van Doan Chompunut Lumsangkul Korawan Sringarm Seyed Hossein Hoseinifar Mahmoud A.O. Dawood Ehab El-Haroun Ramasamy Harikrishnan Sanchai Jaturasitha Marina Paolucci |
| author_sort | Hien Van Doan |
| collection | DOAJ |
| description | This study aimed to investigate the effects of Amla (Phyllanthus emblica) fruit extract (AFE) on the growth, skin mucosal and serum immunity, and disease resistance of Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae. Three hundred fish (10.48 ± 0.56 g fish-1) were distributed in 15 tanks with 20 fish per tank and fed basal diet supplemented with 0, 5, 10, 20, and 40 mg kg-1 AFE for eight weeks. The completed randomized design with three replications was applied. Growth performance, peroxidase, alternative complement, phagocytosis, and respiratory burst activities were determined at weeks 4 and 8. The challenge study was conducted at the end of the experiment. Based on the results, Nile tilapia fed the AFE diets significantly enhanced skin mucus lysozyme and peroxidase activities (P ≤ 0.05) post-feeding. The highest levels were observed in the group that received 20 mg kg-1 of AFE (P ≤ 0.05), whereas the control group presented the lowest levels of lysosome and peroxidase activities. Similarly, incorporation of 20 mg kg-1 of AFE brought about the maximum levels of (P ≤ 0.05) serum lysozyme, peroxidase, alternative complement, phagocytosis, and respiratory burst activities in contrast to the other treatments; whereas fish fed the control diet generated the lowest immune parameter values (P ≤ 0.05). In the challenge study, the Nile tilapia’s relative percent survival (RPS) was 52.38%, 61.90%, 80.95%, and 47.62% in Diet 2, Diet 3, Diet 4, and Diet 5, respectively. Fish fed 20 mg kg-1 AFE showed significantly (P < 0.05) higher RPS, as well as the highest resistance towards S. agalactiae. The inclusion of AFE for 8 weeks resulted in a significant (P < 0.05) enhancement of growth parameters, with the highest growth rate observed in the 20 mg kg-1 AFE group. In conclusion, dietary supplementation with 20 mg AFE kg-1 may be used as a growth promoter and immunostimulant in Nile tilapia aquaculture. |
| first_indexed | 2024-04-11T15:22:04Z |
| format | Article |
| id | doaj.art-05433123687640a887a29b0b5c7fdf59 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-11T15:22:04Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-05433123687640a887a29b0b5c7fdf592022-12-22T04:16:20ZengElsevierAquaculture Reports2352-51342022-02-0122100953Impacts of Amla (Phyllanthus emblica) fruit extract on growth, skin mucosal and serum immunities, and disease resistance of Nile tilapia (Oreochromis niloticus) raised under biofloc systemHien Van Doan0Chompunut Lumsangkul1Korawan Sringarm2Seyed Hossein Hoseinifar3Mahmoud A.O. Dawood4Ehab El-Haroun5Ramasamy Harikrishnan6Sanchai Jaturasitha7Marina Paolucci8Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand; Corresponding authors.Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, IranDepartment of Animal Production, Faculty of Agriculture, Kafrelsheikh University, EgyptFish Nutrition research laboratory, Cairo University, EgyptDepartment of Zoology, Pachaiyappa's College for Men, Kanchipuram 631 501, Tamil Nadu, IndiaScience and Technology Research Institute, Chiang Mai University, 239 Huay Keaw Rd., Suthep, Muang, Chiang Mai 50200, Thailand; Corresponding authors.Department of Science and Technologies, Via De Sanctis,snc, Benevento, ItalyThis study aimed to investigate the effects of Amla (Phyllanthus emblica) fruit extract (AFE) on the growth, skin mucosal and serum immunity, and disease resistance of Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae. Three hundred fish (10.48 ± 0.56 g fish-1) were distributed in 15 tanks with 20 fish per tank and fed basal diet supplemented with 0, 5, 10, 20, and 40 mg kg-1 AFE for eight weeks. The completed randomized design with three replications was applied. Growth performance, peroxidase, alternative complement, phagocytosis, and respiratory burst activities were determined at weeks 4 and 8. The challenge study was conducted at the end of the experiment. Based on the results, Nile tilapia fed the AFE diets significantly enhanced skin mucus lysozyme and peroxidase activities (P ≤ 0.05) post-feeding. The highest levels were observed in the group that received 20 mg kg-1 of AFE (P ≤ 0.05), whereas the control group presented the lowest levels of lysosome and peroxidase activities. Similarly, incorporation of 20 mg kg-1 of AFE brought about the maximum levels of (P ≤ 0.05) serum lysozyme, peroxidase, alternative complement, phagocytosis, and respiratory burst activities in contrast to the other treatments; whereas fish fed the control diet generated the lowest immune parameter values (P ≤ 0.05). In the challenge study, the Nile tilapia’s relative percent survival (RPS) was 52.38%, 61.90%, 80.95%, and 47.62% in Diet 2, Diet 3, Diet 4, and Diet 5, respectively. Fish fed 20 mg kg-1 AFE showed significantly (P < 0.05) higher RPS, as well as the highest resistance towards S. agalactiae. The inclusion of AFE for 8 weeks resulted in a significant (P < 0.05) enhancement of growth parameters, with the highest growth rate observed in the 20 mg kg-1 AFE group. In conclusion, dietary supplementation with 20 mg AFE kg-1 may be used as a growth promoter and immunostimulant in Nile tilapia aquaculture.http://www.sciencedirect.com/science/article/pii/S2352513421003707Plant extractNile tilapiaGrowth performanceImmune responseStreptococcus agalactiae |
| spellingShingle | Hien Van Doan Chompunut Lumsangkul Korawan Sringarm Seyed Hossein Hoseinifar Mahmoud A.O. Dawood Ehab El-Haroun Ramasamy Harikrishnan Sanchai Jaturasitha Marina Paolucci Impacts of Amla (Phyllanthus emblica) fruit extract on growth, skin mucosal and serum immunities, and disease resistance of Nile tilapia (Oreochromis niloticus) raised under biofloc system Aquaculture Reports Plant extract Nile tilapia Growth performance Immune response Streptococcus agalactiae |
| title | Impacts of Amla (Phyllanthus emblica) fruit extract on growth, skin mucosal and serum immunities, and disease resistance of Nile tilapia (Oreochromis niloticus) raised under biofloc system |
| title_full | Impacts of Amla (Phyllanthus emblica) fruit extract on growth, skin mucosal and serum immunities, and disease resistance of Nile tilapia (Oreochromis niloticus) raised under biofloc system |
| title_fullStr | Impacts of Amla (Phyllanthus emblica) fruit extract on growth, skin mucosal and serum immunities, and disease resistance of Nile tilapia (Oreochromis niloticus) raised under biofloc system |
| title_full_unstemmed | Impacts of Amla (Phyllanthus emblica) fruit extract on growth, skin mucosal and serum immunities, and disease resistance of Nile tilapia (Oreochromis niloticus) raised under biofloc system |
| title_short | Impacts of Amla (Phyllanthus emblica) fruit extract on growth, skin mucosal and serum immunities, and disease resistance of Nile tilapia (Oreochromis niloticus) raised under biofloc system |
| title_sort | impacts of amla phyllanthus emblica fruit extract on growth skin mucosal and serum immunities and disease resistance of nile tilapia oreochromis niloticus raised under biofloc system |
| topic | Plant extract Nile tilapia Growth performance Immune response Streptococcus agalactiae |
| url | http://www.sciencedirect.com/science/article/pii/S2352513421003707 |
| work_keys_str_mv | AT hienvandoan impactsofamlaphyllanthusemblicafruitextractongrowthskinmucosalandserumimmunitiesanddiseaseresistanceofniletilapiaoreochromisniloticusraisedunderbioflocsystem AT chompunutlumsangkul impactsofamlaphyllanthusemblicafruitextractongrowthskinmucosalandserumimmunitiesanddiseaseresistanceofniletilapiaoreochromisniloticusraisedunderbioflocsystem AT korawansringarm impactsofamlaphyllanthusemblicafruitextractongrowthskinmucosalandserumimmunitiesanddiseaseresistanceofniletilapiaoreochromisniloticusraisedunderbioflocsystem AT seyedhosseinhoseinifar impactsofamlaphyllanthusemblicafruitextractongrowthskinmucosalandserumimmunitiesanddiseaseresistanceofniletilapiaoreochromisniloticusraisedunderbioflocsystem AT mahmoudaodawood impactsofamlaphyllanthusemblicafruitextractongrowthskinmucosalandserumimmunitiesanddiseaseresistanceofniletilapiaoreochromisniloticusraisedunderbioflocsystem AT ehabelharoun impactsofamlaphyllanthusemblicafruitextractongrowthskinmucosalandserumimmunitiesanddiseaseresistanceofniletilapiaoreochromisniloticusraisedunderbioflocsystem AT ramasamyharikrishnan impactsofamlaphyllanthusemblicafruitextractongrowthskinmucosalandserumimmunitiesanddiseaseresistanceofniletilapiaoreochromisniloticusraisedunderbioflocsystem AT sanchaijaturasitha impactsofamlaphyllanthusemblicafruitextractongrowthskinmucosalandserumimmunitiesanddiseaseresistanceofniletilapiaoreochromisniloticusraisedunderbioflocsystem AT marinapaolucci impactsofamlaphyllanthusemblicafruitextractongrowthskinmucosalandserumimmunitiesanddiseaseresistanceofniletilapiaoreochromisniloticusraisedunderbioflocsystem |