An Experimental Study on Performance of Bacillus pumilus KC845305 and Bacillus flexus KC845306 in Bacterial Concrete
In recent years, concrete has become an important versatile construction material. This paper evaluates the strength obtained by concrete with the influence of bacteria. The bacterial strains were isolated from calcareous sludge and urea ware house. The bacterial strains were identified through 16sr...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tamkang University Press
2023-04-01
|
| Series: | Journal of Applied Science and Engineering |
| Subjects: | |
| Online Access: | http://jase.tku.edu.tw/articles/jase-202003-23-1-0001 |
| _version_ | 1827958130406850560 |
|---|---|
| author | Anand Elumalai G. Mohan Ganesh Kalaichelvan Gurumurthy |
| author_facet | Anand Elumalai G. Mohan Ganesh Kalaichelvan Gurumurthy |
| author_sort | Anand Elumalai |
| collection | DOAJ |
| description | In recent years, concrete has become an important versatile construction material. This paper evaluates the strength obtained by concrete with the influence of bacteria. The bacterial strains were isolated from calcareous sludge and urea ware house. The bacterial strains were identified through 16srRNA gene sequencing as Bacillus pumilus and Bacillus flexus. Using these strains, an experiment on cylinder and prisms cast was performed. Compressive strength, split tensile and flexural tests were conducted at the age of 7, 28 and 56 days with ultrasonic pulse velocity and rebound hammer. The results were compared with Bacillus cohnii MTCC 3616 obtained from microbial type culture collection and gene bank, Chandigarh, India. Based on the experimental results, the improvement in the mechanical strength is due to the deposition of calcite crystals on the bacterial cell surfaces within the pores which enhanced the strength of concrete and reduced porosity and permeability. |
| first_indexed | 2024-04-09T15:30:44Z |
| format | Article |
| id | doaj.art-0543feb9dba747d386d6476b79897f7c |
| institution | Directory Open Access Journal |
| issn | 2708-9967 2708-9975 |
| language | English |
| last_indexed | 2024-04-09T15:30:44Z |
| publishDate | 2023-04-01 |
| publisher | Tamkang University Press |
| record_format | Article |
| series | Journal of Applied Science and Engineering |
| spelling | doaj.art-0543feb9dba747d386d6476b79897f7c2023-04-28T06:55:50ZengTamkang University PressJournal of Applied Science and Engineering2708-99672708-99752023-04-01231 18 10.6180/jase.202003_23(1).0001An Experimental Study on Performance of Bacillus pumilus KC845305 and Bacillus flexus KC845306 in Bacterial ConcreteAnand Elumalai0G. Mohan Ganesh1Kalaichelvan Gurumurthy2School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore-632 014, TN, IndiaDepartment of Structural and Geotechnical Engineering, Vellore Institute of Technology, Vellore-632014, TN, IndiaVIT School of Agricultural Innovations and Advance Learning (VAIAL), Vellore Institute of Technology, Vellore-632014. TN, India.In recent years, concrete has become an important versatile construction material. This paper evaluates the strength obtained by concrete with the influence of bacteria. The bacterial strains were isolated from calcareous sludge and urea ware house. The bacterial strains were identified through 16srRNA gene sequencing as Bacillus pumilus and Bacillus flexus. Using these strains, an experiment on cylinder and prisms cast was performed. Compressive strength, split tensile and flexural tests were conducted at the age of 7, 28 and 56 days with ultrasonic pulse velocity and rebound hammer. The results were compared with Bacillus cohnii MTCC 3616 obtained from microbial type culture collection and gene bank, Chandigarh, India. Based on the experimental results, the improvement in the mechanical strength is due to the deposition of calcite crystals on the bacterial cell surfaces within the pores which enhanced the strength of concrete and reduced porosity and permeability.http://jase.tku.edu.tw/articles/jase-202003-23-1-0001biomineralizationbacterial concreteultra sonic pulse velocityscanning electron microscope (sem)x-ray diffraction (xrd) |
| spellingShingle | Anand Elumalai G. Mohan Ganesh Kalaichelvan Gurumurthy An Experimental Study on Performance of Bacillus pumilus KC845305 and Bacillus flexus KC845306 in Bacterial Concrete Journal of Applied Science and Engineering biomineralization bacterial concrete ultra sonic pulse velocity scanning electron microscope (sem) x-ray diffraction (xrd) |
| title | An Experimental Study on Performance of Bacillus pumilus KC845305 and Bacillus flexus KC845306 in Bacterial Concrete |
| title_full | An Experimental Study on Performance of Bacillus pumilus KC845305 and Bacillus flexus KC845306 in Bacterial Concrete |
| title_fullStr | An Experimental Study on Performance of Bacillus pumilus KC845305 and Bacillus flexus KC845306 in Bacterial Concrete |
| title_full_unstemmed | An Experimental Study on Performance of Bacillus pumilus KC845305 and Bacillus flexus KC845306 in Bacterial Concrete |
| title_short | An Experimental Study on Performance of Bacillus pumilus KC845305 and Bacillus flexus KC845306 in Bacterial Concrete |
| title_sort | experimental study on performance of bacillus pumilus kc845305 and bacillus flexus kc845306 in bacterial concrete |
| topic | biomineralization bacterial concrete ultra sonic pulse velocity scanning electron microscope (sem) x-ray diffraction (xrd) |
| url | http://jase.tku.edu.tw/articles/jase-202003-23-1-0001 |
| work_keys_str_mv | AT anandelumalai anexperimentalstudyonperformanceofbacilluspumiluskc845305andbacillusflexuskc845306inbacterialconcrete AT gmohanganesh anexperimentalstudyonperformanceofbacilluspumiluskc845305andbacillusflexuskc845306inbacterialconcrete AT kalaichelvangurumurthy anexperimentalstudyonperformanceofbacilluspumiluskc845305andbacillusflexuskc845306inbacterialconcrete AT anandelumalai experimentalstudyonperformanceofbacilluspumiluskc845305andbacillusflexuskc845306inbacterialconcrete AT gmohanganesh experimentalstudyonperformanceofbacilluspumiluskc845305andbacillusflexuskc845306inbacterialconcrete AT kalaichelvangurumurthy experimentalstudyonperformanceofbacilluspumiluskc845305andbacillusflexuskc845306inbacterialconcrete |