In vitro assessment of the effect of four polysaccharides on intestinal bacteria of mice with colitis
Abstract Polysaccharides are typically resistant to digestion in the gastrointestinal tract and are instead degraded by gut microbiota in the colon. As such, they are commonly employed as carriers for colon‐targeted drugs, with the potential to regulate gut microbiota. Pectin, carrageenan, guar gum,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-09-01
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| Series: | Food Frontiers |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fft2.270 |
| _version_ | 1797671306616897536 |
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| author | Shanshan Zhang Yonggan Sun Qixing Nie Jielun Hu Wenwen Su Zheyu Guo Yanli Zhang Shaoping Nie |
| author_facet | Shanshan Zhang Yonggan Sun Qixing Nie Jielun Hu Wenwen Su Zheyu Guo Yanli Zhang Shaoping Nie |
| author_sort | Shanshan Zhang |
| collection | DOAJ |
| description | Abstract Polysaccharides are typically resistant to digestion in the gastrointestinal tract and are instead degraded by gut microbiota in the colon. As such, they are commonly employed as carriers for colon‐targeted drugs, with the potential to regulate gut microbiota. Pectin, carrageenan, guar gum, and xanthan gum are commonly used polysaccharide carriers, but their degradation in the gut microbiota under colitis conditions as well as their effects on gut microbiota remains unclear. In this study, we performed the in vitro fermentation of these four polysaccharides using colonic content microbiota from mice with colitis and evaluated the degree of polysaccharide degradation and the effects on pH, short‐chain fatty acids, and gut microbiota. Our findings indicate that pectin and guar gum had a greater degree of degradation and promoted the production of butyrate, inhibited the proliferation of harmful bacteria (e.g., Enterobacteriaceae), and increased beneficial bacteria (e.g., Bifidobacterium). In contrast, carrageenan and xanthan gum promote the proliferation of Enterobacteriaceae. These results provide theoretical guidance for the selection of drug delivery carriers for inflammatory bowel disease treatment and provide information on the relationship between polysaccharides and gut microbiota. |
| first_indexed | 2024-03-11T21:13:25Z |
| format | Article |
| id | doaj.art-0549630daa374682af99b1fde8010153 |
| institution | Directory Open Access Journal |
| issn | 2643-8429 |
| language | English |
| last_indexed | 2024-03-11T21:13:25Z |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Frontiers |
| spelling | doaj.art-0549630daa374682af99b1fde80101532023-09-29T05:33:43ZengWileyFood Frontiers2643-84292023-09-01431462147110.1002/fft2.270In vitro assessment of the effect of four polysaccharides on intestinal bacteria of mice with colitisShanshan Zhang0Yonggan Sun1Qixing Nie2Jielun Hu3Wenwen Su4Zheyu Guo5Yanli Zhang6Shaoping Nie7State Key Laboratory of Food Science and Resources, China‐Canada Joint Lab of Food Science and Technology, Key Laboratory of Bioactive Polysaccharides of Jiangxi Province Nanchang University Nanchang ChinaState Key Laboratory of Food Science and Resources, China‐Canada Joint Lab of Food Science and Technology, Key Laboratory of Bioactive Polysaccharides of Jiangxi Province Nanchang University Nanchang ChinaState Key Laboratory of Food Science and Resources, China‐Canada Joint Lab of Food Science and Technology, Key Laboratory of Bioactive Polysaccharides of Jiangxi Province Nanchang University Nanchang ChinaState Key Laboratory of Food Science and Resources, China‐Canada Joint Lab of Food Science and Technology, Key Laboratory of Bioactive Polysaccharides of Jiangxi Province Nanchang University Nanchang ChinaState Key Laboratory of Food Science and Resources, China‐Canada Joint Lab of Food Science and Technology, Key Laboratory of Bioactive Polysaccharides of Jiangxi Province Nanchang University Nanchang ChinaState Key Laboratory of Food Science and Resources, China‐Canada Joint Lab of Food Science and Technology, Key Laboratory of Bioactive Polysaccharides of Jiangxi Province Nanchang University Nanchang ChinaState Key Laboratory of Food Science and Resources, China‐Canada Joint Lab of Food Science and Technology, Key Laboratory of Bioactive Polysaccharides of Jiangxi Province Nanchang University Nanchang ChinaState Key Laboratory of Food Science and Resources, China‐Canada Joint Lab of Food Science and Technology, Key Laboratory of Bioactive Polysaccharides of Jiangxi Province Nanchang University Nanchang ChinaAbstract Polysaccharides are typically resistant to digestion in the gastrointestinal tract and are instead degraded by gut microbiota in the colon. As such, they are commonly employed as carriers for colon‐targeted drugs, with the potential to regulate gut microbiota. Pectin, carrageenan, guar gum, and xanthan gum are commonly used polysaccharide carriers, but their degradation in the gut microbiota under colitis conditions as well as their effects on gut microbiota remains unclear. In this study, we performed the in vitro fermentation of these four polysaccharides using colonic content microbiota from mice with colitis and evaluated the degree of polysaccharide degradation and the effects on pH, short‐chain fatty acids, and gut microbiota. Our findings indicate that pectin and guar gum had a greater degree of degradation and promoted the production of butyrate, inhibited the proliferation of harmful bacteria (e.g., Enterobacteriaceae), and increased beneficial bacteria (e.g., Bifidobacterium). In contrast, carrageenan and xanthan gum promote the proliferation of Enterobacteriaceae. These results provide theoretical guidance for the selection of drug delivery carriers for inflammatory bowel disease treatment and provide information on the relationship between polysaccharides and gut microbiota.https://doi.org/10.1002/fft2.270gut microbiotain vitro fermentationinflammatory bowel diseasepolysaccharides |
| spellingShingle | Shanshan Zhang Yonggan Sun Qixing Nie Jielun Hu Wenwen Su Zheyu Guo Yanli Zhang Shaoping Nie In vitro assessment of the effect of four polysaccharides on intestinal bacteria of mice with colitis Food Frontiers gut microbiota in vitro fermentation inflammatory bowel disease polysaccharides |
| title | In vitro assessment of the effect of four polysaccharides on intestinal bacteria of mice with colitis |
| title_full | In vitro assessment of the effect of four polysaccharides on intestinal bacteria of mice with colitis |
| title_fullStr | In vitro assessment of the effect of four polysaccharides on intestinal bacteria of mice with colitis |
| title_full_unstemmed | In vitro assessment of the effect of four polysaccharides on intestinal bacteria of mice with colitis |
| title_short | In vitro assessment of the effect of four polysaccharides on intestinal bacteria of mice with colitis |
| title_sort | in vitro assessment of the effect of four polysaccharides on intestinal bacteria of mice with colitis |
| topic | gut microbiota in vitro fermentation inflammatory bowel disease polysaccharides |
| url | https://doi.org/10.1002/fft2.270 |
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