Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants
Phenolic compounds, such as catechol, are released into the environment from a variety of industrial sources and they present a serious ecosystem burden. This work examined the possibility of using partially purified apple polyphenol oxidase (PPO) for bioremediation of phenolic contaminants. In orde...
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| Format: | Article |
| Language: | English |
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Croatian Society of Chemical Engineers
2018-04-01
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| Series: | Kemija u Industriji |
| Subjects: | |
| Online Access: | http://silverstripe.fkit.hr/kui/assets/Uploads/1-109-117.pdf |
| _version_ | 1818525366703095808 |
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| author | Anita Šalić Ivica Strelec Bruno Zelić Marina Tišma |
| author_facet | Anita Šalić Ivica Strelec Bruno Zelić Marina Tišma |
| author_sort | Anita Šalić |
| collection | DOAJ |
| description | Phenolic compounds, such as catechol, are released into the environment from a variety of industrial sources and they present a serious ecosystem burden. This work examined the possibility of using partially purified apple polyphenol oxidase (PPO) for bioremediation of phenolic contaminants. In order to optimize process conditions, the optimal pH and temperature for PPO activity were determined, while PPO affinity toward various phenols, as well as the effect of some salts and organic solvents which can be found in wastewaters, was used to confirm applicability of PPO in wastewater treatment. It was found that partially purified apple PPO shows maximal activity at pH 6.8 and 25 °C, but exhibits more than 85 % of its maximal activity in pH range from 5 to 8, and more than 90 % of activity in temperature range from 10 to 50 °C. PPO showed high affinity for various diphenols, but lack of affinity toward monophenols. Sodium tetraborate decahydrate moderately inhibited PPO activity, while exposure of PPO to the presence of organic solvents (φ = 5 %) caused 40 % loss in its activity. Catechol oxidation by PPO performed for just 5 min in a batch reactor at optimal process conditions resulted in 25 % conversion. Based on obtained data, it seems that partially purified apple PPO has reasonable potential in wastewater treatment. |
| first_indexed | 2024-12-11T06:08:26Z |
| format | Article |
| id | doaj.art-054a31a94d714ffe938570550dcb83a6 |
| institution | Directory Open Access Journal |
| issn | 0022-9830 1334-9090 |
| language | English |
| last_indexed | 2024-12-11T06:08:26Z |
| publishDate | 2018-04-01 |
| publisher | Croatian Society of Chemical Engineers |
| record_format | Article |
| series | Kemija u Industriji |
| spelling | doaj.art-054a31a94d714ffe938570550dcb83a62022-12-22T01:18:12ZengCroatian Society of Chemical EngineersKemija u Industriji0022-98301334-90902018-04-01673-410911610.15255/KUI.2017.036Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic ContaminantsAnita Šalić0Ivica Strelec1Bruno Zelić2Marina Tišma3University of Zagreb, Faculty of Chemical Engineering and Technology, Marulićev trg 19, HR-10 000 Zagreb, CroatiaJosip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, HR-31 000 Osijek, CroatiaUniversity of Zagreb, Faculty of Chemical Engineering and Technology, Marulićev trg 19, HR-10 000 Zagreb, CroatiaJosip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, HR-31 000 Osijek, CroatiaPhenolic compounds, such as catechol, are released into the environment from a variety of industrial sources and they present a serious ecosystem burden. This work examined the possibility of using partially purified apple polyphenol oxidase (PPO) for bioremediation of phenolic contaminants. In order to optimize process conditions, the optimal pH and temperature for PPO activity were determined, while PPO affinity toward various phenols, as well as the effect of some salts and organic solvents which can be found in wastewaters, was used to confirm applicability of PPO in wastewater treatment. It was found that partially purified apple PPO shows maximal activity at pH 6.8 and 25 °C, but exhibits more than 85 % of its maximal activity in pH range from 5 to 8, and more than 90 % of activity in temperature range from 10 to 50 °C. PPO showed high affinity for various diphenols, but lack of affinity toward monophenols. Sodium tetraborate decahydrate moderately inhibited PPO activity, while exposure of PPO to the presence of organic solvents (φ = 5 %) caused 40 % loss in its activity. Catechol oxidation by PPO performed for just 5 min in a batch reactor at optimal process conditions resulted in 25 % conversion. Based on obtained data, it seems that partially purified apple PPO has reasonable potential in wastewater treatment.http://silverstripe.fkit.hr/kui/assets/Uploads/1-109-117.pdfcatechol oxidationenzymatic wastewater treatmentphenolic contaminantspolyphenol oxidase (PPO) |
| spellingShingle | Anita Šalić Ivica Strelec Bruno Zelić Marina Tišma Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants Kemija u Industriji catechol oxidation enzymatic wastewater treatment phenolic contaminants polyphenol oxidase (PPO) |
| title | Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants |
| title_full | Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants |
| title_fullStr | Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants |
| title_full_unstemmed | Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants |
| title_short | Potential Use of Apple Polyphenol Oxidase for Bioremediation of Phenolic Contaminants |
| title_sort | potential use of apple polyphenol oxidase for bioremediation of phenolic contaminants |
| topic | catechol oxidation enzymatic wastewater treatment phenolic contaminants polyphenol oxidase (PPO) |
| url | http://silverstripe.fkit.hr/kui/assets/Uploads/1-109-117.pdf |
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