There Is (Scientific) Strength in Numbers: A Comprehensive Quantitation of Fc Gamma Receptor Numbers on Human and Murine Peripheral Blood Leukocytes

Antibodies are essential mediators of immunological defense mechanisms, are clinically used as therapeutic agents, but are also functionally involved in various immune-mediated disorders. Whereas IgG antibodies accomplish some of their biological tasks autonomously, many functions depend on their bi...

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Main Authors: Christina Kerntke, Falk Nimmerjahn, Markus Biburger
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-02-01
Series:Frontiers in Immunology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2020.00118/full
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author Christina Kerntke
Falk Nimmerjahn
Falk Nimmerjahn
Markus Biburger
Markus Biburger
author_facet Christina Kerntke
Falk Nimmerjahn
Falk Nimmerjahn
Markus Biburger
Markus Biburger
author_sort Christina Kerntke
collection DOAJ
description Antibodies are essential mediators of immunological defense mechanisms, are clinically used as therapeutic agents, but are also functionally involved in various immune-mediated disorders. Whereas IgG antibodies accomplish some of their biological tasks autonomously, many functions depend on their binding to activating and inhibitory Fcγ receptors (FcγR). From a qualitative point of view expression patterns of FcγR on immunologically relevant cell types are well-characterized both for mice and humans. Surprisingly, however, there is only quite limited information available on actual quantities of FcγR expressed by the different leukocyte populations. In this study we provide a comprehensive data set assessing quantitatively how many individual human and mouse FcγRs are expressed on B cells, NK cells, eosinophils, neutrophils, basophils and both classical, and non-classical monocytes under steady state conditions. Moreover, among human donors we found two groups with different expression levels of the inhibitory FcγRIIb on monocytes which appears to correlate with haplotypes of the activating FcγRIIIa.
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spelling doaj.art-054d3343aa72473e998c08b6660b38562022-12-21T23:41:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-02-011110.3389/fimmu.2020.00118489401There Is (Scientific) Strength in Numbers: A Comprehensive Quantitation of Fc Gamma Receptor Numbers on Human and Murine Peripheral Blood LeukocytesChristina Kerntke0Falk Nimmerjahn1Falk Nimmerjahn2Markus Biburger3Markus Biburger4Division of Genetics, Department of Biology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, GermanyDivision of Genetics, Department of Biology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, GermanyMedical Immunology Campus Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, GermanyDivision of Genetics, Department of Biology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, GermanyMedical Immunology Campus Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, GermanyAntibodies are essential mediators of immunological defense mechanisms, are clinically used as therapeutic agents, but are also functionally involved in various immune-mediated disorders. Whereas IgG antibodies accomplish some of their biological tasks autonomously, many functions depend on their binding to activating and inhibitory Fcγ receptors (FcγR). From a qualitative point of view expression patterns of FcγR on immunologically relevant cell types are well-characterized both for mice and humans. Surprisingly, however, there is only quite limited information available on actual quantities of FcγR expressed by the different leukocyte populations. In this study we provide a comprehensive data set assessing quantitatively how many individual human and mouse FcγRs are expressed on B cells, NK cells, eosinophils, neutrophils, basophils and both classical, and non-classical monocytes under steady state conditions. Moreover, among human donors we found two groups with different expression levels of the inhibitory FcγRIIb on monocytes which appears to correlate with haplotypes of the activating FcγRIIIa.https://www.frontiersin.org/article/10.3389/fimmu.2020.00118/fullFc receptorsantibodieshuman leukocytesmurine leukocytesquantificationreceptor numbers
spellingShingle Christina Kerntke
Falk Nimmerjahn
Falk Nimmerjahn
Markus Biburger
Markus Biburger
There Is (Scientific) Strength in Numbers: A Comprehensive Quantitation of Fc Gamma Receptor Numbers on Human and Murine Peripheral Blood Leukocytes
Frontiers in Immunology
Fc receptors
antibodies
human leukocytes
murine leukocytes
quantification
receptor numbers
title There Is (Scientific) Strength in Numbers: A Comprehensive Quantitation of Fc Gamma Receptor Numbers on Human and Murine Peripheral Blood Leukocytes
title_full There Is (Scientific) Strength in Numbers: A Comprehensive Quantitation of Fc Gamma Receptor Numbers on Human and Murine Peripheral Blood Leukocytes
title_fullStr There Is (Scientific) Strength in Numbers: A Comprehensive Quantitation of Fc Gamma Receptor Numbers on Human and Murine Peripheral Blood Leukocytes
title_full_unstemmed There Is (Scientific) Strength in Numbers: A Comprehensive Quantitation of Fc Gamma Receptor Numbers on Human and Murine Peripheral Blood Leukocytes
title_short There Is (Scientific) Strength in Numbers: A Comprehensive Quantitation of Fc Gamma Receptor Numbers on Human and Murine Peripheral Blood Leukocytes
title_sort there is scientific strength in numbers a comprehensive quantitation of fc gamma receptor numbers on human and murine peripheral blood leukocytes
topic Fc receptors
antibodies
human leukocytes
murine leukocytes
quantification
receptor numbers
url https://www.frontiersin.org/article/10.3389/fimmu.2020.00118/full
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