Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red Meats
Abstract Background and Objective: Listeria monocytogenes is a bacterium transferred by foods and is the agent of many sporadic and epidemic diseases in humans. This study aimed to investigate the prevalence of L. monocytogenes and to determinine their antibiotic resistance profile in red meats....
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| Format: | Article |
| Language: | English |
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Golestan University of Medical Sciences
2015-01-01
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| Series: | Medical Laboratory Journal |
| Subjects: | |
| Online Access: | http://mlj.goums.ac.ir/article-1-533-en.html |
| _version_ | 1819077997421920256 |
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| author | Kargar, M Ebrahimi, E Amini, J Najafi, A Kheirkhah, B |
| author_facet | Kargar, M Ebrahimi, E Amini, J Najafi, A Kheirkhah, B |
| author_sort | Kargar, M |
| collection | DOAJ |
| description | Abstract
Background and Objective: Listeria monocytogenes is a bacterium transferred by foods and is the agent of many sporadic and epidemic diseases in humans. This study aimed to investigate the prevalence of L. monocytogenes and to determinine their antibiotic resistance profile in red meats.
Material and Methods: this cross-sectional study was performed on 400 red meat samples obtained from industrial slaughterhouses placed in Kerman, Iran. First, the samples were enriched with Simultaneous Enrichment Broth (SEB), and then plated onto Palcam agar and Tryptic Soy Broth Yeast Extract Broth (TSAYE). After identification of the isolates based on biochemical tests and PCR, the isolates were checked for their antibiotic resistance profile using disk Diffusion
Results: of 400 samples, 12 samples (3%) were contaminated with different species of Listeria. Using PCR, hly gene was recognized in eight samples (2%) of L. monocytogenes. Furthermore, there was a significant difference in isolation rate of lamb samples compared to cow ones. While all of the isolates were resistant to clindamycin, amikacin and chloramphenicol, they were sensitive to penicillin.
Conclusion: in spite of low rate of infection in red meat samples in Kerman city, due to high risk of Listeria contamination in red meats, we recommend applying a routine screening to identify this bacterium in our county.
Keywords: Listeria Monocytogenes, Hly Gene, Red Meat, Antibiotic, Kerman |
| first_indexed | 2024-12-21T19:06:05Z |
| format | Article |
| id | doaj.art-054e87b16efc45ca9f679ae476e1ad37 |
| institution | Directory Open Access Journal |
| issn | 2538-4449 |
| language | English |
| last_indexed | 2024-12-21T19:06:05Z |
| publishDate | 2015-01-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj.art-054e87b16efc45ca9f679ae476e1ad372022-12-21T18:53:20ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492015-01-01842733Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red MeatsKargar, M0Ebrahimi, E1Amini, J2Najafi, A3Kheirkhah, B4 Jahrom Branch, Islamic Azad University, Jahrom, Iran. Islamic Azad University, Jahrom Branch, Iran Islamic Azad University, Kerman Branch, Iran. The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran Islamic Azad University, Kerman Branch, Iran Abstract Background and Objective: Listeria monocytogenes is a bacterium transferred by foods and is the agent of many sporadic and epidemic diseases in humans. This study aimed to investigate the prevalence of L. monocytogenes and to determinine their antibiotic resistance profile in red meats. Material and Methods: this cross-sectional study was performed on 400 red meat samples obtained from industrial slaughterhouses placed in Kerman, Iran. First, the samples were enriched with Simultaneous Enrichment Broth (SEB), and then plated onto Palcam agar and Tryptic Soy Broth Yeast Extract Broth (TSAYE). After identification of the isolates based on biochemical tests and PCR, the isolates were checked for their antibiotic resistance profile using disk Diffusion Results: of 400 samples, 12 samples (3%) were contaminated with different species of Listeria. Using PCR, hly gene was recognized in eight samples (2%) of L. monocytogenes. Furthermore, there was a significant difference in isolation rate of lamb samples compared to cow ones. While all of the isolates were resistant to clindamycin, amikacin and chloramphenicol, they were sensitive to penicillin. Conclusion: in spite of low rate of infection in red meat samples in Kerman city, due to high risk of Listeria contamination in red meats, we recommend applying a routine screening to identify this bacterium in our county. Keywords: Listeria Monocytogenes, Hly Gene, Red Meat, Antibiotic, Kermanhttp://mlj.goums.ac.ir/article-1-533-en.htmllisteria monocytogeneshly genered meatantibiotickerman |
| spellingShingle | Kargar, M Ebrahimi, E Amini, J Najafi, A Kheirkhah, B Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red Meats Medical Laboratory Journal listeria monocytogenes hly gene red meat antibiotic kerman |
| title | Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red Meats |
| title_full | Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red Meats |
| title_fullStr | Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red Meats |
| title_full_unstemmed | Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red Meats |
| title_short | Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red Meats |
| title_sort | isolation identification and antibiotic resistance profile of listeria monocytogenes strains in red meats |
| topic | listeria monocytogenes hly gene red meat antibiotic kerman |
| url | http://mlj.goums.ac.ir/article-1-533-en.html |
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