| Tóm tắt: | Over the past ten years, particular attention has been paid to <i>Artemisia</i> L. genus plants and their chemical composition. <i>Artemisia abrotanum</i> L. and <i>Artemisia absinthium</i> L., which are traditional medicinal, aromatic plants with a strong digestive activity that is determined by the various phytochemicals. The research aim was to determine the variation in polyphenols in the samples of different vegetation stages of two <i>Artemisia</i> species and to evaluate the antioxidant activity of methanolic extracts in vitro. Phytochemical analysis of the <i>Artemisia</i> samples was carried out using spectrophotometric methods and HPLC-PDA techniques, while antioxidant activity was determined using DPPH, ABTS, FRAP, and CUPRAC assays. Significant differences in the diversity of phenolic compounds were found among <i>Artemisia</i> species during vegetation stages. Chlorogenic acid, 3,5-dicaffeoylquinic acid, and rutin were predominant among the phenolic compounds. The study provides valuable insights into the composition of phenolic compounds and flavonoids in <i>Artemisia</i> plants at different growth stages, shedding light on their potential pharmacological effects and antioxidant activities. These findings contribute to the knowledge of the quality and safety of herbal materials derived from <i>Artemisia</i> species.
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