Expression and Prognostic Role of Glia Maturation Factor-γ in Gliomas
BackgroundGlia maturation factor-γ (GMFG) regulates actin cytoskeletal organization and promotes the invasion of cancer cells. However, its expression pattern and molecular function in gliomas have not been clearly defined.MethodsIn this study, public datasets comprising 2,518 gliomas samples were u...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2022.906762/full |
| _version_ | 1818547203142057984 |
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| author | Junhui Liu Junhui Liu Xiaonan Zhu Lun Gao Rongxin Geng Xiang Tao Haitao Xu Zhibiao Chen |
| author_facet | Junhui Liu Junhui Liu Xiaonan Zhu Lun Gao Rongxin Geng Xiang Tao Haitao Xu Zhibiao Chen |
| author_sort | Junhui Liu |
| collection | DOAJ |
| description | BackgroundGlia maturation factor-γ (GMFG) regulates actin cytoskeletal organization and promotes the invasion of cancer cells. However, its expression pattern and molecular function in gliomas have not been clearly defined.MethodsIn this study, public datasets comprising 2,518 gliomas samples were used to explore GMFG expression and its correlation with malignancy in gliomas. Immunohistochemistry (IHC) staining was performed to determine the expression of GMFG in gliomas using an in-house cohort that contained 120 gliomas samples. Gene ontology enrichment analysis was conducted using the DAVID tool. The correlation between GMFG expression and immune cell infiltration was evaluated using TIMER, Tumor Immune Single-Cell Hub (TISCH) database, and IHC staining assays. The Kaplan–Meier analysis was performed to determine the prognostic role of GMFG and its association with temozolomide (TMZ) response in gliomas.ResultsThe GMFG expression was higher in gliomas compared with non-tumor brain tissues both in public datasets and in-house cohort. High expression of GMFG was significantly associated with WHO grade IV, IDH 1/2 wild-type, and mesenchymal (ME) subtypes. Bioinformatic prediction and IHC analysis revealed that GMFG expression obviously correlated with the macrophage marker CD163 in gliomas. Moreover, both lower grade glioma (LGG) and glioblastoma multiforme (GBM) patients with high GMFG expression had shorter overall survival than those with low GMFG expression. These results indicate that GMFG may be a therapeutic target for the treatment of such patients. Patients with low GMFG expression who received chemotherapy had a longer survival time than those with high GMFG expression. For patients who received ion radiotherapy (IR) only, the GMFG expression level had no effect on the overall survival neither in CGGA and TCGA datasets.ConclusionThe GMFG is a novel prognostic biomarker for patients with both LGG and GBM. Increased GMFG expression is associated with tumor-associated macrophages (TAMs) infiltration and with a bad response to TMZ treatment. |
| first_indexed | 2024-12-12T08:03:34Z |
| format | Article |
| id | doaj.art-05533814b62f43f3b1f457c04c605a61 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-12T08:03:34Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-05533814b62f43f3b1f457c04c605a612022-12-22T00:32:02ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992022-06-011510.3389/fnmol.2022.906762906762Expression and Prognostic Role of Glia Maturation Factor-γ in GliomasJunhui Liu0Junhui Liu1Xiaonan Zhu2Lun Gao3Rongxin Geng4Xiang Tao5Haitao Xu6Zhibiao Chen7Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, ChinaBackgroundGlia maturation factor-γ (GMFG) regulates actin cytoskeletal organization and promotes the invasion of cancer cells. However, its expression pattern and molecular function in gliomas have not been clearly defined.MethodsIn this study, public datasets comprising 2,518 gliomas samples were used to explore GMFG expression and its correlation with malignancy in gliomas. Immunohistochemistry (IHC) staining was performed to determine the expression of GMFG in gliomas using an in-house cohort that contained 120 gliomas samples. Gene ontology enrichment analysis was conducted using the DAVID tool. The correlation between GMFG expression and immune cell infiltration was evaluated using TIMER, Tumor Immune Single-Cell Hub (TISCH) database, and IHC staining assays. The Kaplan–Meier analysis was performed to determine the prognostic role of GMFG and its association with temozolomide (TMZ) response in gliomas.ResultsThe GMFG expression was higher in gliomas compared with non-tumor brain tissues both in public datasets and in-house cohort. High expression of GMFG was significantly associated with WHO grade IV, IDH 1/2 wild-type, and mesenchymal (ME) subtypes. Bioinformatic prediction and IHC analysis revealed that GMFG expression obviously correlated with the macrophage marker CD163 in gliomas. Moreover, both lower grade glioma (LGG) and glioblastoma multiforme (GBM) patients with high GMFG expression had shorter overall survival than those with low GMFG expression. These results indicate that GMFG may be a therapeutic target for the treatment of such patients. Patients with low GMFG expression who received chemotherapy had a longer survival time than those with high GMFG expression. For patients who received ion radiotherapy (IR) only, the GMFG expression level had no effect on the overall survival neither in CGGA and TCGA datasets.ConclusionThe GMFG is a novel prognostic biomarker for patients with both LGG and GBM. Increased GMFG expression is associated with tumor-associated macrophages (TAMs) infiltration and with a bad response to TMZ treatment.https://www.frontiersin.org/articles/10.3389/fnmol.2022.906762/fullGMFGgliomasprognosisimmunetemozolomide |
| spellingShingle | Junhui Liu Junhui Liu Xiaonan Zhu Lun Gao Rongxin Geng Xiang Tao Haitao Xu Zhibiao Chen Expression and Prognostic Role of Glia Maturation Factor-γ in Gliomas Frontiers in Molecular Neuroscience GMFG gliomas prognosis immune temozolomide |
| title | Expression and Prognostic Role of Glia Maturation Factor-γ in Gliomas |
| title_full | Expression and Prognostic Role of Glia Maturation Factor-γ in Gliomas |
| title_fullStr | Expression and Prognostic Role of Glia Maturation Factor-γ in Gliomas |
| title_full_unstemmed | Expression and Prognostic Role of Glia Maturation Factor-γ in Gliomas |
| title_short | Expression and Prognostic Role of Glia Maturation Factor-γ in Gliomas |
| title_sort | expression and prognostic role of glia maturation factor γ in gliomas |
| topic | GMFG gliomas prognosis immune temozolomide |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2022.906762/full |
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