β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential
Abstract Microbial β-glucosidases can be used in several industrial processes, including production of biofuels, functional foods, juices, and beverages. In the present work, production of β-glucosidase by solid state cultivation of the fungus Thermoascus crustaceus in a low-cost cultivation medium...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2021-03-01
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| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/aabc/v93n1/0001-3765-aabc-93-01-e20191349.pdf |
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| author | ANDREZA P. GARBIN NAYARA F.L. GARCIA GABRIELA F. CAVALHEIRO MARIA ALICE SILVESTRE ANDRÉ RODRIGUES MARCELO F. DA PAZ GUSTAVO G. FONSECA RODRIGO S.R. LEITE |
| author_facet | ANDREZA P. GARBIN NAYARA F.L. GARCIA GABRIELA F. CAVALHEIRO MARIA ALICE SILVESTRE ANDRÉ RODRIGUES MARCELO F. DA PAZ GUSTAVO G. FONSECA RODRIGO S.R. LEITE |
| author_sort | ANDREZA P. GARBIN |
| collection | DOAJ |
| description | Abstract Microbial β-glucosidases can be used in several industrial processes, including production of biofuels, functional foods, juices, and beverages. In the present work, production of β-glucosidase by solid state cultivation of the fungus Thermoascus crustaceus in a low-cost cultivation medium (comprising agroindustrial residues) was evaluated. The highest production of β-glucosidase, about 415.1 U/g substrate (or 41.51 U/mL), was obtained by cultivating the fungus in wheat bran with 70% humidity, during 96 h at 40°C. The enzymatic activity was optimum at pH 4.5 and 65°C. β-Glucosidase maintained its catalytic activity when incubated at a pH range of 4.0-8.0 and temperature of 30-55°C. The enzyme was strongly inhibited by glucose; even when the substrate and glucose concentrations were equal, the inhibition was not reversed, suggesting a non-competitive inhibition. In the presence of up to 10% ethanol, β-glucosidase maintained its catalytic activity. In addition to β-glucosidase, the enzymatic extract showed activity of 36 U/g for endoglucanase, 256.2 U/g for xylanase, and 18.2 U/g for β-xylosidase. The results allow to conclude that the fungus T. crustaceus has considerable potential for production of β-glucosidase and xylanase when cultivated in agroindustrial residues, thereby reducing the cost of these biocatalysts. |
| first_indexed | 2024-04-11T18:33:52Z |
| format | Article |
| id | doaj.art-05542b5e60e84404814fb224fb55025a |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-11T18:33:52Z |
| publishDate | 2021-03-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-05542b5e60e84404814fb224fb55025a2022-12-22T04:09:20ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902021-03-0193110.1590/0001-3765202120191349β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potentialANDREZA P. GARBINhttps://orcid.org/0000-0002-4140-3012NAYARA F.L. GARCIAhttps://orcid.org/0000-0002-6038-0047GABRIELA F. CAVALHEIROhttps://orcid.org/0000-0002-7227-6677MARIA ALICE SILVESTREANDRÉ RODRIGUEShttps://orcid.org/0000-0002-4164-9362MARCELO F. DA PAZhttps://orcid.org/0000-0002-5176-2895GUSTAVO G. FONSECAhttps://orcid.org/0000-0002-8784-661XRODRIGO S.R. LEITEhttps://orcid.org/0000-0002-0837-5072Abstract Microbial β-glucosidases can be used in several industrial processes, including production of biofuels, functional foods, juices, and beverages. In the present work, production of β-glucosidase by solid state cultivation of the fungus Thermoascus crustaceus in a low-cost cultivation medium (comprising agroindustrial residues) was evaluated. The highest production of β-glucosidase, about 415.1 U/g substrate (or 41.51 U/mL), was obtained by cultivating the fungus in wheat bran with 70% humidity, during 96 h at 40°C. The enzymatic activity was optimum at pH 4.5 and 65°C. β-Glucosidase maintained its catalytic activity when incubated at a pH range of 4.0-8.0 and temperature of 30-55°C. The enzyme was strongly inhibited by glucose; even when the substrate and glucose concentrations were equal, the inhibition was not reversed, suggesting a non-competitive inhibition. In the presence of up to 10% ethanol, β-glucosidase maintained its catalytic activity. In addition to β-glucosidase, the enzymatic extract showed activity of 36 U/g for endoglucanase, 256.2 U/g for xylanase, and 18.2 U/g for β-xylosidase. The results allow to conclude that the fungus T. crustaceus has considerable potential for production of β-glucosidase and xylanase when cultivated in agroindustrial residues, thereby reducing the cost of these biocatalysts.http://www.scielo.br/pdf/aabc/v93n1/0001-3765-aabc-93-01-e20191349.pdfCellulasesfilamentous fungusindustrial enzymessolid state cultivationxylanases |
| spellingShingle | ANDREZA P. GARBIN NAYARA F.L. GARCIA GABRIELA F. CAVALHEIRO MARIA ALICE SILVESTRE ANDRÉ RODRIGUES MARCELO F. DA PAZ GUSTAVO G. FONSECA RODRIGO S.R. LEITE β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential Anais da Academia Brasileira de Ciências Cellulases filamentous fungus industrial enzymes solid state cultivation xylanases |
| title | β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential |
| title_full | β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential |
| title_fullStr | β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential |
| title_full_unstemmed | β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential |
| title_short | β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential |
| title_sort | β glucosidase from thermophilic fungus thermoascus crustaceus production and industrial potential |
| topic | Cellulases filamentous fungus industrial enzymes solid state cultivation xylanases |
| url | http://www.scielo.br/pdf/aabc/v93n1/0001-3765-aabc-93-01-e20191349.pdf |
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