Biocontrol and Rapid Detection of Food-borne Pathogens Using Bacteriophages and Endolysins
Bacteriophages have been suggested as natural food preservatives as well as rapid detection materials for food-borne pathogens in various foods. Since Listeria monocytogenes-targeting phage cocktail (ListShield) was approved for applications in foods, numerous phages have been screened and experimen...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00474/full |
| _version_ | 1828769657782272000 |
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| author | Jaewoo eBai You-Tae eKim Sangryeol eRyu Sangryeol eRyu Ju-Hoon eLee |
| author_facet | Jaewoo eBai You-Tae eKim Sangryeol eRyu Sangryeol eRyu Ju-Hoon eLee |
| author_sort | Jaewoo eBai |
| collection | DOAJ |
| description | Bacteriophages have been suggested as natural food preservatives as well as rapid detection materials for food-borne pathogens in various foods. Since Listeria monocytogenes-targeting phage cocktail (ListShield) was approved for applications in foods, numerous phages have been screened and experimentally characterized for phage applications in foods. A single phage and phage cocktail treatments to various foods contaminated with food-borne pathogens including E. coli O157:H7, Salmonella enterica, Campylobacter jejuni, Listeria monocytogenes, Staphylococcus aureus, Cronobacter sakazakii, and Vibrio spp. revealed that they have great potential to control various food-borne pathogens and may be alternative for conventional food preservatives. In addition, phage-derived endolysins with high host specificity and host lysis activities may be preferred to food applications rather than phages. For rapid detection of food-borne pathogens, cell-wall binding domains (CBDs) from endolysins have been suggested due to their high host-specific binding. Fluorescence-tagged CBDs have been successfully evaluated and suggested to be alternative materials of expensive antibodies for various detection applications. Most recently, reporter phage systems have been developed and tested to confirm their usability and accuracy for specific detection. These systems revealed some advantages like rapid detection of only viable pathogenic cells without interference by food components in a very short reaction time, suggesting that these systems may be suitable for monitoring of pathogens in foods. Consequently, phage is the next-generation biocontrol agent as well as rapid detection tool to confirm and even identify the food-borne pathogens present in various foods. |
| first_indexed | 2024-12-11T13:52:03Z |
| format | Article |
| id | doaj.art-055490e1686c4569a0d92f8a68eb6410 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T13:52:03Z |
| publishDate | 2016-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-055490e1686c4569a0d92f8a68eb64102022-12-22T01:04:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-04-01710.3389/fmicb.2016.00474189888Biocontrol and Rapid Detection of Food-borne Pathogens Using Bacteriophages and EndolysinsJaewoo eBai0You-Tae eKim1Sangryeol eRyu2Sangryeol eRyu3Ju-Hoon eLee4Seoul National UniversityKyung Hee UniversitySeoul National UniversitySeoul National UniversityKyung Hee UniversityBacteriophages have been suggested as natural food preservatives as well as rapid detection materials for food-borne pathogens in various foods. Since Listeria monocytogenes-targeting phage cocktail (ListShield) was approved for applications in foods, numerous phages have been screened and experimentally characterized for phage applications in foods. A single phage and phage cocktail treatments to various foods contaminated with food-borne pathogens including E. coli O157:H7, Salmonella enterica, Campylobacter jejuni, Listeria monocytogenes, Staphylococcus aureus, Cronobacter sakazakii, and Vibrio spp. revealed that they have great potential to control various food-borne pathogens and may be alternative for conventional food preservatives. In addition, phage-derived endolysins with high host specificity and host lysis activities may be preferred to food applications rather than phages. For rapid detection of food-borne pathogens, cell-wall binding domains (CBDs) from endolysins have been suggested due to their high host-specific binding. Fluorescence-tagged CBDs have been successfully evaluated and suggested to be alternative materials of expensive antibodies for various detection applications. Most recently, reporter phage systems have been developed and tested to confirm their usability and accuracy for specific detection. These systems revealed some advantages like rapid detection of only viable pathogenic cells without interference by food components in a very short reaction time, suggesting that these systems may be suitable for monitoring of pathogens in foods. Consequently, phage is the next-generation biocontrol agent as well as rapid detection tool to confirm and even identify the food-borne pathogens present in various foods.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00474/fullFood PreservativesBacteriophagebiocontrolFood-borne pathogenscell-wall binding domainendolysin |
| spellingShingle | Jaewoo eBai You-Tae eKim Sangryeol eRyu Sangryeol eRyu Ju-Hoon eLee Biocontrol and Rapid Detection of Food-borne Pathogens Using Bacteriophages and Endolysins Frontiers in Microbiology Food Preservatives Bacteriophage biocontrol Food-borne pathogens cell-wall binding domain endolysin |
| title | Biocontrol and Rapid Detection of Food-borne Pathogens Using Bacteriophages and Endolysins |
| title_full | Biocontrol and Rapid Detection of Food-borne Pathogens Using Bacteriophages and Endolysins |
| title_fullStr | Biocontrol and Rapid Detection of Food-borne Pathogens Using Bacteriophages and Endolysins |
| title_full_unstemmed | Biocontrol and Rapid Detection of Food-borne Pathogens Using Bacteriophages and Endolysins |
| title_short | Biocontrol and Rapid Detection of Food-borne Pathogens Using Bacteriophages and Endolysins |
| title_sort | biocontrol and rapid detection of food borne pathogens using bacteriophages and endolysins |
| topic | Food Preservatives Bacteriophage biocontrol Food-borne pathogens cell-wall binding domain endolysin |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00474/full |
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