Survey of Viruses Present in Radish Fields in 2014
A 2014 nationwide survey in radish fields investigated the distribution of common viruses and possible emerging viruses. Radish leaves with virus-like symptoms were collected and 108 samples assayed by RT-PCR using specific primers for Radish mosaic virus (RaMV), Cucumber mosaic virus (CMV), and Tur...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2015-09-01
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| Series: | Research in Plant Disease |
| Subjects: | |
| Online Access: | http://www.online-rpd.org/journal/view.html?uid=1460&&vmd=Full |
| _version_ | 1818235812325621760 |
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| author | Jinsoo Chung Jae-Yeong Han Jungkyu Kim Hyekyoung Ju Junsu Gong John Hammond Hyoun-Sub Lim |
| author_facet | Jinsoo Chung Jae-Yeong Han Jungkyu Kim Hyekyoung Ju Junsu Gong John Hammond Hyoun-Sub Lim |
| author_sort | Jinsoo Chung |
| collection | DOAJ |
| description | A 2014 nationwide survey in radish fields investigated the distribution of common viruses and possible emerging viruses. Radish leaves with virus-like symptoms were collected and 108 samples assayed by RT-PCR using specific primers for Radish mosaic virus (RaMV), Cucumber mosaic virus (CMV), and Turnip mosaic virus (TuMV); 47 samples were TuMV positive, and RaMV and CMV were detected in 3 and 2 samples, respectively. No samples showed double infection of TuMV/RaMV, or RaMV/CMV, but two double infections of TuMV/CMV were detected. TuMV isolates were sorted by symptom severity, and three isolates (R007-mild; R041 and R065-severe) selected for BLAST and phylogenetic analysis, which indicated that the coat protein (CP) of these isolates (R007, R041, and R065) have approx. 98-99% homology to a previously reported TuMV isolate. RaMV CP showed approx. 99% homology to a previously reported isolate, and the CMV CP is identical to a previously reported Korean isolate (GenBank : GU327368). Three isolates of TuMV showing different pathogenicity (degree of symptom severity) will be valuable to study determinants of pathogenicity. |
| first_indexed | 2024-12-12T11:59:55Z |
| format | Article |
| id | doaj.art-05577c8953f742b1a7a0631f568298f5 |
| institution | Directory Open Access Journal |
| issn | 1598-2262 2233-9191 |
| language | English |
| last_indexed | 2024-12-12T11:59:55Z |
| publishDate | 2015-09-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | Research in Plant Disease |
| spelling | doaj.art-05577c8953f742b1a7a0631f568298f52022-12-22T00:25:07ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622233-91912015-09-01213235242http://dx.doi.org/10.5423/RPD.2015.21.3.235Survey of Viruses Present in Radish Fields in 2014Jinsoo Chung0Jae-Yeong Han1Jungkyu Kim2Hyekyoung Ju3Junsu Gong4John Hammond5 Hyoun-Sub Lim6Chungnam National University, Daejeon 305-764, KoreaChungnam National University, Daejeon 305-764, KoreaChungnam National University, Daejeon 305-764, KoreaChungnam National University, Daejeon 305-764, KoreaChungnam National University, Daejeon 305-764, KoreaFloral and Nursery Plants Research Unit, Beltsville, Maryland, 20705, USAChungnam National University, Daejeon 305-764, KoreaA 2014 nationwide survey in radish fields investigated the distribution of common viruses and possible emerging viruses. Radish leaves with virus-like symptoms were collected and 108 samples assayed by RT-PCR using specific primers for Radish mosaic virus (RaMV), Cucumber mosaic virus (CMV), and Turnip mosaic virus (TuMV); 47 samples were TuMV positive, and RaMV and CMV were detected in 3 and 2 samples, respectively. No samples showed double infection of TuMV/RaMV, or RaMV/CMV, but two double infections of TuMV/CMV were detected. TuMV isolates were sorted by symptom severity, and three isolates (R007-mild; R041 and R065-severe) selected for BLAST and phylogenetic analysis, which indicated that the coat protein (CP) of these isolates (R007, R041, and R065) have approx. 98-99% homology to a previously reported TuMV isolate. RaMV CP showed approx. 99% homology to a previously reported isolate, and the CMV CP is identical to a previously reported Korean isolate (GenBank : GU327368). Three isolates of TuMV showing different pathogenicity (degree of symptom severity) will be valuable to study determinants of pathogenicity.http://www.online-rpd.org/journal/view.html?uid=1460&&vmd=FullCucumber mosaic virusRadish fieldRadish mosaic virus |
| spellingShingle | Jinsoo Chung Jae-Yeong Han Jungkyu Kim Hyekyoung Ju Junsu Gong John Hammond Hyoun-Sub Lim Survey of Viruses Present in Radish Fields in 2014 Research in Plant Disease Cucumber mosaic virus Radish field Radish mosaic virus |
| title | Survey of Viruses Present in Radish Fields in 2014 |
| title_full | Survey of Viruses Present in Radish Fields in 2014 |
| title_fullStr | Survey of Viruses Present in Radish Fields in 2014 |
| title_full_unstemmed | Survey of Viruses Present in Radish Fields in 2014 |
| title_short | Survey of Viruses Present in Radish Fields in 2014 |
| title_sort | survey of viruses present in radish fields in 2014 |
| topic | Cucumber mosaic virus Radish field Radish mosaic virus |
| url | http://www.online-rpd.org/journal/view.html?uid=1460&&vmd=Full |
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