Effect of various co-culture systems on embryo development in ovine
Considering the advent of mesenchymal stem cells (MSCs) as a new source of somatic cells in embryo co-culture system, the current study was aimed to compare in vitro embryo development using embryonic MSCs monolayer with embryonic fibroblast cells (EFCs), oviductal epithelial cells (OECs), and cell-...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2013-10-01
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| Series: | Czech Journal of Animal Science |
| Subjects: | |
| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201310-0002_effect-of-various-co-culture-systems-on-embryo-development-in-ovine.php |
| _version_ | 1797899240847966208 |
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| author | B. Heidari A. Shirazi M.-M. Naderi M.-M. Akhondi H. Hassanpour A. Sarvari S. Borjian |
| author_facet | B. Heidari A. Shirazi M.-M. Naderi M.-M. Akhondi H. Hassanpour A. Sarvari S. Borjian |
| author_sort | B. Heidari |
| collection | DOAJ |
| description | Considering the advent of mesenchymal stem cells (MSCs) as a new source of somatic cells in embryo co-culture system, the current study was aimed to compare in vitro embryo development using embryonic MSCs monolayer with embryonic fibroblast cells (EFCs), oviductal epithelial cells (OECs), and cell-free culture system. The IVM/IVF presumptive sheep zygotes were randomly cultured in different culture conditions as follows: (1) SOFaaBSA medium for the whole culture period (SOF, n = 371), (2) SOFaaBSA medium for the first 3 days followed by co-culturing with MSCs for the next 5 days (SOF-MSCs, n = 120), (3) co-culturing with MSCs for the first 3 days followed by culture in SOFaaBSA medium for the next 5 days (MSCs-SOF, n = 133), (4) co-culturing with MSCs for the whole culture period (MSCs, n = 212), (5) SOFaaBSA medium for the first 3 days followed by co-culturing with EFCs for the next 5 days (SOF-EFCs, n = 132), (6) co-culturing with EFCs for the first 3 days followed by culture in SOFaaBSA medium for the next 5 days (EFCs-SOF, n = 165), (7) co-culturing with EFCs for the whole culture period (EFCs, n = 236), and (8) co-culturing with OECs for the whole culture period (OECs, n = 255). One-Way ANOVA by multiple pairwise comparisons using Tukey's test was performed. Co-culturing in MSCs group had no superiority over EFCs and OECs groups. Though, when co-culturing with MSCs and EFCs was limited to the first 3 days of culture, the embryo development indices were improved compared to the other co-cultured groups. Considering both the hatching rate and total cell number, the application of MSCs for the first 3 days of culture (MSCs-SOF) was superior to the other co-culture and SOF groups. |
| first_indexed | 2024-04-10T08:25:42Z |
| format | Article |
| id | doaj.art-055812a6e87749d3a964ad908f5dac15 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:25:42Z |
| publishDate | 2013-10-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-055812a6e87749d3a964ad908f5dac152023-02-23T03:33:06ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092013-10-01581044345210.17221/6993-CJAScjs-201310-0002Effect of various co-culture systems on embryo development in ovineB. Heidari0A. Shirazi1M.-M. Naderi2M.-M. Akhondi3H. Hassanpour4A. Sarvari5S. Borjian6Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranDepartment of Gametes and Cloning, Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, IranReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranConsidering the advent of mesenchymal stem cells (MSCs) as a new source of somatic cells in embryo co-culture system, the current study was aimed to compare in vitro embryo development using embryonic MSCs monolayer with embryonic fibroblast cells (EFCs), oviductal epithelial cells (OECs), and cell-free culture system. The IVM/IVF presumptive sheep zygotes were randomly cultured in different culture conditions as follows: (1) SOFaaBSA medium for the whole culture period (SOF, n = 371), (2) SOFaaBSA medium for the first 3 days followed by co-culturing with MSCs for the next 5 days (SOF-MSCs, n = 120), (3) co-culturing with MSCs for the first 3 days followed by culture in SOFaaBSA medium for the next 5 days (MSCs-SOF, n = 133), (4) co-culturing with MSCs for the whole culture period (MSCs, n = 212), (5) SOFaaBSA medium for the first 3 days followed by co-culturing with EFCs for the next 5 days (SOF-EFCs, n = 132), (6) co-culturing with EFCs for the first 3 days followed by culture in SOFaaBSA medium for the next 5 days (EFCs-SOF, n = 165), (7) co-culturing with EFCs for the whole culture period (EFCs, n = 236), and (8) co-culturing with OECs for the whole culture period (OECs, n = 255). One-Way ANOVA by multiple pairwise comparisons using Tukey's test was performed. Co-culturing in MSCs group had no superiority over EFCs and OECs groups. Though, when co-culturing with MSCs and EFCs was limited to the first 3 days of culture, the embryo development indices were improved compared to the other co-cultured groups. Considering both the hatching rate and total cell number, the application of MSCs for the first 3 days of culture (MSCs-SOF) was superior to the other co-culture and SOF groups.https://cjas.agriculturejournals.cz/artkey/cjs-201310-0002_effect-of-various-co-culture-systems-on-embryo-development-in-ovine.phpmesenchymal stem cellfibroblastoviductin vitro productionzygote |
| spellingShingle | B. Heidari A. Shirazi M.-M. Naderi M.-M. Akhondi H. Hassanpour A. Sarvari S. Borjian Effect of various co-culture systems on embryo development in ovine Czech Journal of Animal Science mesenchymal stem cell fibroblast oviduct in vitro production zygote |
| title | Effect of various co-culture systems on embryo development in ovine |
| title_full | Effect of various co-culture systems on embryo development in ovine |
| title_fullStr | Effect of various co-culture systems on embryo development in ovine |
| title_full_unstemmed | Effect of various co-culture systems on embryo development in ovine |
| title_short | Effect of various co-culture systems on embryo development in ovine |
| title_sort | effect of various co culture systems on embryo development in ovine |
| topic | mesenchymal stem cell fibroblast oviduct in vitro production zygote |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201310-0002_effect-of-various-co-culture-systems-on-embryo-development-in-ovine.php |
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