Preparation and analysis of polysaccharide from Solanum tuberdsm
The crude Solanum tuberdsm polysaccharides (STP) were extracted with hot water. In the process of extraction, proteins, pigments, small molecules and salts in the mixture were removed by Sevage reagent, diatomite and distilled water dialysis, respectively. In addition, the process conditions of prot...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417723002328 |
| _version_ | 1827876837623070720 |
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| author | Wenjian Yang Gangliang Huang |
| author_facet | Wenjian Yang Gangliang Huang |
| author_sort | Wenjian Yang |
| collection | DOAJ |
| description | The crude Solanum tuberdsm polysaccharides (STP) were extracted with hot water. In the process of extraction, proteins, pigments, small molecules and salts in the mixture were removed by Sevage reagent, diatomite and distilled water dialysis, respectively. In addition, the process conditions of protein removal by response surface methodology (RSM) were optimized, and the optimum process conditions of Sevage method were established as follows: ultrasound power 350 W, ultrasound time 20 min, deproteinization twice, volume ratio of polysaccharide solution to Sevage reagent 1:1 (mL/mL). Under these conditions, the protein removal rate was 93.14%. |
| first_indexed | 2024-03-12T17:26:08Z |
| format | Article |
| id | doaj.art-055862f8f2fa41dd95b76346752247c7 |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-03-12T17:26:08Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-055862f8f2fa41dd95b76346752247c72023-08-05T05:15:33ZengElsevierUltrasonics Sonochemistry1350-41772023-08-0198106520Preparation and analysis of polysaccharide from Solanum tuberdsmWenjian Yang0Gangliang Huang1Key Laboratory of Carbohydrate Science and Engineering, Chongqing Normal University, Chongqing 401331, ChinaCorresponding author.; Key Laboratory of Carbohydrate Science and Engineering, Chongqing Normal University, Chongqing 401331, ChinaThe crude Solanum tuberdsm polysaccharides (STP) were extracted with hot water. In the process of extraction, proteins, pigments, small molecules and salts in the mixture were removed by Sevage reagent, diatomite and distilled water dialysis, respectively. In addition, the process conditions of protein removal by response surface methodology (RSM) were optimized, and the optimum process conditions of Sevage method were established as follows: ultrasound power 350 W, ultrasound time 20 min, deproteinization twice, volume ratio of polysaccharide solution to Sevage reagent 1:1 (mL/mL). Under these conditions, the protein removal rate was 93.14%.http://www.sciencedirect.com/science/article/pii/S1350417723002328Ultrasonic-assisted extractionAnalysisPolysaccharide from Solanum tuberdsm |
| spellingShingle | Wenjian Yang Gangliang Huang Preparation and analysis of polysaccharide from Solanum tuberdsm Ultrasonics Sonochemistry Ultrasonic-assisted extraction Analysis Polysaccharide from Solanum tuberdsm |
| title | Preparation and analysis of polysaccharide from Solanum tuberdsm |
| title_full | Preparation and analysis of polysaccharide from Solanum tuberdsm |
| title_fullStr | Preparation and analysis of polysaccharide from Solanum tuberdsm |
| title_full_unstemmed | Preparation and analysis of polysaccharide from Solanum tuberdsm |
| title_short | Preparation and analysis of polysaccharide from Solanum tuberdsm |
| title_sort | preparation and analysis of polysaccharide from solanum tuberdsm |
| topic | Ultrasonic-assisted extraction Analysis Polysaccharide from Solanum tuberdsm |
| url | http://www.sciencedirect.com/science/article/pii/S1350417723002328 |
| work_keys_str_mv | AT wenjianyang preparationandanalysisofpolysaccharidefromsolanumtuberdsm AT ganglianghuang preparationandanalysisofpolysaccharidefromsolanumtuberdsm |