Electrospun Biodegradable α-Amino Acid-Substituted Poly(organophosphazene) Fiber Mats for Stem Cell Differentiation towards Vascular Smooth Muscle Cells
Mesenchymal stem cells, derived from human-induced pluripotent stem cells (iPSC), are valuable for generating smooth muscle cells (SMCs) for vascular tissue engineering applications. In this study, we synthesized biodegradable α-amino acid-substituted poly(organophosphazene) polymers and electrospun...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/8/1555 |
| _version_ | 1797434265014632448 |
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| author | Meng Wang Shigang Lin Kibret Mequanint |
| author_facet | Meng Wang Shigang Lin Kibret Mequanint |
| author_sort | Meng Wang |
| collection | DOAJ |
| description | Mesenchymal stem cells, derived from human-induced pluripotent stem cells (iPSC), are valuable for generating smooth muscle cells (SMCs) for vascular tissue engineering applications. In this study, we synthesized biodegradable α-amino acid-substituted poly(organophosphazene) polymers and electrospun nano-fibrous scaffolds (~200 nm diameter) to evaluate their suitability as a matrix for differentiation of iPSC-derived mesenchymal stem cells (iMSC) into mature contractile SMCs. Both the polymer synthesis approach and the electrospinning parameters were optimized. Three types of cells, namely iMSC, bone marrow derived mesenchymal stem cells (BM-MSC), and primary human coronary artery SMC, attached and spread on the materials. Although <span style="font-variant: small-caps;">L</span>-ascorbic acid (AA) and transforming growth factor-beta 1 (TGF-β1) were able to differentiate iMSC along the smooth muscle lineage, we showed that the electrospun fibrous mats provided material cues for the enhanced differentiation of iMSCs. Differentiation of iMSC to SMC was characterized by increased transcriptional levels of early to late-stage smooth muscle marker proteins on electrospun fibrous mats. Our findings provide a feasible strategy for engineering functional vascular tissues. |
| first_indexed | 2024-03-09T10:29:44Z |
| format | Article |
| id | doaj.art-055cac6742ab421ea3fb34902e368c4a |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T10:29:44Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-055cac6742ab421ea3fb34902e368c4a2023-12-01T21:20:09ZengMDPI AGPolymers2073-43602022-04-01148155510.3390/polym14081555Electrospun Biodegradable α-Amino Acid-Substituted Poly(organophosphazene) Fiber Mats for Stem Cell Differentiation towards Vascular Smooth Muscle CellsMeng Wang0Shigang Lin1Kibret Mequanint2Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, ON N6A 5B9, CanadaDepartment of Chemical and Biochemical Engineering, The University of Western Ontario, London, ON N6A 5B9, CanadaDepartment of Chemical and Biochemical Engineering, The University of Western Ontario, London, ON N6A 5B9, CanadaMesenchymal stem cells, derived from human-induced pluripotent stem cells (iPSC), are valuable for generating smooth muscle cells (SMCs) for vascular tissue engineering applications. In this study, we synthesized biodegradable α-amino acid-substituted poly(organophosphazene) polymers and electrospun nano-fibrous scaffolds (~200 nm diameter) to evaluate their suitability as a matrix for differentiation of iPSC-derived mesenchymal stem cells (iMSC) into mature contractile SMCs. Both the polymer synthesis approach and the electrospinning parameters were optimized. Three types of cells, namely iMSC, bone marrow derived mesenchymal stem cells (BM-MSC), and primary human coronary artery SMC, attached and spread on the materials. Although <span style="font-variant: small-caps;">L</span>-ascorbic acid (AA) and transforming growth factor-beta 1 (TGF-β1) were able to differentiate iMSC along the smooth muscle lineage, we showed that the electrospun fibrous mats provided material cues for the enhanced differentiation of iMSCs. Differentiation of iMSC to SMC was characterized by increased transcriptional levels of early to late-stage smooth muscle marker proteins on electrospun fibrous mats. Our findings provide a feasible strategy for engineering functional vascular tissues.https://www.mdpi.com/2073-4360/14/8/1555biodegradable poly(organophosphazenes)human mesenchymal stem cellsvascular smooth muscle cellsvascular tissue engineeringelectrospinning |
| spellingShingle | Meng Wang Shigang Lin Kibret Mequanint Electrospun Biodegradable α-Amino Acid-Substituted Poly(organophosphazene) Fiber Mats for Stem Cell Differentiation towards Vascular Smooth Muscle Cells Polymers biodegradable poly(organophosphazenes) human mesenchymal stem cells vascular smooth muscle cells vascular tissue engineering electrospinning |
| title | Electrospun Biodegradable α-Amino Acid-Substituted Poly(organophosphazene) Fiber Mats for Stem Cell Differentiation towards Vascular Smooth Muscle Cells |
| title_full | Electrospun Biodegradable α-Amino Acid-Substituted Poly(organophosphazene) Fiber Mats for Stem Cell Differentiation towards Vascular Smooth Muscle Cells |
| title_fullStr | Electrospun Biodegradable α-Amino Acid-Substituted Poly(organophosphazene) Fiber Mats for Stem Cell Differentiation towards Vascular Smooth Muscle Cells |
| title_full_unstemmed | Electrospun Biodegradable α-Amino Acid-Substituted Poly(organophosphazene) Fiber Mats for Stem Cell Differentiation towards Vascular Smooth Muscle Cells |
| title_short | Electrospun Biodegradable α-Amino Acid-Substituted Poly(organophosphazene) Fiber Mats for Stem Cell Differentiation towards Vascular Smooth Muscle Cells |
| title_sort | electrospun biodegradable α amino acid substituted poly organophosphazene fiber mats for stem cell differentiation towards vascular smooth muscle cells |
| topic | biodegradable poly(organophosphazenes) human mesenchymal stem cells vascular smooth muscle cells vascular tissue engineering electrospinning |
| url | https://www.mdpi.com/2073-4360/14/8/1555 |
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