Effect of DNA extraction in the Rosa canina L. identification under different processing temperature
<p><em>Rosa</em> <em>canina,</em> L. is widely used for medicinal purposes as well as in food industry where it is a valuable source, bioactive compounds and natural colorants. Actually, no specific method is recommended as suitable one for DNA extraction from rose hips...
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| Format: | Article |
| Language: | English |
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HACCP Consulting
2017-01-01
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| Series: | Potravinarstvo |
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| Online Access: | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/717 |
| _version_ | 1818752671775981568 |
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| author | Jana Žiarovská Matúš Kyseľ Radka Cimermanová Ľudmila Knoteková |
| author_facet | Jana Žiarovská Matúš Kyseľ Radka Cimermanová Ľudmila Knoteková |
| author_sort | Jana Žiarovská |
| collection | DOAJ |
| description | <p><em>Rosa</em> <em>canina,</em> L. is widely used for medicinal purposes as well as in food industry where it is a valuable source, bioactive compounds and natural colorants. Actually, no specific method is recommended as suitable one for DNA extraction from rose hips. The aim of the study was to compare three commercial and three non-commercial methods to extract total genomic DNA from rose hips hyphanthium. Four methods are based on the precipitation in principle and two methods are based on resin-binding. Extracted DNA was proved for the effectivity in following PCR. In total, six different DNA isolations was performed for differently heat processes rose hips - fresh hyphanthium, 2-weeks frozen hyphanthium, dried hyphanthium (50 °C) and boiled hyphanthium (100 °C). The amplification parameters of 500 bp chloroplast gene amplicon were evaluated. Obtained amounts of extracted DNA was very variable not only for every individual method used but for individual treatment of samples, too. In general, non-commercial method provided higher amount of extracted DNA, but the A260/280 ratio was lower. When regarding the processing treatment of the samples, high differences were found among the samples untreated by heat and those that were dried or boiled for three of the used extraction methods. All the samples were positive for amplification, but different amounts of amplified product were obtained. The comparison of data for concentrations of extracted DNA and concentrations of amplified product showed large differences when regarding the achieved purity of DNA in extraction.</p> <!--[endif] --> |
| first_indexed | 2024-12-18T04:55:10Z |
| format | Article |
| id | doaj.art-05617c87a46d4d2f979e3ef1b7901b21 |
| institution | Directory Open Access Journal |
| issn | 1337-0960 |
| language | English |
| last_indexed | 2024-12-18T04:55:10Z |
| publishDate | 2017-01-01 |
| publisher | HACCP Consulting |
| record_format | Article |
| series | Potravinarstvo |
| spelling | doaj.art-05617c87a46d4d2f979e3ef1b7901b212022-12-21T21:20:17ZengHACCP ConsultingPotravinarstvo1337-09602017-01-0111119019610.5219/717537Effect of DNA extraction in the Rosa canina L. identification under different processing temperatureJana Žiarovská0Matúš Kyseľ1Radka Cimermanová2Ľudmila Knoteková3Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Department of Genetics and Plant Breeding, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetic and Plant Breeding, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Tr. A. Hlinku 2, 949 76 Nitra Slovakia, undergraduate studentSlovak University of Agriculture, Faculty of Agrobiology and Food Resources, Tr. A. Hlinku 2, 949 76 Nitra Slovakia, undergraduate student<p><em>Rosa</em> <em>canina,</em> L. is widely used for medicinal purposes as well as in food industry where it is a valuable source, bioactive compounds and natural colorants. Actually, no specific method is recommended as suitable one for DNA extraction from rose hips. The aim of the study was to compare three commercial and three non-commercial methods to extract total genomic DNA from rose hips hyphanthium. Four methods are based on the precipitation in principle and two methods are based on resin-binding. Extracted DNA was proved for the effectivity in following PCR. In total, six different DNA isolations was performed for differently heat processes rose hips - fresh hyphanthium, 2-weeks frozen hyphanthium, dried hyphanthium (50 °C) and boiled hyphanthium (100 °C). The amplification parameters of 500 bp chloroplast gene amplicon were evaluated. Obtained amounts of extracted DNA was very variable not only for every individual method used but for individual treatment of samples, too. In general, non-commercial method provided higher amount of extracted DNA, but the A260/280 ratio was lower. When regarding the processing treatment of the samples, high differences were found among the samples untreated by heat and those that were dried or boiled for three of the used extraction methods. All the samples were positive for amplification, but different amounts of amplified product were obtained. The comparison of data for concentrations of extracted DNA and concentrations of amplified product showed large differences when regarding the achieved purity of DNA in extraction.</p> <!--[endif] -->http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/717dog roseDNA extractionPCR effectivity |
| spellingShingle | Jana Žiarovská Matúš Kyseľ Radka Cimermanová Ľudmila Knoteková Effect of DNA extraction in the Rosa canina L. identification under different processing temperature Potravinarstvo dog rose DNA extraction PCR effectivity |
| title | Effect of DNA extraction in the Rosa canina L. identification under different processing temperature |
| title_full | Effect of DNA extraction in the Rosa canina L. identification under different processing temperature |
| title_fullStr | Effect of DNA extraction in the Rosa canina L. identification under different processing temperature |
| title_full_unstemmed | Effect of DNA extraction in the Rosa canina L. identification under different processing temperature |
| title_short | Effect of DNA extraction in the Rosa canina L. identification under different processing temperature |
| title_sort | effect of dna extraction in the rosa canina l identification under different processing temperature |
| topic | dog rose DNA extraction PCR effectivity |
| url | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/717 |
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