Diversity of Culicoides in the middle belt of Ghana with Implications on the transmission of Mansonella perstans; a molecular approach

Abstract Background Culicoides, also known as biting midges, carry pathogens which include Mansonella perstans. Mansonella perstans is a nematode parasite implicated in a number of disease outcomes. Even though a high prevalence of about 75% M. perstans infection has been recorded in some communitie...

Full description

Bibliographic Details
Main Authors: Linda Batsa Debrah, Joseph F. Arthur, Augustine Yeboah, Dorcas O. Owusu, Ernest Adankwah, Isaac Acheampong, Difery Minadzi, Millicent Lamptey, Vera Serwaa Opoku, Wilfred Aniagyei, Monika M. Vivekanandan, Mohammed K. Abass, Amidu Gawusu, Samuel Wanji, Alexander Y. Debrah, Marc Jacobsen, Richard O. Phillips
Format: Article
Language:English
Published: BMC 2024-03-01
Series:Parasites & Vectors
Subjects:
Online Access:https://doi.org/10.1186/s13071-024-06179-8
_version_ 1797259469571227648
author Linda Batsa Debrah
Joseph F. Arthur
Augustine Yeboah
Dorcas O. Owusu
Ernest Adankwah
Isaac Acheampong
Difery Minadzi
Millicent Lamptey
Vera Serwaa Opoku
Wilfred Aniagyei
Monika M. Vivekanandan
Mohammed K. Abass
Amidu Gawusu
Samuel Wanji
Alexander Y. Debrah
Marc Jacobsen
Richard O. Phillips
author_facet Linda Batsa Debrah
Joseph F. Arthur
Augustine Yeboah
Dorcas O. Owusu
Ernest Adankwah
Isaac Acheampong
Difery Minadzi
Millicent Lamptey
Vera Serwaa Opoku
Wilfred Aniagyei
Monika M. Vivekanandan
Mohammed K. Abass
Amidu Gawusu
Samuel Wanji
Alexander Y. Debrah
Marc Jacobsen
Richard O. Phillips
author_sort Linda Batsa Debrah
collection DOAJ
description Abstract Background Culicoides, also known as biting midges, carry pathogens which include Mansonella perstans. Mansonella perstans is a nematode parasite implicated in a number of disease outcomes. Even though a high prevalence of about 75% M. perstans infection has been recorded in some communities in the middle belt of Ghana, and a wide diversity of Culicoides species has been identified, the exact Culicoides species transmitting M. perstans in Ghana has not yet been deciphered. This study therefore aimed at assessing the species diversity of Culicoides and their role in the transmission of M. perstans in the middle belt of Ghana. Methods Culicoides species were sampled from 11 communities in the Asante-Akim North and Sene West districts in the middle belt of Ghana. Centre for Disease Control (CDC) UV light traps, as well as human bait (i.e. human landing catch and engorged catch) methods were used to assess the species abundance and diversity of Culicoides in the study communities in the wet and dry season. A colorimetric Loop-Mediated Isothermal Amplification (LAMP) assay was performed to assess the vector competence of the various Culicoides species. Results A total of 4810 Culicoides from 6 species were sampled. These included Culicoides inornatipennis, C. milnei, C. schultzei, C. grahamii, C. neavei, and C. imicola. Culicoides imicola was the most abundant species (56%) followed by C. grahamii (16%). Light traps sampled the most diverse species (6 species). Human landing catch and engorged catch methods identified three anthropophilic species, C. grahamii, C. milnei, and C. inornatipennis, with C. grahamii being the most anthropophilic with a peak biting time between the hours of 5 p.m. to 6 p.m. Generally, there was relatively higher species abundance in the wet than dry season. LAMP assay identified C. grahamii as the potential vector for M. perstans transmission in the middle belt of Ghana. Conclusions For the first time, we have demonstrated that C. grahamii is the potential competent vector for M. perstans transmission in the middle belt of Ghana. It is more abundant in the rainy season and has a peak biting time between the hours of 5 and 6 p.m. Graphical Abstract
first_indexed 2024-04-24T23:09:56Z
format Article
id doaj.art-0565446514464f3bbb76626d99ec9524
institution Directory Open Access Journal
issn 1756-3305
language English
last_indexed 2024-04-24T23:09:56Z
publishDate 2024-03-01
publisher BMC
record_format Article
series Parasites & Vectors
spelling doaj.art-0565446514464f3bbb76626d99ec95242024-03-17T12:17:02ZengBMCParasites & Vectors1756-33052024-03-011711910.1186/s13071-024-06179-8Diversity of Culicoides in the middle belt of Ghana with Implications on the transmission of Mansonella perstans; a molecular approachLinda Batsa Debrah0Joseph F. Arthur1Augustine Yeboah2Dorcas O. Owusu3Ernest Adankwah4Isaac Acheampong5Difery Minadzi6Millicent Lamptey7Vera Serwaa Opoku8Wilfred Aniagyei9Monika M. Vivekanandan10Mohammed K. Abass11Amidu Gawusu12Samuel Wanji13Alexander Y. Debrah14Marc Jacobsen15Richard O. Phillips16Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Agogo Presbyterian HospitalSene West Health DirectorateDepartment of Microbiology and Parasitology, University of BueaKumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, University Hospital Duesseldorf, Heinrich-Heine UniversityKumasi Centre for Collaborative Research in Tropical Medicine (KCCR)Abstract Background Culicoides, also known as biting midges, carry pathogens which include Mansonella perstans. Mansonella perstans is a nematode parasite implicated in a number of disease outcomes. Even though a high prevalence of about 75% M. perstans infection has been recorded in some communities in the middle belt of Ghana, and a wide diversity of Culicoides species has been identified, the exact Culicoides species transmitting M. perstans in Ghana has not yet been deciphered. This study therefore aimed at assessing the species diversity of Culicoides and their role in the transmission of M. perstans in the middle belt of Ghana. Methods Culicoides species were sampled from 11 communities in the Asante-Akim North and Sene West districts in the middle belt of Ghana. Centre for Disease Control (CDC) UV light traps, as well as human bait (i.e. human landing catch and engorged catch) methods were used to assess the species abundance and diversity of Culicoides in the study communities in the wet and dry season. A colorimetric Loop-Mediated Isothermal Amplification (LAMP) assay was performed to assess the vector competence of the various Culicoides species. Results A total of 4810 Culicoides from 6 species were sampled. These included Culicoides inornatipennis, C. milnei, C. schultzei, C. grahamii, C. neavei, and C. imicola. Culicoides imicola was the most abundant species (56%) followed by C. grahamii (16%). Light traps sampled the most diverse species (6 species). Human landing catch and engorged catch methods identified three anthropophilic species, C. grahamii, C. milnei, and C. inornatipennis, with C. grahamii being the most anthropophilic with a peak biting time between the hours of 5 p.m. to 6 p.m. Generally, there was relatively higher species abundance in the wet than dry season. LAMP assay identified C. grahamii as the potential vector for M. perstans transmission in the middle belt of Ghana. Conclusions For the first time, we have demonstrated that C. grahamii is the potential competent vector for M. perstans transmission in the middle belt of Ghana. It is more abundant in the rainy season and has a peak biting time between the hours of 5 and 6 p.m. Graphical Abstracthttps://doi.org/10.1186/s13071-024-06179-8Culicoides speciesCulicoides grahamiiMansonella perstansMicrofilariae
spellingShingle Linda Batsa Debrah
Joseph F. Arthur
Augustine Yeboah
Dorcas O. Owusu
Ernest Adankwah
Isaac Acheampong
Difery Minadzi
Millicent Lamptey
Vera Serwaa Opoku
Wilfred Aniagyei
Monika M. Vivekanandan
Mohammed K. Abass
Amidu Gawusu
Samuel Wanji
Alexander Y. Debrah
Marc Jacobsen
Richard O. Phillips
Diversity of Culicoides in the middle belt of Ghana with Implications on the transmission of Mansonella perstans; a molecular approach
Parasites & Vectors
Culicoides species
Culicoides grahamii
Mansonella perstans
Microfilariae
title Diversity of Culicoides in the middle belt of Ghana with Implications on the transmission of Mansonella perstans; a molecular approach
title_full Diversity of Culicoides in the middle belt of Ghana with Implications on the transmission of Mansonella perstans; a molecular approach
title_fullStr Diversity of Culicoides in the middle belt of Ghana with Implications on the transmission of Mansonella perstans; a molecular approach
title_full_unstemmed Diversity of Culicoides in the middle belt of Ghana with Implications on the transmission of Mansonella perstans; a molecular approach
title_short Diversity of Culicoides in the middle belt of Ghana with Implications on the transmission of Mansonella perstans; a molecular approach
title_sort diversity of culicoides in the middle belt of ghana with implications on the transmission of mansonella perstans a molecular approach
topic Culicoides species
Culicoides grahamii
Mansonella perstans
Microfilariae
url https://doi.org/10.1186/s13071-024-06179-8
work_keys_str_mv AT lindabatsadebrah diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT josephfarthur diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT augustineyeboah diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT dorcasoowusu diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT ernestadankwah diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT isaacacheampong diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT diferyminadzi diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT millicentlamptey diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT veraserwaaopoku diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT wilfredaniagyei diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT monikamvivekanandan diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT mohammedkabass diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT amidugawusu diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT samuelwanji diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT alexanderydebrah diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT marcjacobsen diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach
AT richardophillips diversityofculicoidesinthemiddlebeltofghanawithimplicationsonthetransmissionofmansonellaperstansamolecularapproach