Alloreactivity and Immunosuppressive Properties of Articular Chondrocytes from Osteoarthritic Cartilage
Purpose To determine whether articular chondrocytes derived from osteoarthritic knee joints could evoke alloreactive proliferation of peripheral blood mononuclear cells (PBMC) and inhibit mitogenic activity of polyclonally activated CD4 + major histocompatibility complex (MHC) class II–restricted T...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2016-08-01
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| Series: | Journal of Orthopaedic Surgery |
| Online Access: | https://doi.org/10.1177/1602400222 |
| _version_ | 1819004241613684736 |
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| author | Satomi Abe Hitoshi Nochi Hiroshi Ito |
| author_facet | Satomi Abe Hitoshi Nochi Hiroshi Ito |
| author_sort | Satomi Abe |
| collection | DOAJ |
| description | Purpose To determine whether articular chondrocytes derived from osteoarthritic knee joints could evoke alloreactive proliferation of peripheral blood mononuclear cells (PBMC) and inhibit mitogenic activity of polyclonally activated CD4 + major histocompatibility complex (MHC) class II–restricted T cells in vitro . Methods Osteoarthritic cartilages of 17 patients aged 61 to 85 years were harvested during total knee arthroplasty. Chondrocytes were cultured for experiments. PBMCs, CD4 + T cells, CD8 + T cells, and CD14 + monocytes from healthy subjects were also used. To investigate the allogeneic response and immunosuppressive properties of chondrocytes, assays for one-way mixed lymphocyte reaction (MLR), apoptosis, activated CD4 + T-cell proliferation, and cytotoxic CD8 + T-cells were performed. Chondrocyte cell-surface antigens were examined using flow cytometry. Results Chondrocytes failed to trigger an allogeneic PBMC reaction and did not induce apoptosis of allogeneic PBMCs in the MLR assay. Chondrocytes inhibited the proliferation of polyclonally activated CD4 + T cells via cell-cell contact and escaped the allogeneic cytotoxic reactivity of CD8 + T cells. Chondrocytes expressed MHC class I but not MHC class II molecules or B7-1/-2-positive co-stimulatory molecules. Conclusion Chondrocytes from osteoarthritic knees in older patients exhibited similar immunomodulatory properties in vitro to those in juveniles or adults. |
| first_indexed | 2024-12-20T23:33:46Z |
| format | Article |
| id | doaj.art-05694eea8fab45cead92c4f351d3fa49 |
| institution | Directory Open Access Journal |
| issn | 2309-4990 |
| language | English |
| last_indexed | 2024-12-20T23:33:46Z |
| publishDate | 2016-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Orthopaedic Surgery |
| spelling | doaj.art-05694eea8fab45cead92c4f351d3fa492022-12-21T19:23:15ZengSAGE PublishingJournal of Orthopaedic Surgery2309-49902016-08-012410.1177/1602400222Alloreactivity and Immunosuppressive Properties of Articular Chondrocytes from Osteoarthritic CartilageSatomi AbeHitoshi NochiHiroshi ItoPurpose To determine whether articular chondrocytes derived from osteoarthritic knee joints could evoke alloreactive proliferation of peripheral blood mononuclear cells (PBMC) and inhibit mitogenic activity of polyclonally activated CD4 + major histocompatibility complex (MHC) class II–restricted T cells in vitro . Methods Osteoarthritic cartilages of 17 patients aged 61 to 85 years were harvested during total knee arthroplasty. Chondrocytes were cultured for experiments. PBMCs, CD4 + T cells, CD8 + T cells, and CD14 + monocytes from healthy subjects were also used. To investigate the allogeneic response and immunosuppressive properties of chondrocytes, assays for one-way mixed lymphocyte reaction (MLR), apoptosis, activated CD4 + T-cell proliferation, and cytotoxic CD8 + T-cells were performed. Chondrocyte cell-surface antigens were examined using flow cytometry. Results Chondrocytes failed to trigger an allogeneic PBMC reaction and did not induce apoptosis of allogeneic PBMCs in the MLR assay. Chondrocytes inhibited the proliferation of polyclonally activated CD4 + T cells via cell-cell contact and escaped the allogeneic cytotoxic reactivity of CD8 + T cells. Chondrocytes expressed MHC class I but not MHC class II molecules or B7-1/-2-positive co-stimulatory molecules. Conclusion Chondrocytes from osteoarthritic knees in older patients exhibited similar immunomodulatory properties in vitro to those in juveniles or adults.https://doi.org/10.1177/1602400222 |
| spellingShingle | Satomi Abe Hitoshi Nochi Hiroshi Ito Alloreactivity and Immunosuppressive Properties of Articular Chondrocytes from Osteoarthritic Cartilage Journal of Orthopaedic Surgery |
| title | Alloreactivity and Immunosuppressive Properties of Articular Chondrocytes from Osteoarthritic Cartilage |
| title_full | Alloreactivity and Immunosuppressive Properties of Articular Chondrocytes from Osteoarthritic Cartilage |
| title_fullStr | Alloreactivity and Immunosuppressive Properties of Articular Chondrocytes from Osteoarthritic Cartilage |
| title_full_unstemmed | Alloreactivity and Immunosuppressive Properties of Articular Chondrocytes from Osteoarthritic Cartilage |
| title_short | Alloreactivity and Immunosuppressive Properties of Articular Chondrocytes from Osteoarthritic Cartilage |
| title_sort | alloreactivity and immunosuppressive properties of articular chondrocytes from osteoarthritic cartilage |
| url | https://doi.org/10.1177/1602400222 |
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