Recent Advances in the Physicochemical Properties and Biotechnological Application of <i>Vitreoscilla</i> Hemoglobin
<i>Vitreoscilla</i> hemoglobin (VHb), the first discovered bacterial hemoglobin, is a soluble heme-binding protein with a faster rate of oxygen dissociation. Since it can enhance cell growth, product synthesis and stress tolerance, VHb has been widely applied in the field of metabolic en...
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MDPI AG
2021-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/7/1455 |
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| author | Fei Yu Xinrui Zhao Ziwei Wang Luyao Liu Lingfeng Yi Jingwen Zhou Jianghua Li Jian Chen Guocheng Du |
| author_facet | Fei Yu Xinrui Zhao Ziwei Wang Luyao Liu Lingfeng Yi Jingwen Zhou Jianghua Li Jian Chen Guocheng Du |
| author_sort | Fei Yu |
| collection | DOAJ |
| description | <i>Vitreoscilla</i> hemoglobin (VHb), the first discovered bacterial hemoglobin, is a soluble heme-binding protein with a faster rate of oxygen dissociation. Since it can enhance cell growth, product synthesis and stress tolerance, VHb has been widely applied in the field of metabolic engineering for microorganisms, plants, and animals. Especially under oxygen-limited conditions, VHb can interact with terminal oxidase to deliver enough oxygen to achieve high-cell-density fermentation. In recent years, with the development of bioinformatics and synthetic biology, several novel physicochemical properties and metabolic regulatory effects of VHb have been discovered and numerous strategies have been utilized to enhance the expression level of VHb in various hosts, which greatly promotes its applications in biotechnology. Thus, in this review, the new information regarding structure, function and expressional tactics for VHb is summarized to understand its latest applications and pave a new way for the future improvement of biosynthesis for other products. |
| first_indexed | 2024-03-10T09:31:05Z |
| format | Article |
| id | doaj.art-0569f011e1c645d1b8f44d832036974f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T09:31:05Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-0569f011e1c645d1b8f44d832036974f2023-11-22T04:25:58ZengMDPI AGMicroorganisms2076-26072021-07-0197145510.3390/microorganisms9071455Recent Advances in the Physicochemical Properties and Biotechnological Application of <i>Vitreoscilla</i> HemoglobinFei Yu0Xinrui Zhao1Ziwei Wang2Luyao Liu3Lingfeng Yi4Jingwen Zhou5Jianghua Li6Jian Chen7Guocheng Du8Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, ChinaKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China<i>Vitreoscilla</i> hemoglobin (VHb), the first discovered bacterial hemoglobin, is a soluble heme-binding protein with a faster rate of oxygen dissociation. Since it can enhance cell growth, product synthesis and stress tolerance, VHb has been widely applied in the field of metabolic engineering for microorganisms, plants, and animals. Especially under oxygen-limited conditions, VHb can interact with terminal oxidase to deliver enough oxygen to achieve high-cell-density fermentation. In recent years, with the development of bioinformatics and synthetic biology, several novel physicochemical properties and metabolic regulatory effects of VHb have been discovered and numerous strategies have been utilized to enhance the expression level of VHb in various hosts, which greatly promotes its applications in biotechnology. Thus, in this review, the new information regarding structure, function and expressional tactics for VHb is summarized to understand its latest applications and pave a new way for the future improvement of biosynthesis for other products.https://www.mdpi.com/2076-2607/9/7/1455<i>Vitreoscilla</i> hemoglobinhigh-cell-density fermentationphysicochemical propertiesmetabolic regulationexpressional tacticsapplications |
| spellingShingle | Fei Yu Xinrui Zhao Ziwei Wang Luyao Liu Lingfeng Yi Jingwen Zhou Jianghua Li Jian Chen Guocheng Du Recent Advances in the Physicochemical Properties and Biotechnological Application of <i>Vitreoscilla</i> Hemoglobin Microorganisms <i>Vitreoscilla</i> hemoglobin high-cell-density fermentation physicochemical properties metabolic regulation expressional tactics applications |
| title | Recent Advances in the Physicochemical Properties and Biotechnological Application of <i>Vitreoscilla</i> Hemoglobin |
| title_full | Recent Advances in the Physicochemical Properties and Biotechnological Application of <i>Vitreoscilla</i> Hemoglobin |
| title_fullStr | Recent Advances in the Physicochemical Properties and Biotechnological Application of <i>Vitreoscilla</i> Hemoglobin |
| title_full_unstemmed | Recent Advances in the Physicochemical Properties and Biotechnological Application of <i>Vitreoscilla</i> Hemoglobin |
| title_short | Recent Advances in the Physicochemical Properties and Biotechnological Application of <i>Vitreoscilla</i> Hemoglobin |
| title_sort | recent advances in the physicochemical properties and biotechnological application of i vitreoscilla i hemoglobin |
| topic | <i>Vitreoscilla</i> hemoglobin high-cell-density fermentation physicochemical properties metabolic regulation expressional tactics applications |
| url | https://www.mdpi.com/2076-2607/9/7/1455 |
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