Dsg2 ectodomain organization increases throughout desmosome assembly
ABSTRACTDesmosomes are intercellular junctions that regulate mechanical integrity in epithelia and cardiac muscle. Dynamic desmosome remodeling is essential for wound healing and development, yet the mechanisms governing junction assembly remain elusive. While we and others have shown that cadherin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
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| Series: | Cell Adhesion & Migration |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19336918.2024.2333366 |
| _version_ | 1797226668078661632 |
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| author | William F. Dean Rose M. Albert Tomasz J. Nawara Melanie Ubil Reena R. Beggs Alexa L. Mattheyses |
| author_facet | William F. Dean Rose M. Albert Tomasz J. Nawara Melanie Ubil Reena R. Beggs Alexa L. Mattheyses |
| author_sort | William F. Dean |
| collection | DOAJ |
| description | ABSTRACTDesmosomes are intercellular junctions that regulate mechanical integrity in epithelia and cardiac muscle. Dynamic desmosome remodeling is essential for wound healing and development, yet the mechanisms governing junction assembly remain elusive. While we and others have shown that cadherin ectodomains are highly organized, how this ordered architecture emerges during assembly is unknown. Using fluorescence polarization microscopy, we show that desmoglein 2 (Dsg2) ectodomain order gradually increases during 8 h of assembly, coinciding with increasing adhesive strength. In a scratch wound assay, we observed a similar increase in order in desmosomes assembling at the leading edge of migratory cells. Together, our findings indicate that cadherin organization is a hallmark of desmosome maturity and may play a role in conferring adhesive strength. |
| first_indexed | 2024-04-24T14:28:34Z |
| format | Article |
| id | doaj.art-056d2e93e57e49f7bbfe3e8c0aa65168 |
| institution | Directory Open Access Journal |
| issn | 1933-6918 1933-6926 |
| language | English |
| last_indexed | 2024-04-24T14:28:34Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cell Adhesion & Migration |
| spelling | doaj.art-056d2e93e57e49f7bbfe3e8c0aa651682024-04-03T04:03:47ZengTaylor & Francis GroupCell Adhesion & Migration1933-69181933-69262024-12-0118111310.1080/19336918.2024.2333366Dsg2 ectodomain organization increases throughout desmosome assemblyWilliam F. Dean0Rose M. Albert1Tomasz J. Nawara2Melanie Ubil3Reena R. Beggs4Alexa L. Mattheyses5Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USADepartment of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USADepartment of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USADepartment of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USADepartment of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USADepartment of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USAABSTRACTDesmosomes are intercellular junctions that regulate mechanical integrity in epithelia and cardiac muscle. Dynamic desmosome remodeling is essential for wound healing and development, yet the mechanisms governing junction assembly remain elusive. While we and others have shown that cadherin ectodomains are highly organized, how this ordered architecture emerges during assembly is unknown. Using fluorescence polarization microscopy, we show that desmoglein 2 (Dsg2) ectodomain order gradually increases during 8 h of assembly, coinciding with increasing adhesive strength. In a scratch wound assay, we observed a similar increase in order in desmosomes assembling at the leading edge of migratory cells. Together, our findings indicate that cadherin organization is a hallmark of desmosome maturity and may play a role in conferring adhesive strength.https://www.tandfonline.com/doi/10.1080/19336918.2024.2333366Assemblycadherincell-cell adhesiondesmosomefluorescence polarization |
| spellingShingle | William F. Dean Rose M. Albert Tomasz J. Nawara Melanie Ubil Reena R. Beggs Alexa L. Mattheyses Dsg2 ectodomain organization increases throughout desmosome assembly Cell Adhesion & Migration Assembly cadherin cell-cell adhesion desmosome fluorescence polarization |
| title | Dsg2 ectodomain organization increases throughout desmosome assembly |
| title_full | Dsg2 ectodomain organization increases throughout desmosome assembly |
| title_fullStr | Dsg2 ectodomain organization increases throughout desmosome assembly |
| title_full_unstemmed | Dsg2 ectodomain organization increases throughout desmosome assembly |
| title_short | Dsg2 ectodomain organization increases throughout desmosome assembly |
| title_sort | dsg2 ectodomain organization increases throughout desmosome assembly |
| topic | Assembly cadherin cell-cell adhesion desmosome fluorescence polarization |
| url | https://www.tandfonline.com/doi/10.1080/19336918.2024.2333366 |
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