Flow Cytometry Detection of Bacterial Cell Entrapment within the Chitosan Hydrogel and Antibacterial Property of Extracted Chitosan
<div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><strong><em>Background</em></strong><strong>:<em> </em></strong> ...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2016-09-01
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| Series: | Journal of Medical Bacteriology |
| Subjects: | |
| Online Access: | http://jmb.tums.ac.ir/index.php/jmb/article/view/188 |
| _version_ | 1811205615782985728 |
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| author | Nafise Sadat Majidi Giti Emtiazi Samaneh Shahrokh Esfahani |
| author_facet | Nafise Sadat Majidi Giti Emtiazi Samaneh Shahrokh Esfahani |
| author_sort | Nafise Sadat Majidi |
| collection | DOAJ |
| description | <div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><strong><em>Background</em></strong><strong>:<em> </em></strong> Chitosan is unbranched polysaccharide composed of D-glucosamine and N-acetyl-D-glucosamine. Chitosan, derived from shrimp shell, has broad antimicrobial properties against Gram-negative, Gram-positive bacteria and fungi.</p><p> </p><p><strong><em>Methods</em></strong><strong>:<em> </em></strong> Chitosan was extracted from shrimp shell and studied for cell entrapment and anti-bacterial properties. The hydrogel chitosan was used as the beads for cell entrapment and chitosan beads were designed to deliver cells and nutrients. These data confirmed with flow cytometric analyses. </p><p> </p><p><strong><em>Results</em></strong><strong>:<em> </em></strong><em> </em> Experimental results exhibited that internal diffusion through the chitosan matrix was the main mechanism for whole gelation by TPP (Tri-polyphosphate). The minimum inhibitory concentration (MIC) for chitosan against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> was 16 and 32 μg/ml respectively.</p><p> </p></td></tr></tbody></table></div><strong><em>Conclusion</em>:<em> </em></strong> Despite the antimicrobial properties of chitosan, trapped bacteria in the gel network were alive and were chelated indicating that their access to the outside was limited. |
| first_indexed | 2024-04-12T03:34:02Z |
| format | Article |
| id | doaj.art-056ef218fe3d43a4af280bd5f699f09f |
| institution | Directory Open Access Journal |
| issn | 2251-8649 2322-2581 |
| language | English |
| last_indexed | 2024-04-12T03:34:02Z |
| publishDate | 2016-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Bacteriology |
| spelling | doaj.art-056ef218fe3d43a4af280bd5f699f09f2022-12-22T03:49:28ZengTehran University of Medical SciencesJournal of Medical Bacteriology2251-86492322-25812016-09-0153-4914191Flow Cytometry Detection of Bacterial Cell Entrapment within the Chitosan Hydrogel and Antibacterial Property of Extracted ChitosanNafise Sadat Majidi0Giti Emtiazi1Samaneh Shahrokh Esfahani2Biology Department, University of Isfahan, Isfahan, Iran.Biology Department, University of Isfahan, Isfahan, Iran.Biology Department, University of Isfahan, Isfahan, Iran.<div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><strong><em>Background</em></strong><strong>:<em> </em></strong> Chitosan is unbranched polysaccharide composed of D-glucosamine and N-acetyl-D-glucosamine. Chitosan, derived from shrimp shell, has broad antimicrobial properties against Gram-negative, Gram-positive bacteria and fungi.</p><p> </p><p><strong><em>Methods</em></strong><strong>:<em> </em></strong> Chitosan was extracted from shrimp shell and studied for cell entrapment and anti-bacterial properties. The hydrogel chitosan was used as the beads for cell entrapment and chitosan beads were designed to deliver cells and nutrients. These data confirmed with flow cytometric analyses. </p><p> </p><p><strong><em>Results</em></strong><strong>:<em> </em></strong><em> </em> Experimental results exhibited that internal diffusion through the chitosan matrix was the main mechanism for whole gelation by TPP (Tri-polyphosphate). The minimum inhibitory concentration (MIC) for chitosan against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> was 16 and 32 μg/ml respectively.</p><p> </p></td></tr></tbody></table></div><strong><em>Conclusion</em>:<em> </em></strong> Despite the antimicrobial properties of chitosan, trapped bacteria in the gel network were alive and were chelated indicating that their access to the outside was limited.http://jmb.tums.ac.ir/index.php/jmb/article/view/188ChitosanChitinTPPAntibacterial activityFlow cytometry |
| spellingShingle | Nafise Sadat Majidi Giti Emtiazi Samaneh Shahrokh Esfahani Flow Cytometry Detection of Bacterial Cell Entrapment within the Chitosan Hydrogel and Antibacterial Property of Extracted Chitosan Journal of Medical Bacteriology Chitosan Chitin TPP Antibacterial activity Flow cytometry |
| title | Flow Cytometry Detection of Bacterial Cell Entrapment within the Chitosan Hydrogel and Antibacterial Property of Extracted Chitosan |
| title_full | Flow Cytometry Detection of Bacterial Cell Entrapment within the Chitosan Hydrogel and Antibacterial Property of Extracted Chitosan |
| title_fullStr | Flow Cytometry Detection of Bacterial Cell Entrapment within the Chitosan Hydrogel and Antibacterial Property of Extracted Chitosan |
| title_full_unstemmed | Flow Cytometry Detection of Bacterial Cell Entrapment within the Chitosan Hydrogel and Antibacterial Property of Extracted Chitosan |
| title_short | Flow Cytometry Detection of Bacterial Cell Entrapment within the Chitosan Hydrogel and Antibacterial Property of Extracted Chitosan |
| title_sort | flow cytometry detection of bacterial cell entrapment within the chitosan hydrogel and antibacterial property of extracted chitosan |
| topic | Chitosan Chitin TPP Antibacterial activity Flow cytometry |
| url | http://jmb.tums.ac.ir/index.php/jmb/article/view/188 |
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