DIAGNOSTIC POTENTIAL OF THE ELECTRON MICROSCOPIC ANALYSIS OF HUMAN LACRIMAL FLUID
Purpose. To evaluate the possibility of application of electron microscopic analysis of human lacrimal fluid (LF) in the development of methods for early diagnosis and prognosis of ophthalmic diseases.Material and methods. Specimens of the reflex LF were collected from the lower conjunctival arch of...
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| Format: | Article |
| Language: | Russian |
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Publishing house "Ophthalmology"
2015-12-01
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| Series: | Офтальмохирургия |
| Subjects: | |
| Online Access: | https://www.ophthalmosurgery.ru/jour/article/view/175 |
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| author | A. E. Grigoryeva A. V. Eremina I. B. Druzhinin D. V. Chernykh E. V. Varvarinsky E. I. Ryabchikova |
| author_facet | A. E. Grigoryeva A. V. Eremina I. B. Druzhinin D. V. Chernykh E. V. Varvarinsky E. I. Ryabchikova |
| author_sort | A. E. Grigoryeva |
| collection | DOAJ |
| description | Purpose. To evaluate the possibility of application of electron microscopic analysis of human lacrimal fluid (LF) in the development of methods for early diagnosis and prognosis of ophthalmic diseases.Material and methods. Specimens of the reflex LF were collected from the lower conjunctival arch of eyes in 20 patients with a diagnosis of diabetic retinopathy, hyperopia, glaucoma, cataract and macular degeneration, and pelleted by centrifugation. The liquid portion of LF was examined by the negative staining method, and the pellets were processed by the ultrathin sectioning method, and the sections were studied in the electron microscope JEM-1400 (JEOL, Japan).Results. The liquid portion of LF contained macromolecular aggregates, microparticles and exosomes, the number and morphology of which varied depending on the eye disease. Ultrathin sections of the LF pellets showed fibrous matrix, epithelial cells, lymphocytes and polymorphonuclear leukocytes, cell debris, and small roundish vesicles (150- 300 nm) on the surface bearing the protein «filaments» penetrating inside. The cellular composition of LF pellets varied depending on the disease of eye.Conclusions. Identification of the obvious differences in the content and morphology of the structural components of the LF indicates a high diagnostic potential of the LF. Electron microscopy of LF looks promising for a search of ultrastructural diagnostic and prognostic markers of ophthalmic diseases. |
| first_indexed | 2024-04-10T21:14:52Z |
| format | Article |
| id | doaj.art-057173e866114c79b011dbeccb4edcc0 |
| institution | Directory Open Access Journal |
| issn | 0235-4160 2312-4970 |
| language | Russian |
| last_indexed | 2024-04-10T21:14:52Z |
| publishDate | 2015-12-01 |
| publisher | Publishing house "Ophthalmology" |
| record_format | Article |
| series | Офтальмохирургия |
| spelling | doaj.art-057173e866114c79b011dbeccb4edcc02023-01-20T12:34:50ZrusPublishing house "Ophthalmology"Офтальмохирургия0235-41602312-49702015-12-0104104107173DIAGNOSTIC POTENTIAL OF THE ELECTRON MICROSCOPIC ANALYSIS OF HUMAN LACRIMAL FLUIDA. E. Grigoryeva0A. V. Eremina1I. B. Druzhinin2D. V. Chernykh3E. V. Varvarinsky4E. I. Ryabchikova5The Institute of Chemical Biology and Fundamental Medicine of the Russian Academy of Sciences, NovosibirskThe Novosibirsk Branch of the S. Fyodorov Eye Microsurgery Federal State Institution, NovosibirskThe Novosibirsk Branch of the S. Fyodorov Eye Microsurgery Federal State Institution, NovosibirskThe Novosibirsk Branch of the S. Fyodorov Eye Microsurgery Federal State Institution, NovosibirskThe Novosibirsk Branch of the S. Fyodorov Eye Microsurgery Federal State Institution, NovosibirskThe Institute of Chemical Biology and Fundamental Medicine of the Russian Academy of Sciences, NovosibirskPurpose. To evaluate the possibility of application of electron microscopic analysis of human lacrimal fluid (LF) in the development of methods for early diagnosis and prognosis of ophthalmic diseases.Material and methods. Specimens of the reflex LF were collected from the lower conjunctival arch of eyes in 20 patients with a diagnosis of diabetic retinopathy, hyperopia, glaucoma, cataract and macular degeneration, and pelleted by centrifugation. The liquid portion of LF was examined by the negative staining method, and the pellets were processed by the ultrathin sectioning method, and the sections were studied in the electron microscope JEM-1400 (JEOL, Japan).Results. The liquid portion of LF contained macromolecular aggregates, microparticles and exosomes, the number and morphology of which varied depending on the eye disease. Ultrathin sections of the LF pellets showed fibrous matrix, epithelial cells, lymphocytes and polymorphonuclear leukocytes, cell debris, and small roundish vesicles (150- 300 nm) on the surface bearing the protein «filaments» penetrating inside. The cellular composition of LF pellets varied depending on the disease of eye.Conclusions. Identification of the obvious differences in the content and morphology of the structural components of the LF indicates a high diagnostic potential of the LF. Electron microscopy of LF looks promising for a search of ultrastructural diagnostic and prognostic markers of ophthalmic diseases.https://www.ophthalmosurgery.ru/jour/article/view/175lacrimal fluidelectron microscopymicroparticlesexosomesophthalmologic diseases |
| spellingShingle | A. E. Grigoryeva A. V. Eremina I. B. Druzhinin D. V. Chernykh E. V. Varvarinsky E. I. Ryabchikova DIAGNOSTIC POTENTIAL OF THE ELECTRON MICROSCOPIC ANALYSIS OF HUMAN LACRIMAL FLUID Офтальмохирургия lacrimal fluid electron microscopy microparticles exosomes ophthalmologic diseases |
| title | DIAGNOSTIC POTENTIAL OF THE ELECTRON MICROSCOPIC ANALYSIS OF HUMAN LACRIMAL FLUID |
| title_full | DIAGNOSTIC POTENTIAL OF THE ELECTRON MICROSCOPIC ANALYSIS OF HUMAN LACRIMAL FLUID |
| title_fullStr | DIAGNOSTIC POTENTIAL OF THE ELECTRON MICROSCOPIC ANALYSIS OF HUMAN LACRIMAL FLUID |
| title_full_unstemmed | DIAGNOSTIC POTENTIAL OF THE ELECTRON MICROSCOPIC ANALYSIS OF HUMAN LACRIMAL FLUID |
| title_short | DIAGNOSTIC POTENTIAL OF THE ELECTRON MICROSCOPIC ANALYSIS OF HUMAN LACRIMAL FLUID |
| title_sort | diagnostic potential of the electron microscopic analysis of human lacrimal fluid |
| topic | lacrimal fluid electron microscopy microparticles exosomes ophthalmologic diseases |
| url | https://www.ophthalmosurgery.ru/jour/article/view/175 |
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