Pro-Neurotensin as a Potential Novel Diagnostic Biomarker for Detection of Nonalcoholic Fatty Liver Disease
Amal A Mohamed,1 Dina M Abo-Elmatty,2 Omnia Ezzat,3 Noha M Mesbah,2 Nada S Ali,3 Aliaa Sayed Abd El Fatah,4 Eman Alsayed,5 Mahmoud Hamada,6 Alshymaa A Hassnine,7 Sherief Abd-Elsalam,8 Ahmed Abdelghani,9 Mohamed Badr Hassan,9 Shaimaa A Fattah2 1Biochemistry and Molecular Biology Department, National...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2022-06-01
|
| Series: | Diabetes, Metabolic Syndrome and Obesity |
| Subjects: | |
| Online Access: | https://www.dovepress.com/pro-neurotensin-as-a-potential-novel-diagnostic-biomarker-for-detectio-peer-reviewed-fulltext-article-DMSO |
| _version_ | 1797704367263973376 |
|---|---|
| author | Mohamed AA Abo-Elmatty DM Ezzat O Mesbah NM Ali NS Abd El Fatah AS Alsayed E Hamada M Hassnine AA Abd-Elsalam S Abdelghani A Hassan MB Fattah SA |
| author_facet | Mohamed AA Abo-Elmatty DM Ezzat O Mesbah NM Ali NS Abd El Fatah AS Alsayed E Hamada M Hassnine AA Abd-Elsalam S Abdelghani A Hassan MB Fattah SA |
| author_sort | Mohamed AA |
| collection | DOAJ |
| description | Amal A Mohamed,1 Dina M Abo-Elmatty,2 Omnia Ezzat,3 Noha M Mesbah,2 Nada S Ali,3 Aliaa Sayed Abd El Fatah,4 Eman Alsayed,5 Mahmoud Hamada,6 Alshymaa A Hassnine,7 Sherief Abd-Elsalam,8 Ahmed Abdelghani,9 Mohamed Badr Hassan,9 Shaimaa A Fattah2 1Biochemistry and Molecular Biology Department, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt; 2Biochemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt; 3Biochemistry Department, Faculty of Pharmacy, Egyptian Russian University, Cairo, Egypt; 4Internal Medicine Department, Faculty of Medicine, Minia University, Minia, Egypt; 5Department of Clinical Pathology, Minia University Hospital, Minia, Egypt; 6Internal Medicine Department, Faculty of Medicine, Benha University, Benha, Egypt; 7Department of Gastroenterology and Tropical Medicine, Faculty of Medicine, Minia University, Minia, Egypt; 8Tropical Medicine Department, Tanta University, Tanta, Egypt; 9Internal Medicine Department, Faculty of Medicine, Cairo University, Cairo, EgyptCorrespondence: Sherief Abd-Elsalam, Department of Tropical Medicine, Faculty of Medicine, Tanta University, El-Bahr Street, Tanta, Egypt, Tel +201147773440, Email sherif.abdelbaky@med.tanta.edu.egBackground and Aims: Currently, liver biopsy is the gold standard method for diagnosis of non-alcoholic fatty liver severity. It is critical to develop non-invasive diagnostic method to diagnose nonalcoholic fatty liver rather than invasive techniques. Our case–control study was to address the value of circulating miRNA-122 and serum pro-neurotensin as a potential non-invasive biomarker for the diagnosis of non-alcoholic fatty acid diseases.Methods: Clinical assessment, laboratory investigations, and anthropometric measurements were reported for 157 patients with proven NAFLD. Apparently, healthy participants (n=100) were enrolled as a control group. Serum samples were tested for micro-RNAs− 122 and pro-neurotensin.Results: Compared with the control subjects, both mi-RNA-122 and serum proneurotensin levels were increased in NAFLD (p< 0.001) and at a cut-off ≥ 6.83, mi-RNA-122 had 51.0% sensitivity, 70.0% specificity to differentiate NAFLD from healthy controls, while serum proneurotensin had 80.0% sensitivity and 80.0% specificity at a cutoff ≥ 108.Conclusion: The circulating pro-neurotensin might be used as a novel biomarker for diagnosis of patients with NAFLD, wherefore the integration of a circulating mi-RNA-122 and serum pro-neurotensin could be beneficial to diagnose NAFLD cases. Large-scale studies are needed to investigate the possible role of mi-RNA-122 and pro-neurotensin in the development, progression, and prognosis of NAFLD and NASH.Keywords: nonalcoholic fatty liver disease, NAFLD, metabolic associated fatty liver disease, MAFLD, biomarker, proneurotensin, micro-RNA-122, noninvasive, diagnosis, fatty liver |
| first_indexed | 2024-03-12T05:19:20Z |
| format | Article |
| id | doaj.art-05718c2af7434fd0a9ca22b2864894b2 |
| institution | Directory Open Access Journal |
| issn | 1178-7007 |
| language | English |
| last_indexed | 2024-03-12T05:19:20Z |
| publishDate | 2022-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Diabetes, Metabolic Syndrome and Obesity |
| spelling | doaj.art-05718c2af7434fd0a9ca22b2864894b22023-09-03T07:46:30ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072022-06-01Volume 151935194376104Pro-Neurotensin as a Potential Novel Diagnostic Biomarker for Detection of Nonalcoholic Fatty Liver DiseaseMohamed AAAbo-Elmatty DMEzzat OMesbah NMAli NSAbd El Fatah ASAlsayed EHamada MHassnine AAAbd-Elsalam SAbdelghani AHassan MBFattah SAAmal A Mohamed,1 Dina M Abo-Elmatty,2 Omnia Ezzat,3 Noha M Mesbah,2 Nada S Ali,3 Aliaa Sayed Abd El Fatah,4 Eman Alsayed,5 Mahmoud Hamada,6 Alshymaa A Hassnine,7 Sherief Abd-Elsalam,8 Ahmed Abdelghani,9 Mohamed Badr Hassan,9 Shaimaa A Fattah2 1Biochemistry and Molecular Biology Department, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt; 2Biochemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt; 3Biochemistry Department, Faculty of Pharmacy, Egyptian Russian University, Cairo, Egypt; 4Internal Medicine Department, Faculty of Medicine, Minia University, Minia, Egypt; 5Department of Clinical Pathology, Minia University Hospital, Minia, Egypt; 6Internal Medicine Department, Faculty of Medicine, Benha University, Benha, Egypt; 7Department of Gastroenterology and Tropical Medicine, Faculty of Medicine, Minia University, Minia, Egypt; 8Tropical Medicine Department, Tanta University, Tanta, Egypt; 9Internal Medicine Department, Faculty of Medicine, Cairo University, Cairo, EgyptCorrespondence: Sherief Abd-Elsalam, Department of Tropical Medicine, Faculty of Medicine, Tanta University, El-Bahr Street, Tanta, Egypt, Tel +201147773440, Email sherif.abdelbaky@med.tanta.edu.egBackground and Aims: Currently, liver biopsy is the gold standard method for diagnosis of non-alcoholic fatty liver severity. It is critical to develop non-invasive diagnostic method to diagnose nonalcoholic fatty liver rather than invasive techniques. Our case–control study was to address the value of circulating miRNA-122 and serum pro-neurotensin as a potential non-invasive biomarker for the diagnosis of non-alcoholic fatty acid diseases.Methods: Clinical assessment, laboratory investigations, and anthropometric measurements were reported for 157 patients with proven NAFLD. Apparently, healthy participants (n=100) were enrolled as a control group. Serum samples were tested for micro-RNAs− 122 and pro-neurotensin.Results: Compared with the control subjects, both mi-RNA-122 and serum proneurotensin levels were increased in NAFLD (p< 0.001) and at a cut-off ≥ 6.83, mi-RNA-122 had 51.0% sensitivity, 70.0% specificity to differentiate NAFLD from healthy controls, while serum proneurotensin had 80.0% sensitivity and 80.0% specificity at a cutoff ≥ 108.Conclusion: The circulating pro-neurotensin might be used as a novel biomarker for diagnosis of patients with NAFLD, wherefore the integration of a circulating mi-RNA-122 and serum pro-neurotensin could be beneficial to diagnose NAFLD cases. Large-scale studies are needed to investigate the possible role of mi-RNA-122 and pro-neurotensin in the development, progression, and prognosis of NAFLD and NASH.Keywords: nonalcoholic fatty liver disease, NAFLD, metabolic associated fatty liver disease, MAFLD, biomarker, proneurotensin, micro-RNA-122, noninvasive, diagnosis, fatty liverhttps://www.dovepress.com/pro-neurotensin-as-a-potential-novel-diagnostic-biomarker-for-detectio-peer-reviewed-fulltext-article-DMSOnonalcoholic fatty liver disease (nafld)metabolic associated fatty liver disease (mafld)biomarkerproneurotensinmicro rna 122noninvasivediagnosisfatty liver. |
| spellingShingle | Mohamed AA Abo-Elmatty DM Ezzat O Mesbah NM Ali NS Abd El Fatah AS Alsayed E Hamada M Hassnine AA Abd-Elsalam S Abdelghani A Hassan MB Fattah SA Pro-Neurotensin as a Potential Novel Diagnostic Biomarker for Detection of Nonalcoholic Fatty Liver Disease Diabetes, Metabolic Syndrome and Obesity nonalcoholic fatty liver disease (nafld) metabolic associated fatty liver disease (mafld) biomarker proneurotensin micro rna 122 noninvasive diagnosis fatty liver. |
| title | Pro-Neurotensin as a Potential Novel Diagnostic Biomarker for Detection of Nonalcoholic Fatty Liver Disease |
| title_full | Pro-Neurotensin as a Potential Novel Diagnostic Biomarker for Detection of Nonalcoholic Fatty Liver Disease |
| title_fullStr | Pro-Neurotensin as a Potential Novel Diagnostic Biomarker for Detection of Nonalcoholic Fatty Liver Disease |
| title_full_unstemmed | Pro-Neurotensin as a Potential Novel Diagnostic Biomarker for Detection of Nonalcoholic Fatty Liver Disease |
| title_short | Pro-Neurotensin as a Potential Novel Diagnostic Biomarker for Detection of Nonalcoholic Fatty Liver Disease |
| title_sort | pro neurotensin as a potential novel diagnostic biomarker for detection of nonalcoholic fatty liver disease |
| topic | nonalcoholic fatty liver disease (nafld) metabolic associated fatty liver disease (mafld) biomarker proneurotensin micro rna 122 noninvasive diagnosis fatty liver. |
| url | https://www.dovepress.com/pro-neurotensin-as-a-potential-novel-diagnostic-biomarker-for-detectio-peer-reviewed-fulltext-article-DMSO |
| work_keys_str_mv | AT mohamedaa proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease AT aboelmattydm proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease AT ezzato proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease AT mesbahnm proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease AT alins proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease AT abdelfatahas proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease AT alsayede proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease AT hamadam proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease AT hassnineaa proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease AT abdelsalams proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease AT abdelghania proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease AT hassanmb proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease AT fattahsa proneurotensinasapotentialnoveldiagnosticbiomarkerfordetectionofnonalcoholicfattyliverdisease |