Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms
The effects of nodulation on N metabolism in soybean plants supplied with various forms of N are not fully understood. Ureides are the principal forms of N transported from nodules, but nitrate and asparagine are the primary N compounds transported from roots supplied with NO<sub>3</sub>...
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MDPI AG
2023-02-01
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| Series: | Metabolites |
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| author | Takuji Ohyama Miyuki Isaka Akihiro Saito Kyoko Higuchi |
| author_facet | Takuji Ohyama Miyuki Isaka Akihiro Saito Kyoko Higuchi |
| author_sort | Takuji Ohyama |
| collection | DOAJ |
| description | The effects of nodulation on N metabolism in soybean plants supplied with various forms of N are not fully understood. Ureides are the principal forms of N transported from nodules, but nitrate and asparagine are the primary N compounds transported from roots supplied with NO<sub>3</sub><sup>−</sup>. In this research, the effects of 1-day treatments of NO<sub>3</sub><sup>−</sup>, NH<sub>4</sub><sup>+</sup>, urea, or NO<sub>3</sub><sup>−</sup> + NH<sub>4</sub><sup>+</sup> on N metabolite concentrations in xylem sap and each organ were compared between nodulated and non-nodulated soybeans. Capillary electrophoresis and colorimetry were used for the analysis. In the xylem sap of the nodulated plants with an N-free solution, ureides were the major N metabolites, followed by asparagine and glutamine. Ureides concentrations were much lower in the xylem sap of the non-nodulated soybeans. In the NO<sub>3</sub><sup>−</sup> treatment, the concentrations of ureides in the xylem sap of the nodulated plants decreased compared to the control plants. In the NH<sub>4</sub><sup>+</sup>, urea, and NO<sub>3</sub><sup>−</sup> + NH<sub>4</sub><sup>+</sup> treatments, the concentrations of asparagine and glutamine increased significantly compared with the control and NO<sub>3</sub><sup>−</sup> treatments. Similar changes with the N treatments were observed between the nodulated and non-nodulated soybeans, suggesting that nodulation does not have significant effects on the metabolism of absorbed N in roots. |
| first_indexed | 2024-03-11T06:10:57Z |
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| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T06:10:57Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-05741f3f63e8485cacdc218f247d38412023-11-17T12:35:50ZengMDPI AGMetabolites2218-19892023-02-0113331910.3390/metabo13030319Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N FormsTakuji Ohyama0Miyuki Isaka1Akihiro Saito2Kyoko Higuchi3Department of Agricultural Chemistry, Faculty of Applied Biosciences, Tokyo University of Agriculture, Tokyo 156-8502, JapanDepartment of Agricultural Chemistry, Faculty of Applied Biosciences, Tokyo University of Agriculture, Tokyo 156-8502, JapanDepartment of Agricultural Chemistry, Faculty of Applied Biosciences, Tokyo University of Agriculture, Tokyo 156-8502, JapanDepartment of Agricultural Chemistry, Faculty of Applied Biosciences, Tokyo University of Agriculture, Tokyo 156-8502, JapanThe effects of nodulation on N metabolism in soybean plants supplied with various forms of N are not fully understood. Ureides are the principal forms of N transported from nodules, but nitrate and asparagine are the primary N compounds transported from roots supplied with NO<sub>3</sub><sup>−</sup>. In this research, the effects of 1-day treatments of NO<sub>3</sub><sup>−</sup>, NH<sub>4</sub><sup>+</sup>, urea, or NO<sub>3</sub><sup>−</sup> + NH<sub>4</sub><sup>+</sup> on N metabolite concentrations in xylem sap and each organ were compared between nodulated and non-nodulated soybeans. Capillary electrophoresis and colorimetry were used for the analysis. In the xylem sap of the nodulated plants with an N-free solution, ureides were the major N metabolites, followed by asparagine and glutamine. Ureides concentrations were much lower in the xylem sap of the non-nodulated soybeans. In the NO<sub>3</sub><sup>−</sup> treatment, the concentrations of ureides in the xylem sap of the nodulated plants decreased compared to the control plants. In the NH<sub>4</sub><sup>+</sup>, urea, and NO<sub>3</sub><sup>−</sup> + NH<sub>4</sub><sup>+</sup> treatments, the concentrations of asparagine and glutamine increased significantly compared with the control and NO<sub>3</sub><sup>−</sup> treatments. Similar changes with the N treatments were observed between the nodulated and non-nodulated soybeans, suggesting that nodulation does not have significant effects on the metabolism of absorbed N in roots.https://www.mdpi.com/2218-1989/13/3/319noduleallantoateallantoinasparaginecapillary electrophoresisglutamine |
| spellingShingle | Takuji Ohyama Miyuki Isaka Akihiro Saito Kyoko Higuchi Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms Metabolites nodule allantoate allantoin asparagine capillary electrophoresis glutamine |
| title | Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms |
| title_full | Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms |
| title_fullStr | Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms |
| title_full_unstemmed | Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms |
| title_short | Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms |
| title_sort | effects of nodulation on metabolite concentrations in xylem sap and in the organs of soybean plants supplied with different n forms |
| topic | nodule allantoate allantoin asparagine capillary electrophoresis glutamine |
| url | https://www.mdpi.com/2218-1989/13/3/319 |
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